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Summary of Contents for BioLAB BSDBU-301
- Page 1 OPERATING MANUAL DOUBLE BEAM UV VISIBLE SPECTROPHOTOMETER BSDBU-301 www.biolabscientific.com...
- Page 2 INDEX 1.Safety 2.Package Contents 3.Unpacking 4.Installation 4.1. Environment Required 4.2. Install Spectrophotometer 5.Overview 6.Symbols 7.Main Specifications 8.Description of Appearance and Keys 8.1.Appearance 8.2.Keypad 8.3.Description of Keys 9.Functions 9.1.Photometry 9.2.Quantitation 9.3.Wavelength Scan 9.4.Kinetics 9.5.DNA/Protein Measurement 9.6.Multi-wavelength 10.Getting Started 11.Important Guidelines 12.General Operating 13.Measuring 13.1.Photometry 13.2.Quantitation...
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Page 3: Table Of Contents
13.6.Multi Wavelength Mode 13.7.System Utility 14.Troubleshooting 15.Repair and Maintenance 15.1.Daily Maintain 15.2.Spare Parts Replacement 16.Warranty 17.Equipment Disposal... -
Page 4: Package Contents
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit. We recommends against the use of BSDBU-301 Spectrophotometer. Do not open the device. Disconnect the device from the mains supply before carrying out maintenance work or changing the fuses. -
Page 5: Installation
3.Unpacking Open the package, according to carefully check the packaging packing list items, if found inside the packaging are missing or damaged items please contact us and authorized contractual partners. 4.Installation 4.1. Environment Required To ensure the best performance, the following conditions are required: The best working temperature range is 16—35℃... - Page 6 5.Overview BSDBU-301 Spectrophotometer is an electrical measure instrument which is widely used in the laboratories. Use Frequency: Intermittence Excessive Voltage(Current): No Pollution Class: Class 1 6.Symbols The following chart is an illustrated glossary of the symbols that are used in this manual.
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Page 7: Main Specifications
7.Main Specifications Optical System Double beam Wavelength Range 190—1100nm Wavelength Accuracy ±0.3nm Wavelength Repeatability 0.2nm Photometric Range -0.3—3A, 0—200%T Photometric Accuracy ±0.3%T Photometric Repeatability 0.2%T Spectral Bandwidth 1.8nm Stray Light 0.05%T@220nm&360nm Stability ±0.001A/h@500nm Work Mode Photometry, Quantitation, Kinetics, Wavelength Scan, DNA/Protein, Multi Wavelength Interface USB, Parallel(printer) Power Requirement AC 110/220V, 50/60Hz Dimensions 600x450x260... - Page 8 8.Description of Appearance and Keys 8.1.Appearance Front View Back view www.biolabscientific.com...
- Page 9 1 LCD Display Lid of Sample Room 2 Sample holder 3 LCD Display 4 Keypad 5 USB Port(Type A, USB Memory) 6 Cover of Fan 7 Power Switch 8 Power Socket 9 LCD Contrast Adjust 10 Printer port 11 USB port (Type B, Communication) 8.2.Keypad www.biolabscientific.com...
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Page 11: Description Of Keys
8.3.Description of Keys Function Keys: Functions on-screen prompts … Numeric Keys: Enter numbers and letters CLEAR Key: Delete the input value or stored data ESC Key: Return to previous Interface 100%T/0Abs Key: Blank OPEN Key: Open files stored in internal memory SAVE Key: Save files to internal memory START/STOP Key: Start/Stop testing GOTO ʎ... - Page 12 UP, DOWN Keys: Scroll menu/data and set Y scale 9.Functions 9.1.Photometry Display results as Abs, %T or Concentration. 9.2.Quantitation We provide 2 methods to establish a Standard Curve: 2 methods to set up the regression curve. Up to 10 standard samples to calibrate the regression curve. 3 methods for curve fit.
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Page 13: Getting Started
10.Getting Started The following chart describes the basic operation of the instrument. Turn On and Self-check Switch on the power. Then the instrument begins to self-check and 15 minutes’ warm up. Self-check includes the following steps: Turn on lamps → Check Sensor → Initialize AD → System position → Get Dark Current → Warm up. www.biolabscientific.com... - Page 14 Finished self-check, “System calibration” interface displays, press the keys to select to do calibration or not. www.biolabscientific.com...
- Page 15 After system do or skip calibration, instrument displays Main Interface. www.biolabscientific.com...
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Page 16: Important Guidelines
11.Important Guidelines Reagents and dilution buffers can cause cauterization and other damage to health. Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious damage to health. During sample preparation, measuring procedures and maintenance and cleaning work, observe all local laboratory safety precautions (e.g. - Page 17 Print the Test Results Store the Test Results Press to store the test results, input the file name by numeric keys and press to save. If the USB Disk was installed, the data will save in the USB Disk, or the data will save in the internal memory. Load the Stored File In the test interface, press to go into file selecting interface,...
- Page 18 Method 1 ： Input Factor F Press to set F. Input the value of F by numeric keypad, press to confirm. Then the F value would display on the screen. Method 2 ： Use Standard Sample Put the reference sample in the light path and calibrate 100%T/0Abs; Put the standard sample in the light path ，...
- Page 19 Establish Standard Curve: Method 1: Input Regression Equation 1) Set Fit Curve Method. Press to set Fit method，use to choose the method and press to confirm. 2) Set Wavelength. Press to set wavelength. Use to choose measure method, then press to confirm.
- Page 20 Load the Stored Curves In the “Calibration Table” interface, press to go into files select interface. Use to select the curve you need and press to load. Users can press to view the curve. Step 4. Return the sample measurement interfaceIn the “Calibration Table” interface, press return the Quantitative Test interface.
- Page 21 13.3.Wavelength Scan Step 1. Enter into Wavelength Scan Main menu, press numeric key or to choose “Wavelength Scan” and press enter. Step 2. Parameters Setup Press to set parameters, set “Scan From” , “scan to”, “scan step”and “scan speed”, press confirm.
- Page 22 Step 5. Search Peaks After scanned, press to go into peak search mode. Press to set peak height, input the peak height and press to confirm.Press to display the value of every wavelength point. Press to display the value of every peak. Step 6.
- Page 23 confirm Step 5. Measure Samples Put the sample to be measured in the main light path and put Reference in the reference light path, press to begin the test, press to cancel. Step 6. Calculate Response Rate After time scanned, if users want to calculate the response rate of some period, you can press to go into “Process”...
- Page 24 13.5.DNA/Protein Mode Step 1. Enter into DNA/Protein Mode Main menu, press to choose “DNA/Protein Mode” and press to confirm. Step 2. Setup Parameters Press to set coefficients, input all the values of f1 to f4 by numeric keypad according the indication and press to confirm.
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Page 25: Multi Wavelength Mode
13.6.Multi Wavelength Mode Step 1. Enter into Multi Wavelength Measurement Main Menu, press to choose “Multi Wavelength Measurement” and then press to go into multi wavelength measurement interface. Step 2. Setup Wavelength Press to go into wavelength setting interface，input all the wavelength value one by one by the numeric keypad. -
Page 26: System Utility
13.7.System Utility Main menu, press or use to select “System Utility” and press to confirm. 13.7.1 Wavelength Reset Press or use to choose “Wavelength Reset” then press to begin the calibration. During the course, opening the lid of the compartment is prohibited. 13.7.2.Printer Setup Press to select “Printer Setup”... -
Page 27: Lamp Service
will resume the initial condition. Select print port Press or use to choose “Select print port” and press to confirm. Use to choose “LPT” or “Comm.” and then press to confirm. Select Printer Press or use to choose “Select printer” and press to confirm. -
Page 28: Clock Setup
press . Then users can find the W lamp’s usage time and the system will ask you if are sure to reset the usage time. Reset the W lamp usage time Press or use to choose “Reset W lamp usage time” and press . -
Page 29: Beeper On/Off
Press or use to choose “Display Date” and press to confirm. Then the Date will display on the Right Top corner. 13.7.5.Refresh Dark Curren Press or use to choose “Refresh Dark Current” and press to confirm. Then the system begins to refresh Dark Current. Note: During the course, open the lid of the compartment is prohibited. -
Page 30: Troubleshooting
14.Troubleshooting Review the information in the table below to troubleshoot operating problems. Problem Cause Solution Power cord connection is not reliable Improve connectivity Power on, no response Fuse burning Replace fuse Warm up is not enough Warm up more time Glass cuvettes used in UV Range Use quartz cuvettes Sample is not Stable... -
Page 31: Repair And Maintenance
15.Repair and Maintenance 15.1.Daily Maintain Check the Compartment After measurement, the cuvettes with sample solutions should be taken out of the compartment in time. Or the volatilization of the solution would make the mirror go moldy. Users must pay more attention to the corrosive sample and liquid easy to volatilize. - Page 32 Pick out the spare fuse (3.15A/250V) and replace it to the working position. Step 5. Reset the fuse seat Replace the fuse seat in the power socket. Step 6. Switch on the power Plug the socket and switch on the power. Replace Lamps www.biolabscientific.com...
- Page 33 Hot! Wait 20 minutes before open the lamp chamber after power off to avoid scald! Step 1. Tools preparation Prepare a 6×150mm Cross Blade screwdriver and a pair of glove. Step 2. Power Off Switch off the power supply and unplug the socket. Step 3.
- Page 34 Step 5. Replace the D2 lamp Unscrew the 2 screws on the D2 Flange (No.1), unplug the connector in the Power Board (No. 2) and remove the D2 lamp. Draw on the cotton glove and replace a new lamp. Fix the 2 screws and plug the connector again.
- Page 35 Step 7. Adjust the position of the W lamp Switch on the power(the Switch Mirror should be placed to the position as indicates). Observe the entrance facular, adjust the W Lamp until the light is in the center of the entrance hole. www.biolabscientific.com...
- Page 36 Step 8. Finish Reset the cover of the light chamber and fix the screws. Reset the cover of the instrument and fix the screws. Recover the Pole in the compartment, then the course finished. Replace the Battery Danger! Be sure to switch off the power and unplug the socket before replacement! Step 1.
- Page 37 Step 2. Switch off the power supply Switch off the power supply and unplug the socket. Step 3. Open the Bottom cover plate Unscrew all the screws indicated then remove the bottom plate. Step 4. Replace the Battery Pick out the old battery and replace a new one. www.biolabscientific.com...
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Page 38: Warranty
Step 5. Finish Recover the bottom plate and fix all the screws, then the course finishes. 16.Warranty We warrant that this product will be free from defects in material and 30 workmanship for a period of one (1) year from date of purchase. If a defect is present, we will, at its option, repair, replace, or refund the purchase price of this product at no charge to you, provided it is returned during the warranty period. -
Page 39: Equipment Disposal
By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health. Thank you！ Biolab Scientific Ltd. 3660 Midland Avenue, Suite 300, Toronto, Ontario M1V 0B8, Canada Email: info@biolabscientific.com | Website: www.biolabscientific.com...
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