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STEINDORFF NYMCS-702 Instruction Manual

Inverted fluorescence microscope
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STEINDORFF
Inverted Fluorescence Microscope
NYMCS-702
Instruction Manual
This manual is written for Inverted Fluorescence Microscope NYMCS-702. For safety and for keeping the best
performance, making you familiar with the instrument entirely, it is strongly recommended that you read this
manual carefully before using the microscope.

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Summary of Contents for STEINDORFF NYMCS-702

  • Page 1 NYMCS-702 Instruction Manual This manual is written for Inverted Fluorescence Microscope NYMCS-702. For safety and for keeping the best performance, making you familiar with the instrument entirely, it is strongly recommended that you read this manual carefully before using the microscope.

  • Page 2: Table Of Contents

    ® STEINDORFF CONTENTS User Notice ……………………………………………………………………………………………….. Component Names …………………………………………………………………………………... Installation …………………………………………………………………………………………… 2.1. Installing diagram ……………………………………………………………………………... 2.2. Installment steps ……………………………………………………………………………….. Adjustment and operation …………………………………………………………………………... 3.1. Lamp adjustment for fluorescence observation ……………………………………………... 3.2. Reflected observing illumination adjustment ………………………………………………... Microscope photography and video ………………………………………………………………...

  • Page 3: User Notice

    ® STEINDORFF USER NOTICE Safety Note 1. The epi-fluorescent attachment is a precise instrument. Open the box carefully, and avoid dropping the accessories to ground and causing damage to them. 2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and variations.
  • Page 4 ® STEINDORFF Maintenance and Storage 1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep these chemicals away from open flames or potential sources of electrical sparks-for example, electrical equipment that is being switched on or off.

  • Page 5: Component Names

    ® STEINDORFF 1. COMPONENT NAMES Phase contrast slider Lamp Trinocular tube head Eyepiece Illumination bracket Condenser Protection barrier Stage Stage inserted plate Illuminator Objectives o 4× Nosepiece o 10× o 20× o 40× Body Reflected fluorescence units Fluorescence power supplier...

  • Page 6: Installation

    ® STEINDORFF 2. INSTALLATION 2.1. Installing diagram The following figure shows the installation sequence of the components. The number in the figure shows the installation steps.  Before installing, be sure every components is clean, do not score any parts or glass surface.

  • Page 7: Installment Steps

    ® STEINDORFF 2.2. Installing Steps 2.2.1. Installing trinocular head (Figure 1) Loosen the setscrew ② and insert the trinocular Viewing Head ① into the body correctly, screw down with bolt ③. Figure 1 2.2.2. Installing eyepiece (Figure 2) Insert the eyepiece ④ into tube until they are against.
  • Page 8 ® STEINDORFF 2.2.4. Installing lamp house (Figure 4, Figure 5) Insert the plug “A” into hole “A” of power cord, then insert the plug B into the B hole of the condenser till there are against. (Figure 6) Replace lamp Turn the switch to off position when using or need replacement.
  • Page 9 ® STEINDORFF 2.2.6. Installing the objective (Figure 8, Figure 9) 1. Turning the coarse fusing knob ① like the figure shows till the nosepiece get to its lowest position.  For ensuring the safety of the instruction on transportation, the nosepiece is located in the lowest position and the tension adjustment collar ②...
  • Page 10 ® STEINDORFF  Be sure to use a GCQ-100 mercury burner.  Be sure to mount positive pole (the wide head) before the other, or the damage to the burner may occur.  Never subject the burner to excessive force when mounting the Mercury Burner.
  • Page 11 ® STEINDORFF 2.2.10. Installing the stage lengthen splint and the mechanical ruler (Figure 14, 15) Stage lengthen splint can be installed in either side of the stage to enlarge the work surface. But you can’t install the mechanical ruler together.

  • Page 12: Adjustment And Operation

    ® STEINDORFF 3. ADJUSTMENT AND OPERATION 3.1. Lamp adjustment for fluorescence observation 3.1.1. Connecting power Set the main switch of the power supply unit to “I” (ON). It will stabilize in 5 to 10 minutes after ignition.  Some mercury burners may not ignite the first time the power is turned on due to variance in production, and the safety mechanism in the starter in such a case.
  • Page 13 ® STEINDORFF 3.1.4. Adjusting the interpupillar distance (Figure 3, 4) When observing with two eyes, hold on the left and right prism holder, turn around the axis, adjusting the interpupillar distance until the left and right fields of view coincide completely.
  • Page 14 ® STEINDORFF 3.1.7. Centering the mercury burner (Figure 6-8) Before proceeding to center the burner, wait for the arc image to stabilize to protect against glare during arc image centering, it should be viewed across the excitation light protective shield.
  • Page 15 ® STEINDORFF Note:  When the hour counter indicates “100.0”, set the main switch to “o” (OFF) for safety, wait for more than 10 minutes, and then replace the lamp burner after making sure that the lamp housing has cooled down. A mercury burner seals high-pressure gas inside.
  • Page 16 ® STEINDORFF 3.1.8. Centering the Field Iris Diaphragm (Figure 9, 10) Engage the 10×objective in the light path, and place the specimen on the stage and bring into approximate focus. Pull the field iris diaphragm lever ② out until the diaphragm comes into the smallest state.
  • Page 17 ® STEINDORFF 3.1.9. Using color filters (Figure 11) Selecting the appropriate color filters according your need, it became more effective to observe or photography the specimen. Especially, we suggest using the LBD color filter, which can compensate more neutral colors.

  • Page 18: Reflected Observing Illumination Adjustment

    ® STEINDORFF 3.2. Reflected observing illumination adjustment 3.2.1. Turn power, adjusting brightness (Figure 13) Connect the power, turn on the main switch ① on the bottom side of the base to “－” (on). Turning the brightness adjustment knob clockwise ②, the voltage raise, and the brightness strengthen;...
  • Page 19 ® STEINDORFF  If the light ring is centering incorrectly, you will fail to obtain the best viewing effect of the microscopy.  After removing or replacing a thick specimen, the light ring and the phase contrast ring are likely to deviate each other, which will result in a decline of the image contrast.

  • Page 20: Microscope Photography And Video

    ® STEINDORFF 4. MICROSCOPE PHOTOGRAPHY AND VIDEO 4.1. Microscope video 4.1.1. Selecting the light path (Figure 1)  Just used in the trinocular observation Pulling out the light path selector lever, until you heard the “clicked”.  In dark specimen observation, you can make the focus by both eyes at first, then change the light path.

  • Page 21: Microscope Photography

    ® STEINDORFF 4.2. Microscope photography 4.2.1. Selecting the light path  Just used in the trinocular observation The operation diagram is shown in the details reference is in 4.1.1. 4.2.2. Installing the photography set (Figure 3) 1. Loosen the locking bolt ① on the trinocular viewing tube, and take out the dust cap ②.

  • Page 22: Outfit

    ® STEINDORFF 5. OUTFIT 5.1. Specifications Optical system Infinite optical system NYMCS-702 Excitation units excitation Dichroic mirror Barrier filter ● Blue Excitation DM500 BA520 BP460～490 Reflected light ● Green Excitation DM570 BA590 BP510～550 source ○ Ultraviolet Excitation DM400 BA420 BP330～385 ○...

  • Page 23: Objective Specifications

    ® STEINDORFF 5.2. Objective Specifications Numerical Working Conjugate Focus Cover Slip Type Magnification Aperture Distance Distance Distance Thickness (N.A.) (mm) (mm) (mm) ∞ ─ Infinite Long Working 25.2 Distance Plan ∞ Achromatic Objective 1.2mm ∞ Infinite Long Working 0.25 0.17 Distance Plan Phase ∞...

  • Page 24: Troubleshooting

    ® STEINDORFF 6. TROUBLESHOOTING Under certain condition, some no-fault factors will bring a reversible influence to the instrument’s performance. If the problem is happened, please take proper measures according to the follow table. If you can’t solve the trouble by the supplied methods, please contact with the sales department of our company.
  • Page 25 ® STEINDORFF The nosepiece is not in the center of the light Insure the nosepiece is in the “clicked” position path Place the specimen on the stage The specimen don’t place properly 6. One side of the image correctly. is unfocused...

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