Centering The Field Diaphragm; Effects Of The Field Diaphragm; Available Fluorescence Filter Cubes - Optika Italy IM Series Instruction Manual

9.2 Centering the field diaphragm
1. Place the specimen on the stage, insert 10x objective into the
light path and focus.
2. Rotate the field diaphragm lever
diaphragm. (Fig. 24)
3. Rotate the two centering screws
the center of the field of view.
4. Gradually open the diaphragm. The field diaphragm is
centered when the diaphragm image is symmetrical to the
field of view.
5. In normal use, open the diaphragm until it circumscribes the
field of view.
9.3 Effects of the field diaphragm
Field diaphragm adjusts the illuminated area to obtain a high
contrast image.
Set the diaphragm according to the objective in use until it
circumscribes the field of view, in order to eliminate unnecessary
light to eyepieces. (Fig. 25)
9.4 Available fluorescence filter cubes
• M-797
F I LT E R EXCITATION
NAME FILTER
B 460-490 nm
G 540-580 nm
• M-798
F I LT E R EXCITATION
NAME FILTER
B 460-490 nm
G 527-553 nm
UV 325-375 nm
V 390-420 nm
, to fully close the
①
to bring the bright spot in
②
D I C H R O I C BARRIER
MIRROR FILTER
495 nm 520LP nm
585 nm 590LP nm
D I C H R O I C BARRIER
MIRROR FILTER
500 nm 520LP nm
565 nm 575LP nm
415 nm 435LP nm
440 nm 455LP nm
• FITC: fluorescent antibodies
• Acridine orange: DNA, RNA
• Auramine
• Rhodamine, TRITC: fluorescent antibodies
• Propidium iodide: DNA, RNA
• RFP
• FITC: fluorescent antibodies
• Acridine orange: DNA, RNA
• Auramine
• Rhodamine, TRITC: fluorescent antibodies
• Propidium iodide: DNA, RNA
• RFP
• Nuclear counterstaining
• Acridine orange: DNA, RNA
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APPLICATIONS
APPLICATIONS
F ig. 24 F ig. 24
F ig. 25 F ig. 25