1. Introduction MultiEX 024L is an automated nucleic acid extraction and purification system . It uses Magnetic Bead Separation technology, and has the capability of extracting and purifying nucleic acids from a variety of biological samples, such as whole blood, viruses, tissues, plants, animals, fungi, bacteria, cultured cells, swabs, feces, blood cards, etc.
adsorption force for nucleic acids, binds to the nucleic acid released on lysis. The nucleic acids bound to the magnetic beads can be transferred to different wells using the magnetic rods. The magnetic rod sleeves facilitate quick and even mixing of the buffer and the nucleic acid bound beads.
This symbol is used to indicate that certain precautions must be taken when working with potentially infectious material. For your safety and that of others, follow the guidelines provided in the following pages concerning the use of the MultiEx 024L system. WARNING: Ignoring the following may lead to fire or electric shock.
Doing so may cause fire or electric shock. • In case there is any liquid residue inside the device, wipe it up a soft paper tissue, etc. • Repairs to the MultiEx 024L system should only be performed by personnel specifically authorized by Manufacturer.
HOT SURFACE • Touching the heating pads while in operation may cause burn injury. User shouldn’t touch the heating blocks during or immediately after operation. Regarding Reagent Kit: CAUTION! Note: The kits are generally not supplied with the Device. Select the appropriate kit to suit your requirements.
3. Unpacking & Installation 3.1 Unpacking Unpack the device carefully and check for any damages which may have arisen during transit. Please contact the manufacturer/supplier for technical support. Note: If there is any apparent damage to the device, please do not connect the power line 3.1.1 Contents of the Package Item...
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3.1.2 System Structure This automatic nucleic acid extraction system consists of main housing, mechanical movement mechanism, magnetic rods, color touch screen, UV sterilizer, heating and temperature control modules, and control system. ① USB port ② Glass panel ③ 10-inch touchscreen ④...
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3.2 Device Installation and Setting Up 3.2.1 Operating conditions This automatic nucleic acid extractor must be installed and used indoors in environment that meets the following conditions: • Environment temperature: 10°C ~ 40°C • Relative humidity: 30% ~ 80% • Power supply: 200-240VAC, 100-120VAC; 50/60Hz •...
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3.2.3. System Specifications Model MultiEX 024L Throughput 1-24 samples Processing volume 50ul-8ml Processing time 20-40min/run (depending on reagent and volumes) Capacity Supports up to 24 Reagent strips or 6 plates Reagent Strips (Single Sample) / Strip Plates Consumables (4 Samples) and Magnetic Rod Sleeves...
4. Operating Instructions 4.1 Power connection Connect one end of the power cord to the power port on the back of the instrument and the other end to the power socket, then turn on power. 4.2. Installation and Removal of Magnetic Rod Sleeves Installing the sleeves: before run, load the magnetic rod sleeves into the second well.
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4.3 Installing the deep-well plates Open the door, click the "Tray out" button, and place the deep well palets on to the heating tray. Click "Tray in" button and close the door.
5. User Interface 5.1 Start up Close the door, and turn on the power switch on the back of the instrument and wait for the start-up display. The Home Screen has Five Main Menus which can be accessed from the side bar: 1.
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5.2 Create & Edit Programs To create or edit program, press the ‘Edit Prog.’ Icon on the Home screen side bar menu. ‘Edit Prog.’ Menu Options The following options can be accessed from the bottom menu bar • Create: To create new programs •...
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5.2.1‘Edit Prog.’ Instructions Creating New Programs: Programs can be created in two ways. • Option 1 -‘Create’ option: Use this option when creating an entirely new program, and while operating the instrument for the first time. Once the program is created all program parameters needs to be entered for each step.
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Edit Parameter: After creating the new program, you need to set corresponding para -meters for each step by clicking “Edit” button. Scroll down to the bottom of the Program List to see the newly created program and click to select the program. Then click the “Edit” button on the bottom menu to edit program parameters.
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After copying the program, click the “Paste” button in the bottom menu to create a new program by replicating the program parameters of the old program. You will be prompted to rename the program on clicking the “Paste” button. Enter new name and click ‘Confirm‘.
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The newly renamed program will appear just below the copied program. Click on the program to select. Then click the “Edit” button on the bottom menu to edit program parameters. This will take you to the ‘Edit Parameters’ page. For detailed instructions on how to add steps and edit program parameters, please see section 5.2.1.3 Editing Program Parameters.
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• Add step: Create a new step • Delete step: Delete existing step • Copy Step: Copy an existing step to replicate • Paste Step: Paste a copied step • Auto check: Set Auto-Check Note: Long press any step to edit parameters Adding a Step Click “Add Step”...
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Copy step & Paste step (Copying and replicating an existing step) Useful if you want to copy an existing step and then edit step parameters. Choose a step, click “Copy step” and then “Paste step” to replicate the step. Once the new step is created edit step parameters by long pressing, to enter ‘Edit Step’.
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1800μL. • Mix Time: The time for mixing during this step • Mix Speed: Higher the speed level, the faster the stirring. • Magnet Lowering (Time): It is the time set for the magnetic rod head to move from the liquid level towards the bottom of the well at a constant speed. Generally, it should be more than 10 seconds, which is good for collecting magnetic beads.
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• Fan (Off/On): Set the value to "0" to keep the cooling fan off and set "1" to keep it on during the step. • Lysis: Sets the lysis temperature for each well of the deep-well plate • Elution: Sets the elution temperature for each well of the deep-well plate 5.3 Run Program All saved programs can be found under the 'Run Prog.' Menu on the Home Page.
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Run Status: The extraction status can be monitored from the'‘Run Status'page. When a program is running on a particular well, the corresponding well icon is highlighted in orange. The well icons in blue indicate that Program Parameters are set for this well, and a gray well icon(s) indicates that no program parameters are set for this well.
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Nucleic acid extraction progress bar. 5.2.2.2 Functions on the ‘'Run Status'Page :Starts the program. :Pauses the program at the current running position. Click again to resume. :Ends the program and all moving components go back to initial position. :Turns On/Off the light to illuminate the extraction area :Turns On/off UV sterilization :Click ‘Parameters’...
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5.4.1 Log Click the date on to view the corresponding working record. The detailed steps, running times and errors are recorded. Export: Click , and select initial date and end date and click “Confirm” to download the data to USB. (Export format is TXT)
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5.4.2 Auto Check Choose the auto checks you want to perform by selecting the corresponding options. Unselect the options you don’t want to check. 5.4.3 Manual mode The manual mode is intended for use by trained service professionals and is not recommended for use by the customer.
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5.4.4 Light The light in the extraction chamber can be turned on or off with this setting 5.4.5 Upgrade (Software Upgrade): Both Interface Software and Control Software can be updated here. Insert the USB drive after saving the installation package on to the drive (Name of Interface Software Upgrade: update.apk;...
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5.4.6 Settings (Ventilation & Sound Alert & Pre-Heat Settings) Ventilation Fan Control: Turn on/off the fan near the heating platform. Sound Alert: The user can mute or unmute the sound alerts (error alert & beep alert when extraction is finished) Pre-Heat Setting for Blocks: Control the block temperature after the system starts or after the extraction completes.
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5.4.8 Time & Lang (Time/Language settings) Set the time. Choose the language between Chinese and English 5.4.9 Calibration Mode The calibration is rarely required and is done only when there is some change in sensor position. Entering the calibration mode requires a password. There are 6 calibrations: “Gap calibration between magnetic rods and magnetic rod sleeves”, “Well Calibration”, “Calibration of the gap between the magnetic rod sleeves and the deep well plate bottoms”, “Temperature calibration”, “Magnetic rod sleeves loading calibration”...
UV sterilization is completed. 6. Consumable List Catalog No. Description MultiEX-LS1 Large Volume reagent strip for 1 sample (DNase/RNase free, Non-Sterile) MultiEX-LS4 Large Volume reagent plate for 4 samples (DNase/RNase free, Non-Sterile) MRS-024L Magnetic Rod Sleeve for MultiEx 024L (DNase/RNase free, Non-Sterile)
7. Trouble shooting Troubleshooting and solutions Causes Solution Issue Power cord isn’t plugged in correctly Plug in the power cord correctly No display on Power isn’t connected Check and connect power screen after Blown Fuse powered on Contact supplier Power indication light is off ‘System Hang’...
8. Maintenance and Care • The heating pads and magnetic rod sleeves should be cleaned regularly with 75% ethanol and be disinfected by UV light for more than 30 minutes. Avoid contact with strong corrosive liquids and mechanical impact. • When the device isn't in regular use, it is recommended to do a trial run every 30 days. •...
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