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Related Manuals for Leica FluoCombi III
Summary of Contents for Leica FluoCombi III
- Page 1 Leica ™ FluoCombi III User manual...
- Page 2 Should you ever have any questions, please consult your local Leica representative. We are gladly at your service. Customer service is a big thing with us. Not only before the sale, but afterwards as well.
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Page 3: Overviews
Overviews Fig. 1 Leica FluoCombi III , 5× planapo HR objective rotated into the beam path Operating and function elements 1 Fine focusing with mount thread for a 5× or 20× HR objective (requires an adapter) 2 Clamping screw for locking the fine focusing... - Page 4 6 Clamping screw for fastening the UV protection screen 7 Clamping screw for locking the UV protection screen in the desired tilt position 8 Clamping screw for fastening the optics carrier in the microscope carrier of the FluoCombi III™ 9 Clamping screw for fastening the focusing stop...
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Page 5: Table Of Contents
2.6 Transport anchorage ....11 2.7 FluoCombi III™ and focusing drive ..11 2.8 Optics carrier ......12 2.9 Objectives . -
Page 6: General Notes
To maintain the unit in its original condition and to fluorescence images. Every existing instrument ensure safe operation, the user must follow the can be fitted with the Leica FluoCombi III™ in a instructions and warnings contained in these user few simple steps. -
Page 7: Assembly
10 447 085 20x HR 10 447 157 Optem / Mitutoyo Plan/Planapo 10 447 243 5x HR 10 447 185 500mm 10 445 631 10 445 363 10 445 387 10 445 367 HL 10 446 352 HL RC Leica FluoCombi III™ – Assembly... -
Page 8: Standard Delivery
– 1× Plan or planapo objective (7.9) – Dichroic mirror of your choice (Fig. 6) Assembly and setup of Leica MZ16 F, MZ16 FA fluorescence stereomicroscope and the fluores- cence illumination are described in the respective Fig. 5 Adapter (1) for Mitutoyo/Optem objectives operating instructions (see p. -
Page 9: Assembly Sequence
Fig. 7 Leica MZ16 F with Leica FluoCombi III™ 2.3 Assembly sequence 1 Base of stand 9 Plan or planapo objective 1× 2 Focusing drive with column (manual or 10 HR planapo objective 5× (or adapter and motorized) and column adapter (2a) -
Page 10: Column With Focusing Drive
– if the lowest focus position is approached too quickly using the motor focus, the fingers of the operator could be pinched between objective and object. Fig. 9 Leica FluoCombi III™ from below 1 Transport safety screws Leica FluoCombi III™ – Assembly... -
Page 11: Transport Anchorage
Only in this position is the thread accessible for mounting the FluoCombi III™ on the focusing drive. Fit in and hold FluoCombi III™ (10.2) in the two plugs (at the back of the yoke, not visible in the figure) on the focusing drive. -
Page 12: Optics Carrier
Fig. 11 Leica FluoCombi III™ with Leica MZ16 F 1 Clamping screw for fastening 4 Plan or planapo objective 1× the optics carrier 5 Thread for HR objective 2 MZ16 F, MZ16 FA optics carrier 6 Fine focusing 3 Thread for plan or planapo objective 1×... -
Page 13: Objectives
Tighten the 5× HR objective counterclockwise rotated in the microscope carrier of the in the thread mount. FluoCombi III™ since the 3rd beam path of the stereomicroscope and the FluoCombi III™ are adjusted to each other. Tighten the adapter (13.1) for Mitutoyo/Optem Loosen the clamping screw (11.1) and remove... -
Page 14: Uv Protection Screen
1 UV protection screen with holder 2 Mount for UV protection screen 3 Clamping screw for fastening the UV protection screen 4 Clamping screw for locking the UV protection screen in the desired tilt 5 Slot with dichroic mirror Leica FluoCombi III™ – Assembly... -
Page 15: Operation
• Zooming, focusing and adjusting interpupillary distance, eyecups, diopter settings, working distance, illumination see M2-105-0 or M2-160-4 (MZ16 FA) and M2-160-5 (MZ16 F) operating instructions. The following settings must be performed by every user only once. Leica FluoCombi III™ – Operation... - Page 16 (parfocal). If this is not the case, repeat the procedure. Fig. 15 Planapo objective 1× rotated into the beam path 1 Planapo objective 1× 2 Working distance 3 HR planapo objective 5× 4 Focusing stop Leica FluoCombi III™ – Operation...
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Page 17: Magnification
(parcentric) in stereo and micro mode for every zoom position. Fig. 16 HR planapo objective 5× rotated into the beam path 1 HR planapo objective 5× 2 Fine focusing 3 Planapo objective 1× Leica FluoCombi III™ – Operation... -
Page 18: Appendix
GFP filter set 0.04kg UV filter set 0.06kg Lamp housing 106Z for 50 W 2.26kg Lamp housing 106Z for 100 W 2.4kg Hg lamp 50 W 0.1kg Hg lamp 100 W 0.08kg Stray-light protection 0.58kg Leica FluoCombi III™ – Operation... -
Page 19: Dimensions
4.2 Dimensions HR planapo objective 5 × Leica FluoCombi III™ – Appendix... - Page 20 Leica FluoCombi III Dimensions in mm Leica FluoCombi III™ – Appendix...
- Page 21 201.8 105.84 Ø5.5 32.5 Dimensions in mm Leica FluoCombi III™ – Appendix...
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Page 22: Optical Data
4.3 Optical data Leica MZ16 F and MZ16 FA Objective Planapo 5 /0.5 (10447243) × Factor × Iris diaphragm open Working distance 19.8mm Eyepiece /21B × Field of view Numerical Resolution Visible Zoom Total position magnification diameter aperture (lp/mm) structure width (mm) (µm) - Page 23 1.98 128× 0.20 1.63 160× 0.24 1.37 205× 1.13 256× 0.87 0.34 1032 0.97 320× 1200 0.83 403× 0.56 0.45 1344 0.74 512× 0.44 0.49 1488 0.67 640× 0.35 1500 0.67 11.5 736× 1500 0.67 Leica FluoCombi III™ – Appendix...
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Page 24: Technical Data
4.4 Technical data Leica FluoCombi III™ Type Accessories with quick changer for Leica fluorescence stereomicroscopes MZ16 FA, MZ16 F, for a stereomicroscope objective (3D-view) and an HR objective (microscopic resolution) Optics Multiple-coated high-performance optics, lead-free, maximum resolution with micro objective; beam splitter for binocular observation Mechanics 360°... - Page 25 Specialty stages – Gliding stage – Thermocontrol System Leica MATS Accessories – FireWire digital camera systems for fluorescence – Analog and video cameras – Image processing and analysis software (IM1000, FW4000, QWin) – Measurement graticules Leica FluoCombi III™ – Appendix...
- Page 26 Leica Microsystems – the brand for outstanding products Leica Microsystems’ mission is to be the world’s first-choice provider of innovative The companies of the Leica Microsystems Group operate internationally in four business solutions to our customers’ needs for vision, measurement, lithography and analysis segments, where we rank with the market of microstructures.