LaMotte smart spectro 2 Operator's Manual

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OPERATOR'S

MANUAL

2000-02-MN

05.02.16

GlobalScientificSupply

GLOBAL SCIENTIFIC SUPPLY

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Page 1 OPERATOR’S MANUAL 2000-02-MN 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 2  Standard Additions ........................ 15  Sample Dilution Techniques & Volumetric Measurements ......... 16  Interferences ........................... 17  Stray Light Interference ....................... 17 OPERATION OF THE SMART Spectro 2 SPECTROPHOTOMETER  Overview ............................ 18  Power Source ........................... 18  Components ..........................19 ...
Page 3  Send All Data ..........................76  Updata Programmed Tests ....................76 BATTERY OPERATION  Charging the Battery Pack ....................76  Running the SMART Spectro 2 Using the Battery ............77 MAINTENANCE  Cleaning ............................ 77  Lamp Replacement and Realignment ................77 ...
Page 4: Packaging & Delivery
Failure to do so could result in personal injury or damage to the equipment. The SMART Spectro 2 should not be stored or used in a wet or corrosive environment. Care should be taken to prevent water or reagent chemicals from wet spectrophotometer tubes from entering the SMART Spectro 2 chamber.
Page 5: Limits Of Liability
 LIMITS OF LIABILITY Under no circumstances shall LaMotte Company be liable for loss of life, property, proﬁ ts, or other damages incurred through the use or misuse of their products.  WARRANTY LaMotte Company warrants this instrument to be free of defects in parts and workmanship for 2 years from the date of shipment.
Page 6 The precision can be very good and the accuracy very bad. This is a useful measure of the performance of a test method. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 7 5. Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta, American Laboratory, Sept 2003, P. 34. Sensitivity: Sensitivity is the resolution based on how this term is used in LaMotte catalogs. This term is not listed in any of the references. Sometimes it is used for detection limit.
Page 8: Contents And Accessories
LaMotte  EPA COMPLIANCE The SMART Spectro 2 is an EPA-Accepted instrument. EPA-Accepted means that the instrument meets the requirements for instrumentation as found in test procedures that are approved for the National Primary Drinking Water Regulations (NPDWR) or National Pollutant Discharge Elimination System (NPDES) compliance monitoring programs.
Page 9: Chemical Testing Water Sampling For Chemical Analysis
For dissolved oxygen studies, or for tests requiring small sample sizes, a Water Sampler (LaMotte Code 1060) will serve as a sub-surface or in-depth sampler. This weighted device is lowered to the sampling depth and allowed to rest at this depth for a few minutes.
Page 10: Filtration
ﬁ ltration is an option. Reagent systems, whether EPA, Standard Methods, LaMotte or any others, will generally only determine dissolved constituents. Both EPA and Standard Methods suggest ﬁ ltration through a 0.45 micron ﬁ lter membrane, to remove turbidity, for the determination of dissolved constituents.** To test for total constituents,...
Page 11: An Introduction To Colorimetric Analysis & Spectroscopy
Most test substances in water are colorless and undetectable to the human eye. To test for their presence we must ﬁ nd a way to “see” them. The LaMotte SMART Spectro 2 can be used to measure any test substance that is itself colored or can be reacted to produce a color.
Page 12: Reagent Blank
Handle the tubes carefully, making sure the bottom half of the tube is not handled. LaMotte Company makes every eﬀ ort to provide high quality spectrophotometer tubes. However, wall thicknesses and diameter of tubes may still vary slightly. This may lead to slight variations in results (e.g.
Page 13: Calibration Curves
The SMART Spectro 2 contains precalibrated tests for the LaMotte reagent systems. The ﬁ rst step in using a non-LaMotte reagent system with the SMART Spectro 2 is to create a calibration curve for the reagent system. To create a calibration curve, prepare standard solutions of the test factor and use the reagent system to test the standard solutions with the SMART Spectro 2.
Page 14 Many computer spreadsheet programs can produce the curve of best ﬁ t by regression analysis of the standard solution data. A sample of each type of graph appears below: SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 15: Preparing Dilute Standard Solutions
The procedure can be repeated with larger and larger “spikes.” If the determined concentrations do not equal the concentration of the sample plus that added with the “spike”, then an interference may exist. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 16: Sample Dilution Techniques & Volumetric Measurements
 SAMPLE DILUTION TECHNIQUES & VOLUMETRIC MEASUREMENTS If a test result using the SMART Spectro 2 gives an OUT OF RANGE message then the sample concentration could be over range or under range. If it is over range, the sample must be diluted.
Page 17: Interferences
(see page 16).  STRAY LIGHT INTERFERENCE Normal indoor lighting causes no interference with the SMART Spectro 2. Always be sure the sample chamber lid is closed when scanning blanks or samples. SMART Spectro 2 Operator’s Manual 04.16...
Page 18: Power Source
To use the SMART Spectro 2 with an AC power supply: 1. Plug the Power Supply into the AC Adapter socket on the back of the SMART Spectro 2. 2. Connect the Power Cable to the Power Supply and an electrical outlet.
Page 19: Components
 COMPONENTS Figure 1 shows a diagram of the SMART Spectro 2 and the components. Lamp Replacement Access: For lamp replacement and realignment Power Input Socket: USB Port For 12V DC Power input 10mm Square Cell in Adapter 25mm Round Cell...
Page 20: Quick Start
 QUICK START 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds. After PROGRAMMED TESTS initialization the MAIN MENU will appear. %T/ABS PC LINK SYSTEM SETUP MAIN MENU 12:00:00 2. Press to scroll to PROGRAMMED TESTS.
Page 21 1 ALKALINITY-UDV 2 ALUMINUM 3 AMMONIA-N L F 4 AMMONIA-N L S 5 AMMONIA-N H 6 ARSENIC 7 BIGUANIDE 8 BORON SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 22 To print the test results, scroll to Test Result List. Press *ENTER to print. After obtaining test results, scroll with , and make another selection with *ENTER. Press EXIT to escape to previous menu. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 23: Sample Holders
This button turns the spectrophotometer oﬀ .  SAMPLE HOLDERS The SMART Spectro 2 is supplied with two removable sample cell holders. Each holder is secured in the chamber with a single screw. The square sample holder should be positioned so the arrow on the top is pointing toward the left. The square sample holder will hold 10 mm square cuvettes.
Page 24: The Display & The Menus
This list or menu is viewed through a window, the menu choice window, in the display. Pushing the arrow buttons brings another portion of the menu into menu choice window. This is referred to as scrolling through the menu. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 25: System Setup
It is used to refresh the system background. If the working conditions change, or the instrument has been in use for an extended length of time, refreshing the dark current will improve the stability. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 26: Calibrate Wl
The Calibrate Wavelength (CALIBRATE WL) mode is used to establish or re-establish the accuracy of the wavelength selection process. Normally, the Calibrate Wavelength procedure should be run after the SMART Spectro 2 is turned ON and allowed to warm up for 15 minutes or if operating conditions (temperature, humidity, etc.) change signiﬁ...
Page 27: System Setup Dark Current
BT PRINTER EDIT CLOCK STORE METHOD BEEPER ON/OFF CORRECTION WL LANGUAGE 5. Press *ENTER to select CALIBRATE WL. CALIBRATE WL CALIBRATE WL??? PLEASE SELECT SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 28: Bt Printer
System Setup Menu.  BT PRINTER Bluetooth wireless technology allows communication between the spectrophotometer and the Mobile Bluetooth Printer (Code 5-0066 only). The Bluetooth feature is always on. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 29 MAIN MENU 12:00:00 5-0066). PROGRAMMED TESTS %T/ABS PC LINK SYSTEM SETUP 2. Press ON to turn on the SMART Spectro 2. MAIN MENU 12:00:00 The LaMotte logo screen will appear for PROGRAMMED TESTS about 2 seconds. After initialization the %T/ABS MAIN MENU will appear.
Page 30 EDIT CLOCK STORE METHOD BEEPER ON/OFF CORRECTION WL LANGUAGE 6. Press *ENTER to select BT PRINTER. BT PRINTER CONNECT DISCONNECT SCAN FOR DEVICES SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 31 FIRST TIME PAIRING OF PRINTER AND SPECTRO To pair the Mobile Spin Printer to the SMART Spectro 2 for the ﬁ rst time, scroll to SCAN FOR DEVICES. If the printer and SMART Spectro 2 have already been paired, go to page 32.
Page 32 8. Press EXIT to return to SYSTEM SETUP. SYSTEM SETUP DARK CURRENT CALIBRATE WL BT PRINTER EDIT CLOCK STORE METHOD BEEPER ON/OFF CORRECTION WL LANGUAGE SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 33: Edit Clock
 EDIT CLOCK The clock information is used to time stamp the data points in the data logger. 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds. After PROGRAMMED TESTS initialization the MAIN MENU will appear.
Page 34 Note: Hours are in a 24 hour format. 00:13:10 14-04-2016 SET SECOND: EDIT CLOCK 6. Press to scroll to adjust the seconds to the correct time. 00:13:10 14-04-2016 SET SECOND: SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 35: Store Method
BT PRINTER EDIT CLOCK STORE METHOD BEEPER ON/OFF CORRECTION WL LANGUAGE  STORE METHOD 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds. After PROGRAMMED TESTS initialization the MAIN MENU will appear. %T/ABS...
Page 36 SYSTEM SETUP 4. Press to scroll to STORE METHOD. DARK CURRENT CALIBRATE WL BT PRINTER EDIT CLOCK STORE METHOD BEEPER ON/OFF CORRECTION WL LANGUAGE SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 37 SYSTEM SETUP and return to the SYSTEM SETUP MENU. DARK CURRENT CALIBRATE WL BT PRINTER EDIT CLOCK STORE METHOD BEEPER ON/OFF CORRECTION WL LANGUAGE SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 38: Beeper On/Off
 BEEPER ON/OFF If the beeper function is turned ON, the beeper will sound at each button push. The default beeper setting is ON. 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds. After PROGRAMMED TESTS initialization the MAIN MENU will appear.
Page 39 BEEPER ON/OFF CORRECTION WL LANGUAGE 5. Press *ENTER to select BEEPER ON/OFF. BEEPER ON/OFF BEEPER ON/OFF 6. Press to highligh ON or OFF. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 40: Correction Wl
CORRECTION WL LANGUAGE  CORRECTION WL The Correction WL feature should only be used by service personnel. It is used to calibrate the system and the wavelength. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 41: Language
 LANGUAGE There are two choices for language: English and Chinese. The default language is English. 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds. After PROGRAMMED TESTS initialization the MAIN MENU will appear.
Page 42 CORRECTION WL LANGUAGE 5. Press *ENTER to select LANGUAGE. LANGUAGE ENGLISH CHINESE LANGUAGE 6. Press to scroll to highlight ENGLISH or CHINESE. ENGLISH CHINESE SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 43 STORE METHOD BEEPER ON/OFF CORRECTION WL LANGUAGE  RESET TO DEFAULT The SMART Spectro 2 can be reset to the default settings. 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds. After PROGRAMMED TESTS initialization the MAIN MENU will appear.
Page 44 Default. Progress bars PROGRAMMED TESTS will show the reset progress of the system %T/ABS data (SD) and the customer data (UD) PC LINK SYSTEM SETUP SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 45: Initialization
When ON is pressed to turn the spectrophometer on the meter will automatically go through initialization and begin a 15 minute warm up. To skip the 15 minute warm up, press EXIT. After initialization, the main menu will be displayed. 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds.
Page 46: Introduction
PROGRAMMED TESTS  INTRODUCTION The PROGRAMMED TESTS mode is used to run all LaMotte pre-programmed tests and USER TESTS. This is also where USER TESTS and SEQUENCES are set-up and edited. 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds.
Page 47: Sequences Of Tests
Pressing the EXIT button while in a sequence menu will escape back to the PROGRAMMED TESTS menu. Pressing and holding the OFF button at any time will turn the SMART Spectro 2 oﬀ . SMART Spectro 2 Operator’s Manual 04.16...
Page 48: General Testing Procedures
 GENERAL TESTING PROCEDURES The following are some step by step examples of how to run tests from the PROGRAMMED TESTS menu. These test procedures are designed to be used with LaMotte SMART Spectro 2 reagent systems. TESTING WITH THE PROGRAMMED TESTS 1.
Page 49 1 ALKALINITY-UDV 2 ALUMINUM 3 AMMONIA-N L F 4 AMMONIA-N L S 5 AMMONIA-N H 6 ARSENIC 7 BIGUANIDE 8 BORON SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 50 T = 13.5%T If Store Method is automatic, STORE PRESS [ENTER] will not be displayed. A = 0.8707A C = 0.26 ppm STORE PRESS [ENTER] SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 51 1. Go to the PROGRAMMED TESTS menu. PROGRAMMED TESTS 535NM Press to scroll to EDIT. SEQUENCE 1 SEQUENCE 2 SEQUENCE 3 ALL TESTS EDIT SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 52: Adding Or Deleting Tests
535NM 1. Press to scroll to the sequence to be edited. EDIT SEQUENCE 1 EDIT SEQUENCE 2 EDIT SEQUENCE 3 EDIT USER TESTS SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 53 1 ALKALINITY-UDV 2 ALUMINUM 3 AMMONIA-N L F 4 AMMONIA-N L S 5 AMMONIA-N H 6 ARSENIC 7 BIGUANIDE 8 BORON SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 54 Press *ENTER to save the ﬁ rst test in EDIT SEQUENCE 3 535NM the sequence and return to editing the INSERT sequence. DELETE 2 ALUMINUM SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 55 EXIT to exit the EDIT SEQUENCE 3 menu and return to the EDIT menu. Press EXIT again to exit the EDIT menu. The meter will save any changes and go to the PROGRAMED TESTS menu. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 56 78 Phosphate L Aluminum EDIT SEQUENCE 3 535NM 3. Press to scroll to the test to be deleted. 78 Phosphate L Aluminum SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 57 TESTS menu. Press EXIT to exit the EDIT SEQUENCE 3 menu to the EDIT menu. Press EXIT to exit the EDIT menu. The meter will save any changes and go the PROGRAMMED TESTS menu. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 58: Edit User Tests
A User Test may be added for a reagent system for which no precalibrated test exists. A calibration of a LaMotte reagent system may also be entered. The calibration of a User Test can be changed at any time.
Page 59 103 USER TEST 3 104 USER TEST 4 105 USER TEST 5 106 USER TEST 6 107 USER TEST 7 108 USER TEST 8 SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 60: Naming The Test
It is before the letter A. DO NOT CHOOSE THE ! UNTIL THE NAMING OF THE TEST HAS BEEN COMPLETED. The terminator should be selected following the end of the name. It must be the last character selected. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 61 ! A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0 1 2 3 4 5 6 7 8 9 _ . SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 62: Selecting The Wavelength
User Test is now named H2O.  SELECTING THE WAVELENGTH 1. Scroll to SELECT WL. 102 H2O 535NM NAME THE TEST SELECT WL NEW CALIBRATION FORMAT RESULT SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 63 4. Press *ENTER to save the wavelength. The 102 H2O 465NM display will return to the USER TEST menu. NAME THE TEST SELECT WL NEW CALIBRATION FORMAT RESULT SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 64: Entering A New Calibration
NAME THE TEST calibration curve. The maximum number SELECT WL of data points is 8. NEW CALIBRATION FORMAT RESULT INPUT DATA NUMBERS = 4 SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 65 The display will indicate BLANKED. A highlighted box will appear on ! 0 1 2 3 4 5 6 7 8 9 . the character selection line. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 66 9. Press EXIT to return to the New Calibration NEW CALIBRATION 465NM menu. ENTER STD. 1 ENTER STD. 2 ENTER STD. 3 ENTER STD. 4 CALCULATE SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 67 0 ppm, 0 absorbance. This is a classical Beers Law calibration. If a one point calibration has been performed, the 1. DEGREE THRU.0 curve ﬁ t must be SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 68: Selecting The Numerical Format Of The Result
ﬁ gures than will be displayed. Menu choices of 0, 1, 2, or 3 decimal places will be given for the display. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 69 DECIMAL PLACES 465NM 3. Press to select the number of decimal places to be displayed. 0 PLACES 1 PLACES 2 PLACES 3 PLACES SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 70: Measuring In The %T/Abs Mode
105 USER TEST 5 106 USER TEST 6 107 USER TEST 7 108 USER TEST 8 MEASURING IN THE %T/ABS MODE 1. Press ON. The LaMotte logo screen MAIN MENU 12:00:00 will appear for about 2 seconds. After PROGRAMMED TESTS initialization the MAIN MENU will appear.
Page 71 4. Press *ENTER to select SELECT WL. %T/ABS 465NM SELECT WL SCAN BLANK SCAN SAMPLE TEST RESULT LIST END OF LIST PLEASE SELECT WL = 465 SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 72 SELECT WL For most %T/ABS tests, a clear, colorless SCAN BLANK blank should be used. SCAN SAMPLE TEST RESULT LIST END OF LIST SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 73 10. To view the test results, scroll to TEST %T/ABS 466NM RESULT LIST. SELECT WL SCAN BLANK SCAN SAMPLE TEST RESULT LIST END OF LIST SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 74 Stored data can be exported or printed with the Mobile Bluetooth Printer (Code 3-0066) by selecting PC Link from the MAIN MENU. To establish a connection with the printer go to SYSTEM SETUP and select BT PRINTER. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 75: Delete Data
LANGUAGE 3. Scroll to STORE METHOD. SYSTEM SETUP DARK CURRENT CALIBRATE WL BT PRINTER EDIT CLOCK STORE METHOD BEEPER ON/OFF CORRECTION WL LANGUAGE SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 76 AUTOMATIC MANUAL END OF LIST 6. Press and hold *ENTER for 8 seconds to clear the memory. WARNING: This will delete all of the saved data. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 77: Print Data
Then press *ENTER to print. PC LINK The SMART Spectro 2 may be interfaced with any Windows-based computer by using a LaMotte SMARTLink Program and USB Cable. The program will store test information and results in a database. To transfer data from the meter to a computer, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB Type A connector) into a USB port on a computer.
Page 78: Cleaning
PARTS OF THE METER.  LAMP REPLACEMENT AND REALIGNMENT The quartz halogen bulb in the SMART Spectro 2 has an approximate life of 1000 hours. If a test is performed and there is no response and the unit is receiving adequate power, the light bulb may need to be re-secured or replaced.
Page 79 Back view of the Lamp bracket installation lamp bracket guiding hole Lamp bracket mounting screw (2) Lamp-locking screw (2) Lamp Bracket Assembly (shown with lamp) SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 80: Halogen Lamp Replacement
Lamp-locking screw (2) 4. Insert the new lamp (12V/10W) into the lamp socket and scure the lamp with the lamp-locking screws. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 81 If the light beam is not focused on the entrance slit. Adjust the lamp bracket to bring the beam into focus on the slit. Tighten the bracket locking screws. The light beam from the lamp must be focused on the entrance slip. SMART Spectro 2 Operator’s Manual 04.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 82: Clock Battery
 CLOCK BATTERY (RAM BATTERY) The SMART Spectro 2 date/clock function is powered by a CR 1220 battery. This battery should be replaced about every 8 years. If the date or clock function stops or if you receive the message “Warning RAM Battery Low” you should replace the RAM battery as follows, or contact the LaMotte Technical Service Department by phone at 1-800-344-3100, fax 410-778-6394 or email tech@lamotte.com...
Page 83: Meter Disposal
BATTERY (optional) If the symbol BAT appears in the upper left corner of the display when using the battery pack, the battery needs to be charged. The SMART Spectro 2 will turn oﬀ if the battery power becomes too low.
Page 84: Helpful Hints
The power supply has an internal switch that handles both 110V and 220V input. STRAY LIGHT The SMART Spectro 2 should have no problems with stray light. Make sure that the sample compartment lid is always fully closed. ACCIDENTAL LOSS OF POWER...
Page 85 TEST PROCEDURES 2000-02-MN 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 86 SPECTROPHOTOMETER REAGENT SYSTEMS LIST Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA) or email at tech@lamotte.com for a current list of available calibrations and downloading instructions. # of Test Factor (Test #) Range (ppm) Test Method (# of Reagents)
Page 87 Copper-DDC (32) 0.00–6.00 0.05 Diethyldithiocarbamate (1) Copper-UDV (33) 0.00–4.00 0.20 Bicinchoninic Acid (1)† Cyanide (35) 0.00-0.50 0.50 Pyridine-Barbituric Acid (5) Cyanuric Acid (36) 0–200 Melamine (1) Cyanuric Acid-UDV (37) 0–150 Melamine (1)† DEHA (037) 0.000–0.700 0.005 Iron Reduction (3) See Oxygen Scavengers Dissolved Oxygen (39) 0.0–12.0 0.25...
Page 88 Nitrogen, Total (62) 0-25 mg/L 2 mg/L Chromotropic Acid/Digestion (6)* Oxygen Scanvengers various various Oxygen Reduction (3) Ozone-Low Range (071) 0.00–0.40 0.02 Indigo Trisulfonate (3) Ozone-High Range (072) 0.00–1.50 0.05 Indigo Trisulfonate (3) pH-Chlorophenol Red (073) 5.0-7.0 – Chlorophenol Red (3) pH-Phenol Red (074) 6.6–8.4 –...
Page 89 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 90 Samples should be analyzed as soon as possible after PRESERVATION: collection. Sample may be refrigerated for 24 hours. INTERFERENCES: Quats and poly quats at high concentrations will interfere. ALKALINITY, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 91 5 days if humidity is greater than 50%. The Foil Storage Bag contains a dessicant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced. SMART Spectro 2 Test Procedures 05.02.16 ALKALINITY, UDV GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 92 ALKALINITY, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 93 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 94 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. ALUMINUM SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 95 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 96 There are few interferences in most natural waters. High concentrations of reducing agents, such as hydrazine, react with the chlorine donor and can result in negative interferences. Color and turbidity can also interfere. AMMONIA-NITROGEN, Low Range SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 97 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 05.02.16 AMMONIA-NITROGEN, Low Range GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 98 SCAN SAMPLE. Record result. 11. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. AMMONIA-NITROGEN, Low Range SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 99 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. To determine the percentage of Ammonia-Nitrogen that is unionized and ionized, consult the Appendix. SMART Spectro 2 Test Procedures 05.02.16 AMMONIA-NITROGEN, Low Range GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 100 AMMONIA-NITROGEN, Low Range SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 101 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 102 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. To determine the percentage of Ammonia-Nitrogen that is unionized and ionized, consult the Appendix. AMMONIA-NITROGEN, High Range SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 103 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 104 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. BIGUANIDE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 105 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 106 19. Press and hold OFF button to turn the spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. BORON SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 107 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 108 Invert slowly 5 times. Solution will turn pink if bromine is present. Insert tube into chamber, close lid and select SCAN SAMPLE. Press and hold OFF button to exit to a previous menu or make another menu selection. BROMINE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 109 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 110 Iodine and chlorine can also interfere, but these are not normally present unless they have been added as sanitizers. BROMINE, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 111 50% and within 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced. SMART Spectro 2 Test Procedures 05.02.16 BROMINE, UDV GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 112 BROMINE, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 113 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 114 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. CADMIUM SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 115 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 116 The reagent system is temperature sensitive. The calibration is for 25ºC. If sample is at 30ºC, multiply resulting ppm by 1.1. If the sample is at 20ºC, multiply resulting ppm by 0.9. CHLORINE, TESTAB SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 117 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 118 Iodine and bromine can give a positive interference, but these are not normally present unless they have been added as sanitizers. CHLORINE, LIQUID SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 119 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 05.02.16 CHLORINE, LIQUID GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 120 CHLORINE, LIQUID SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 121 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 122 Iodine and bromine can give a positive interference, but these are not normally present unless they have been added as sanitizers. CHLORINE, DPD TABLET SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 123 12. Press and hold the OFF button to turn oﬀ the spectrophotometer or press the EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 CHLORINE, DPD TABLET GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 124 CHLORINE, DPD TABLET SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 125 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 126 Iodine and bromine can give a positive interference, but these are not normally present unless they have been added as sanitizers. CHLORINE-FREE, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 127 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced. SMART Spectro 2 Test Procedures 05.02.16 CHLORINE-FREE, UDV GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 128 CHLORINE-FREE, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 129 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 130 Iodine and bromine can give a positive interference, but these are not normally present unless they have been added as sanitizers. CHLORINE-TOTAL, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 131 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced. SMART Spectro 2 Test Procedures 05.02.16 CHLORINE-TOTAL, UDV GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 132 CHLORINE-TOTAL, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 133 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 134 Immediately Insert tube into chamber, close lid and select SCAN SAMPLE. 10. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. CHLORINE DIOXIDE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 135 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 136 Highly buﬀ ered waters may give poor results and require a more careful pH adjustment. Before adding *Chromium Reagent Powder, adjust pH of sample to pH 3-4. CHROMIUM, HEXAVALENT SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 137 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 138 INTERFERENCES: High concentrations of mercurous and mercuric ions may interfere. CHROMIUM, HEXAVALENT, TRIVALENT & TOTAL SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 139 Remove ﬂ ask from heat. Cool sample under running water. This is the digested sample. Pour digested sample into clean graduated cylinder (0418). Dilute to the 50 mL line SMART Spectro 2 Test Procedures 05.02.16 CHROMIUM, HEXAVALENT, TRIVALENT & TOTAL GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 140 TRIVALENT CHROMIUM PROCEDURE Subtract hexavalent chromium from total chromium. Record as ppm trivalent chromium. Trivalent Chromium = Total Chromium – Hexavalent Chromium CHROMIUM, HEXAVALENT, TRIVALENT & TOTAL SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 141 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 142 To convert results to ppm chromate (CrO ), multiply by 2.23. To convert result to ppm sodium chromate (Na ) multiply by 3.12. CHROMIUM, TESTAB SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 143 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 144 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. COBALT SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 145 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 146 Other reduced inorganic compounds are stoichiometrically oxidized, causing a positive interference. Corrections can be made for these compounds based upon their stoichiometry and concentrations. COD, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 147 15. Wipe the blank tube with a damp towel to remove ﬁ ngerprints and smudges. Wipe with a dry towel. 16. Insert reagent blank tube into chamber. Align the center of the LaMotte logo on the tube with the arrow shaped mark molded into the housing at the front edge of the light chamber.
Page 148 15 minute intervals while the vials are still hot until the reading remains unchanged. The vials should be cooled to room temperature before the ﬁ nal measurement is taken. COD, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 149 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 150 Other reduced inorganic compounds are stoichiometrically oxidized, causing a positive interference. Corrections can be made for these compounds based upon their stoichiometry and concentrations. COD, STANDARD RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 151 15. Scroll to and select 26 COD SR 0-1500 from menu. 16. Insert reagent blank tube into chamber. Align the center of the LaMotte logo on the tube with the arrow shaped mark molded into the housing at the front edge of the light chamber.
Page 152 15 minute intervals while the vials are still hot until the reading remains unchanged. The vials should be cooled to room temperature before the ﬁ nal measurement is taken. COD, STANDARD RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 153 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 154 Other reduced inorganic compounds are stoichiometrically oxidized, causing a positive interference. Corrections can be made for these compounds based upon their stoichiometry and concentrations. COD, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 155 15. Scroll to and select 27 COD HR 0-15000 from menu. 16. Insert reagent blank tube into chamber. Align the center of the LaMotte logo on the tube with the arrow shaped mark molded into the housing at the front edge of the light chamber.
Page 156 15 minute intervals while the vials are still hot until the reading remains unchanged. The vials should be cooled to room temperature before the ﬁ nal measurement is taken. COD, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 157 Collect all samples in clean glassware. Determine color as PRESERVATION: soon as possible to avoid biological or chemical changes that could occur in the sample during storage. INTERFERENCES: Turbidity will interfere. Filter before testing. SMART Spectro 2 Test Procedures 05.02.16 COLOR GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 158 10. Press and hold OFF button to turn the spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. COLOR SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 159 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 160 SCAN SAMPLE. Record result. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. COPPER, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 161 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 162 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. The reaction may stain the tubes. Scrub tubes thoroughly after each use. COPPER, CUPRIZONE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 163 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 164 Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. NOTE: The reaction may stain the tubes. Scrub the tubes thoroughly after each use. COPPER, DDC SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 165 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 166 “plating out”. However, a correction must be made to bring the reaction into the optimum pH range. INTERFERENCES: High concentrations of oxidizing agents, calcium, and magnesium interfere. Silver can also interfere. COPPER, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 167 5 days if humidity is greater than 50%. The Foil Storage Bag contains a dessicant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced. SMART Spectro 2 Test Procedures 05.02.16 COPPER, UDV GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 168 COPPER, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 169 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 170 Thiocyanate and cyanogen chloride both react as cyanide in this test and will give a positive interference. Color and turbidity can also interfere. CYANIDE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 171 SCAN SAMPLE. Record result. 13. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 CYANIDE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 172 CYANIDE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 173 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 174 10. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. NOTE: For the most accurate results, the sample and reagents should be at 25 ±4°C. CYANURIC ACID SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 176 No known interference from compounds normally found in pool water. Temperature of the sample should be maintained between 70°F and 80°F for best results. Check for stray light interference (see page 16). CYANURIC ACID, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 177 UDVs stored in the bag should be used within 10 days if the humidity is less than 50% and within 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack SMART Spectro 2 Test Procedures 05.02.16 CYANURIC ACID, UDV GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 178 CYANURIC ACID, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 179: Dissolved Oxygen
*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 180 Steps 1 through 4 below describe proper sampling technique in shallow water. For sample collection at depths beyond arm’s reach, special water sampling apparatus is required (e.g. the LaMotte Water Sampling Chamber, Code 1060; Model JT-1 Water Samplers, Code 1077; Water Sampling Outﬁ t, Code 3103; or Water Sampling Bottle, Code 3-0026).
Page 181 SCAN SAMPLE. Record result. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 DISSOLVED OXYGEN GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 182 DISSOLVED OXYGEN SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 183 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 184 –2 200 mg/L Color and turbidity must be removed or compensated for in the procedure. Temperature should be maintained within 5°C of room temperature. FLUORIDE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 185 10. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result. 11. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 FLUORIDE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 186 FLUORIDE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 187 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 188 100 mL of sample. However, the added acid will have to be neutralized with NaOH before testing. INTERFERENCES: Heavy metals will interfere. HARDNESS, TOTAL-UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 189 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced. SMART Spectro 2 Test Procedures 05.02.16 HARDNESS, TOTAL-UDV GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 190 HARDNESS, TOTAL-UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 191 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 192 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. HYDRAZINE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 193 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 194 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. For the most accurate results, the sample and reagents should be at 25±4°C. HYDROGEN PEROXIDE, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 195 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 196 The likelihood of other oxidizing compounds interfering with this method is eliminated by the presence of hydrogen peroxide. Manganese may interfere and should be removed before analysis HYDROGEN PEROXIDE, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 197 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. For the most accurate results, the sample and reagents should be at 25 ±4°C. SMART Spectro 2 Test Procedures 05.02.16 HYDROGEN PEROXIDE, HIGH RANGE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 198 HYDROGEN PEROXIDE, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 199 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 200 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. For the most accurate results, the sample and reagents should be at 25 ±4°C. HYDROGEN PEROXIDE, SHOCK SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 201 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 202 Chlorine and bromine can give a positive interference, but these are not normally present unless they have been added as sanitizers. IODINE, DPD TABLET SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 203 Insert tube into chamber, close lid and select SCAN SAMPLE. Record result. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 IODINE, DPD TABLET GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 204 IODINE, DPD TABLET SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 205 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 206 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. IRON, BIPYRIDYL SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 207 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 208 10 times that of iron; cobalt and copper in excess of 5 mg/L, and nickel in excess of 2 mg/L. Bismuth, cadmium, mercury, , and silver precipitate phenanthroline. IRON, I,I0-PHENANTHROLINE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 209 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 05.02.16 IRON, I,I0-PHENANTHROLINE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 210 IRON, I,I0-PHENANTHROLINE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 211 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 212 Samples should be analyzed as soon as possible. INTERFERENCES: Strong oxidizing agents interfere, as well as copper and cobalt in excess of 5.0 ppm. IRON, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 213 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced. SMART Spectro 2 Test Procedures 05.02.16 IRON, UDV GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 214 IRON, UDV SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 215 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 216 INTERFERENCES: Calcium greater than 100 ppm (250 ppm CaCO ) will interfere. Low concentrations of cerium, iron, manganese, magnesium, sulfur, tin, and EDTA will also interfere. LEAD SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 217 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 05.02.16 LEAD GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 218 LEAD SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 219 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 220 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. MANGANESE, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 221 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 222 SCAN SAMPLE. Record result. 10. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. MANGANESE, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 223 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 224 Subtract the reagent blank results from all subsequent test results of unknown samples. It is recommended that a reagent blank be determined each time *TMK Indicator is prepared. MERCURY SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 225 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 226 10. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result. 11. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. MOLYBDENUM SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 227 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 228 14. Insert test sample into chamber, close lid and select SCAN SAMPLE. Record result. 15. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. NICKEL SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 229 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 230 Nitrite interferes at all concentrations. Strong oxidizing and reducing substances interfere. Low results might be obtained for samples that contain high concentrations of copper and iron. NITRATE, TABLET SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 231 Subtract the reagent blank from all subsequent test results of unknown samples. To convert nitrate (NO ) results to nitrate-nitrogen (NO -N), divide by 4.4. SMART Spectro 2 Test Procedures 05.02.16 NITRATE, TABLET GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 232 NITRATE, TABLET SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 233 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 234 Nitrite interferes at all levels. Strong oxidizing and reducing substances interfere. Low results might be obtained for samples that contain high concentrations of iron and copper. NITRATE-NITROGEN, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 235 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. To convert Nitrate Nitrogen (NO –N) results to ppm Nitrate (NO ), multiply by 4.4. SMART Spectro 2 Test Procedures 05.02.16 NITRATE-NITROGEN, LOW RANGE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 236 NITRATE-NITROGEN, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 237 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 238 NOTE: To convert nitrite-nitrogen (NO –N) results to ppm nitrite (NO ), multiply results by 3.3. NITRITE-NITROGEN, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 239 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 240 Subtract the reagent blank from all subsequent test results of unknown samples. To convert nitrite (NO ) results to nitrite-nitrogen (NO –N), divide results by 3.3. NITRITE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 241 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 242 5.0 N sodium hydroxide before analyzing. INTERFERENCES: Bromide (>60 ppm) and chloride (>1000 ppm) will have a positive interference. NITROGEN, TOTAL SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 243 Invert the tube and wait for the air bubble to ﬂ ow to the bottom of the tube. Turn the tube upright and wait for the air bubble to return to the top of the tube. This is one SMART Spectro 2 Test Procedures 05.02.16 NITROGEN, TOTAL...
Page 244 NOTE: For greater accuracy, use laboratory grade pipets. To order reagent reﬁ lls, order code R-4026. NITROGEN, TOTAL SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 245 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 246 Other oxygen scavengers, such as DEHA, carbohydrazide, erythorbic acid, hydroquinone and methylethylketoxime will interfere. Stray light and substances which complex iron or reduce ferric iron will also interfere. OXYGEN SCAVENGERS SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 247 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 05.02.16 OXYGEN SCAVENGERS GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 248 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. OXYGEN SCAVENGERS SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 249 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 05.02.16 OXYGEN SCAVENGERS GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 250 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. OXYGEN SCAVENGERS SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 251 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 05.02.16 OXYGEN SCAVENGERS GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 252 OXYGEN SCAVENGERS SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 253 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 254 14. Insert reacted Sample Tube into chamber, close lid and select SCAN SAMPLE. Record result. 15. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. OZONE SMART Spectro 2 Test Procedures 07.05.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 255 72 Ozone HR) from TESTING MENU. 12. Scroll to and select 72 Ozone HR from menu. 13. Insert the Reagent Blank tube into chamber, close lid and select SCAN BLANK. SMART Spectro 2 Test Procedures 07.05.16 OZONE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 256 NOTE: HR Reagent must be made fresh each week. If reagent is refrigerated, it may be kept up to 3 weeks. OZONE SMART Spectro 2 Test Procedures 07.05.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 257 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 258 Indicator SMART Spectro Test Range Chlorphenol Red 5.0–7.0 74 pH CPR Phenol Red 6.6–8.4 75 pH PR Thymol Blue 8.0–9.5 76 pH TB SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 259 Insert tube into chamber, close lid and select SCAN SAMPLE. Record result. 10. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 260 SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 261 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 262 10. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result. 11. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. PHENOL SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 263 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 264 (less than 50 mg/L). This interference is overcome by spiking samples with a minimum of 50 mg/L of sodium chloride. PHOSPHATE, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 265 SCAN SAMPLE. Record result. 10. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 PHOSPHATE, LOW RANGE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 266 PHOSPHATE, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 267 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 268 SAMPLE. Record result. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. PHOSPHATE, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 269 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 270 (>10 ppm), Iron (>100 ppm), Nickel (>300 ppm), silica (>50 ppm), silicate (>10 ppm), sulﬁ de (>90 ppm) and zinc (>80 ppm) will interfere. PHOSPHORUS, TOTAL, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 271 18. Press and hold OFF button to turn the spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. NOTE: For greater accuracy, use laboratory grade pipets. SMART Spectro 2 Test Procedures 05.02.16 PHOSPHORUS, TOTAL, LOW RANGE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 272 PHOSPHORUS, TOTAL, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 273 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 274 Ferrous iron concentrations above 100 ppm will interfere. NOTE: For greater accuracy, use laboratory grade pipets. To order reagent reﬁ lls, order code R-4025. PHOSPHORUS, TOTAL, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 275 Total Phosphorus in mg/L PO 16. Press and hold OFF button to turn the spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 PHOSPHORUS, TOTAL, HIGH RANGE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 276 PHOSPHORUS, TOTAL, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 277 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 278 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. For the most accurate results, the sample and reagents should be at 25±4°C. POTASSIUM SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 279 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 280 The blank value is subtracted from the sample concentrations. SILICA, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 281 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 05.02.16 SILICA, LOW RANGE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 282 SILICA, LOW RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 283 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 284 INTERFERENCES: Sulﬁ des and large amounts of iron interfere. Color and turbidity may be removed by standardizing the instrument with the original water sample. SILICA, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com...
Page 285 NOTE: To extend the range to 100 ppm, perform a 2:1 dilution of water sample, with silica- free water. Perform test and multiply result by 2. SMART Spectro 2 Test Procedures 05.02.16 SILICA, HIGH RANGE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 286 SILICA, HIGH RANGE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 287 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 288 A white ﬁ lm is deposited on the inside of test tubes as a result of the sulfate test. Thoroughly clean and rinse test tubes after each test. For the most accurate results, samples and reactions should be at 25±4°C. SULFATE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 289 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 290 High concentrations of sulﬁ de will inhibit the reaction, but dilution of the sample prior to analysis eliminates this problem. SULFIDE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 291 10. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result. 11. Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. SMART Spectro 2 Test Procedures 05.02.16 SULFIDE GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 292 SULFIDE SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 293 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 294 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SURFACTANTS SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 295 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 296 It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. For the most accurate results, the sample and reagents should be at 20±2°C. TANNIN SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 297 Press and hold OFF button to turn spectrophotometer oﬀ or press EXIT button to exit to a previous menu or make another menu selection. NOTE: For the most accurate results, the sample should be at 25±4°C. SMART Spectro 2 Test Procedures 05.02.16 TURBIDITY GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 298 The concentration of this stock solution is 400 FTU. This stock solution is stable for one month. Dilutions from this stock solution should be prepared fresh daily with low turbidity water. TURBIDITY SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 299 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures.
Page 300 The following ions interfere in concentrations greater than those listed. MG/L MG/L Cd(II) Cr(III) Al (III) Ni(II) Mn (II) Co (II) Fe (III) (II) Fe (II) ZINC SMART Spectro 2 Test Procedures 07.05.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 301 Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained. SMART Spectro 2 Test Procedures 07.05.16 ZINC GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com...
Page 302 ZINC SMART Spectro 2 Test Procedures 07.05.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!
Page 303 8.87 14.96 12.40 Freshwater data from Trussel (1972). Seawater values from Bower and Bidwell (1978). Salinity for Seawater values = 34% at an ionic strength of 0.701m. SMART Spectro 2 Test Procedures 07.05.16 APPENDIX GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www.
Page 304 1 ppm Total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized Ammonia- Nitrogen. Total Ammonia-Nitrogen 1.0000 ppm Unionized Ammonia-Nitrogen 0.1118 ppm Ionized Ammonia-Nitrogen 0.8882 ppm APPENDIX SMART Spectro 2 Test Procedures 05.02.16 GlobalScientificSupply GLOBAL SCIENTIFIC SUPPLY info@GlobalScientificSupply.com www. .com The Laboratory Supply Company!

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