Nikon ECLIPSE E600 Instructions Manual page 13

2. Microscopic Procedure
Close the field diaphragm
so that it is just outside the
lose th denser
field of view. (p. 19)
EC
e conden
aperture diaphragm.
Close the condenser
aperture diaphragm to
¿about 70 — 80% of the
numerical aperture of the
objective. (p. 18)
I] Close the field diaphragm.
Cura portion of the field of view is dark, adjust the condenser centering screws. If that does not resolve
the problem, check the following items:
* Filter insertion/removal (p. 20)
¢ Optical path selection for the eyepiece tube (p. 15)
* Position of the revolving nosepiece (p. 11)
* Revolving nosepiece installation (p. 28)
+ Lamp installation (p. 26)
* Condenser focusing and centering (p. 17)
+ Field and aperture diaphragms (p. 18 and 19)
¢ Condenser installation (p. 27)
M If it is not possible to focus on the specimen, check the following items:
* Mounting of the specimen (p. 31 and 32)
* Release of the coarse focus stopper (p. 14)
¢ Thickness of the cover glass (0.17 mm is standard) (p. 31 and 32)
12