LW Scientific Revelation III LED - Microscope Manual

Unpacking and Setup

LW Scientific packs each Revelation III Microscope with utmost care. Examine the outer and inner containers for any visual damage. Retain all of the packing material until you have examined and tested your new microscope. If there is damage, please contact the shipping company, as our warranty does not cover shipping damage. If you are uncertain who the shipper is, contact the distributor from whom you purchased the microscope. Please retain all packaging material for future use. Carefully unpack** your Revelation III Microscope using the following checklist for all the parts and accessories:

1 - Microscope body with Abbe condenser
2 - 10x eyepieces
1 - Binocular head (Seidentopf style)
1 - Mirror and attachment device for field use
2 - Filters (blue & green)
4 - Objectives 4X, 10XR, 40XR, 100XR (oil)
1 - Power Adapter
1 - Warranty Card
1 - Immersion oil
1 - Dust cover
**Note: Some parts may be packed in the outer recesses of the Styrofoam blocks

Assembly

1. Remove the body of the microscope and place it on a sturdy, dust-free surface. Remove the plastic plugs in the nose piece. Install the objectives in such a way that when you turn the nosepiece clockwise, you are moving from the 4x,
2. Remove the microscope head from the Styrofoam carton and pull off the protective covers from the eyepiece tubes and head mount. Insert the head mount into the upper arm of the body. Using the head retention screw, secure the head in place. Note: Do not over-tighten.
3. Insert the 10x eyepieces.
4. For protection during shipping your Rev III is shipped with the stage locked in low position. To disengage the lock, pull the STAGE LOCK LEVER towards the rear and downward. The stage should now move upward freely.
   
5. Attach the power adapter.

Power

If you suspect faulty electronics, call LW Scientific's technical service department at 800-726-7345.

USE ONLY SUPPLIED POWER ADAPTER

Power Adapter:
Input: 90-240V / 50-60 Hz Auto-Switching
Output: 12V1A
Microscope:
Input: 12V1A

Operation

1. Once you have assembled all the parts and allowed your microscope to come to room temperature, plug the power adapter into the microscope and then into the appropriate AC outlet. Note: Excess cold can fog lenses and cause lamp to fail.
2. Turn the light on using the black on/off switch on the right side of the base. Next adjust the light intensity using the brightness control wheel located on the right side just behind the on/off switch.
3. In order to become acquainted with the controls, choose a specimen slide with which you are familiar. For example, an old hematology slide or a commercially prepared slide. Place the slide into the slide holder by pushing back on the thumb guard to open the slide finger. The slide finger closes slowly to eliminate the possibility of chipping the corner of your slide when it closes.
4. Move the slide to the center of the stage, by turning the stage control knobs, located just below the stage. These knobs allow yo to move the slide on the X-Y axis (forward/backward and left/right).
5. The sub-stage iris should then be set to match the aperture of the objective for maximum resolution under each objective power. You should begin with the 4x or 10x objective. 4x objective 1.0 N.A. (nearly closed) 40x objective - 0.65 N.A. (halfway closed) 10x objective - 0.25 N.A. (1/4 closed) 100x objective - 1.25 N.A. (wide open)
6. Insert the filter of your choice into the swing-out filter holder beneath the Abbe condenser. Note that many customers prefer to use the blue filter for routine use.
7. Once you are comfortably seated, look into the oculars and move the eyepiece tubes together or apart until you see only one complete circle of light. You have now adjusted your interpupillary distance.
8. Using the 4x or 10x objectives and the coarse and fine adjustment knobs, bring the specimen into focus. Now, move the 40x objective into place. You will feel a "clicking" action when the objective is seated properly. Again, adjust focus for best image. You should also adjust the iris diaphragm (as listed above) for the best contrast and resolution.
9. Diopter Adjustment: Since you are using a binocular microscope, you have to adjust the normal difference in vision between your two eyes. This is a simple but critical adjustment! Close your left eye and look into the right ocular with your right eye. Adjust the focus to give you the best image. Now look at the ocular tube on the left. You will see that the left ocular tube has a built-in adjustment ring. Now close your right eye and look with your left eye into the left ocular. Using the diopter adjustment ring on the left ocular tube, adjust the focus until you see a clear, focused field.
10. Friction Adjustment: With repeated use and wear, the stage may drift out of focus. If this happens, you need only to tighten the tension control ring (located on the right side of the microscope between the coarse adjustment and the body of the microscope). If the coarse focus is hard to turn, you may choose to loosen the friction adjustment.
11. Stage Stop Lever: To help prevent the stage from hitting the objectives, the Revelation III Microscope is equipped with an adjustable stage stop. Rotate the 100x oil objective into place, and put a slide into the slide holder. Slowly raise the stage, stopping when the slide makes contact with the objective. Now, turn the stage stop lever in a clockwise direction toward you. The stage stop lever is located on the left side of the microscope between the coarse adjustment and the body of the microscope.

Maintenance

1. Always cover your microscope with the dust cover when not in use.
2. When cleaning the lenses, use lens paper or a cotton swab dipped in lens cleaning solution.
3. Excess oil should be cleaned off your 100x objective and stage at once. An alcohol pad is best for removing oil from the stage and on the other metal parts, but is not recommended for use on the lenses. Use lens cleaning solution and lens paper to clean off your objectives.
4. Dust in the nosepiece or ocular tubes should be blown out using filtered air. Canned air dusters work well for this job.
5. Whenever you remove an objective, we recommend that you place it back into the original plastic shipping vial until ready to be placed back on the microscope. SCREW THE OBJECTIVE SECURELY INTO THE CAP OF THE HOLDER - DO NOT DROP OBJECTIVE LOOSELY INTO CONTAINER.
6. To keep your microscope in top condition for years, LW Scientific recommends that you have the microscope professionally serviced once a year. Warning: The 40x objective is not sealed for oil immersion. Damage to the 40x objective due to oil immersion is not covered under warranty.

Specifications

Nosepiece
Quadruple hole multiple ball bearing

Head
Binocular (Seidentopf)
Inclined 30°, rotates 360°
Single diopter adjustment
10X/18 wide-field eyepieces
Monocular or Trinocular available

Illumination
Moveable Abbe Condenser (NA 1.25)
Iris Diaphragm
Swing-out filter holder with blue and green filters LED light

Operating Environment
Indoor Use Only
Ambient temperature: 5˚ to 40˚C (41˚ to 104˚F)

Stage
Mechanical stage
(140 mm x 140 mm) – coaxial drive controls
Range of traverse is 73 mm x 43 mm
Acid and reagent resistant finish

Dimensions and Weight
Weight: 14 lbs / 6.4 kg
Height: 15" / 380 mm
Length: 9" / 230 mm
Width: 7" / 178 mm
Shipping weight: 17 lbs/ 8kg

Objectives
DIN Achromatic or DIN Plan
4X N.A. 0.10
10X N.A. 0.25
40XR N.A. 0.65
100XR N.A. 1.25 (oil immersion)
20X, 50X oil Plan, and 60XR available

Adjustment Controls
Eyepiece: Interpupillary distance adjustment 55-75 mm
Stage Controls: Knobs allow movement of slide on X-Y axis
Etched vernier scales
Coarse Adjustment: Range of 30 mm
Fine Adjustment: Graduation of 2µm
Variable Light Adjustment

Construction
Rugged alloy

Documents / Resources

Download manual

Here you can download full pdf version of manual, it may contain additional safety instructions, warranty information, FCC rules, etc.

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