Sealing Parameters - 4titude 4s2 Manual

9

SEALING PARAMETERS

The following sealing temperatures are for guidance only. Sealing
efficiency varies depending on the plate type used.
Add 1 second to the sealing time when a weighted platen is
Part Description
number
4ti-0541 Clear Seal
4ti-0575 Clear Weld Seal Mk II
4ti-0581 Clear Seal 3730
4ti-0521 Peel Seal
4ti-0587 DMSO Resistant Peel Seal
4ti-0531 Pierce Seal
4ti-0536 Foil Seal
4ti-0547 PS Foil Seal
4ti-0527 Black Seal
4ti-0591 Thermal Bond
4ti-0595 Gas Permeable Heat Seal
Table 2 - Sealing Temperatures and Times for different types of plates
* These values are for two-component PCR plates. For one-piece polypropylene
plates, increase dwell time by one second.
^
These values can be used for all polystyrene plates
All sealing parameters were estimated using software version 3.03
These values are for guidance only. It is advisable to conduct trials
depending on the plate type being sealed.
4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
Tel: +44 (0) 1306 884885 Fax: +44 (0) 1306 884886 info@4ti.co.uk www.4ti.co.uk
-0602)
used (4ti
Temperature (° C) / Dwell Time (secs)
96-well
PCR Plates*
175-185 / 2-3
175-180 / 2-3 170-175 / 2-3
165-175 / 3
175-185 / 3
175-185 / 3
160-175 / 2
165-180 / 2
-
-
170-180 / 2-3
165-175 / 3
384-well Vision
PCR Plates* Plates™
165-180 / 3 185-200 / 3
165-175 / 2 175-185 / 2-3
170-175 / 2-3
170-175 / 2-3
160-175 / 2 185-200 / 3
160-175 / 2-3 185-200 / 3
- 185-200 / 3
- 185-200 / 3
160-170 / 2
165-175 / 3 175-185 / 3
^
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