LaMotte SMART3 Soil Operator's Manual page 103

Colorimeter
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PROCEDURE
1. Press and hold
until colorimeter turns on.
ENTER
2. Press
to select Testing Menu.
3. Select All Tests (or another sequence containing 081 Phosphate LR) from Testing
Menu.
4. Scroll to and select 081 Phosphate LR from menu.
5. Use the 1 mL pipet (0354) to add 1 mL of soil extract to a clean tube (0290) and
dilute to the 10 mL line with deionized water. Cap tube and mix.
6. Insert tube into chamber, close lid and select Scan Blank.
7.
Remove tube from colorimeter. Use 1.0 mL pipet (0354) to add 1.0 mL of
*Phosphate Acid Reagent (V-6282). Cap and mix.
8. Use the 0.1 g spoon (0699) to add one measure of Phosphate Reducing Reagent
(V-6283). Cap and mx until powder dissolves. Wait 5 minutes for full color
development. Solution will turn blue if phosphates are present.
9. At end of 5 minute waiting period, mix, insert tube into chamber, close lid and select
Scan Sample. Multiply the result by 32 to determine the phosphorus concentration
in lb/acre.
10. Press
to turn colorimeter off or press
make another menu selection.
PHOSPHORUS IN ALKALINE SOILS
A special extraction procedure is used for determining the available phosphorus content
of Western U.S. alkaline soils where the pH value is above 7.0.
EXTRACTION PROCEDURE
1. Use the 1 mL pipet (0354) to add 1 mL of the *Special NF Extracting Solution
(6362) to the test tube (0701) then add deionized water to the 15mL
graduation.
2. Add 3 one gram measures of soil using the 1 g spoon (0697) to the extracting
solution in the vial.
3. Cap the vial and shake for a period of 5 minutes.
4. Filter using the funnel (0459) and fi lter paper (0463). Collect all of the fi ltrate.
5. Perform the Phosphorus test according to the Phosphorus procedure given
above.
Smart3 Soil Test Procedures 9.18
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EXIT
to exit to a previous menu or
PHOSPHATE
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