Shodex OHpak SB-2000 Series Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex OHpak SB-2000 series is size exclusion
chromatography columns used with aqueous solvents. The column series is suitable for preparative isolation of
various water-soluble polymers, proteins, and oligomers. Please select columns with target molecular weight
ranges (see below) that meet the molecular weight range of your samples.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
OHpak SB-2002
F6516011
F6516012 OHpak SB-2002.5
F6516013 OHpak SB-2003
F6516014 OHpak SB-2004
F6516015 OHpak SB-2005
OHpak SB-2006
F6516016
OHpak SB-2006M
F6516017
OHpak SB-G 8B
F6709555
SB-2006M is a mixed-gel column, provides a wider molecular-weight analysis range.
Operation Manual
Shodex OHpak SB-2000 series
Column Size (mm)
I.D Length
20.0 300
20.0 300
20.0 300
20.0 300
20.0 300
20.0 300
20.0 300
8.0 50
Resonac Corporation (https://www.shodex.com/)
Theoretical
Particle Size
Plate Number
(μm)
(Per Column)
15 ≥ 9,000
10 ≥ 12,000
10 ≥ 12,000
18 ≥ 12,000
20 ≥ 12,000
20 ≥ 12,000
20 ≥ 12,000
18 (Guard Column)
Reference value only / Measured with pullulan
*1
ver.20221202E
Exclusion Limit *1
1,000
10,000
100,000
1,000,000
(4,000,000)
*2
(20,000,000)
*2
(20,000,000)
*2
-
Estimated value
*2
1