High Separation Physiological Fluid Analysis Method - Hitachi L-8900 Service Manual

1.5.2 High Separation Physiological Fluid Analysis Method

No. Item
1 Part number
2 Column size
3 Packing material
4 Separation degree JP
5 Analysis pressure
6 Analysis temperature
7 Column replacement (rough standard)
8 Reproducibility (retention
(The same column, the same lot of
reagent are used.)
9 Reproducibility (Peak area)
The same column, the same lot of
reagent are used.
10 Analysis time
No. Item
1 Part number
2 Buffer solution constitution
1. Specifications for analysis column and analysis method
2. Specifications for reagent
Contents
855-3408 L-8500 PF kit
Contents
PF-1: 2 pieces
PF-2: 1 piece
PF-3: 1 piece
PF-4: 2 pieces
PF-RG: 1 piece
B1: PF-1: 700 mL, EtOH: 9 mL, Distilled water:
291 mL
(Preparation way is explained in the instruction
manual.)
B2: The same specifications as the former PF-2.
B3: The same specifications as the former PF-3.
B4: The same specifications as the former PF-4.
B5: The same specifications as the former PF-
RG.
1 - 12
Contents
855-4350
6.0 mm ID × (25 × 25) mm
Cation exchange resin #2622 (Li type)
Thr/Ser: 1.2 or more
Gly/Ala: 1.2 or more
Ile/Leu: 1.0 more
10 MPa or less
30 - 70 °C
500 samples of water solution
Ala: Within CV 0.5%
Arg: Within CV 0.3%
Gly: Within CV 1.5%
His: Within CV 1.5%
Net: 120 min or less
Cycle: 150 min ± 3 min