Related Manuals for LaMotte SMART Spectro
Summary of Contents for LaMotte SMART Spectro
- Page 1 SMART Spectro ••••••••••••••••••••••••••••••••••••••••••••••• Spectrophotometer OPERATOR’S MANUAL L M o tt v2.2 • Printed-04.08 2000-MN...
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Page 3: Table Of Contents
Preparing Dilute Standard Solutions················································ 16 Standard Additions ··········································································16 Sample Dilution Techniques & Volumetric Measurements ············ 17 Interferences ····················································································18 Stray Light Interference ···································································18 OPERATION OF THE SMART SPECTRO SPECTROPHOTOMETER Overview ·························································································19 Power Supply ····················································································19 Components ····················································································20 Quick Start ······················································································21-22 GENERAL OPERATING PROCEDURES The Keypad ······················································································23... - Page 4 ENERGY MODE ········································································································45 STORE METHOD ········································································································45 TEST MODE ········································································································46 BATTERY OPERATION Charging the Batteries ·····································································46 Running the SMART Spectro Using Batteries ································ 46 MAINTENANCE Cleaning ···························································································47 Light Bulb ························································································47 Clock Battery ···················································································47 Meter Disposal··················································································47 TROUBLESHOOTING GUIDE Error Messages ·················································································47-48 Helpful Hints ···················································································48 SMART REAGENT SYSTEMS Available Test Kits ············································································49-51...
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Page 5: General Information
Failure to do so could result in personal injury or damage to the equipment. The SMART Spectro should not be stored or used in a wet or corrosive environment. Care should be taken to prevent water or reagent chemicals from wet spectrophotometer tubes from entering the SMART Spectro chamber. -
Page 6: Limited Warranty
(1) years from original purchase date. In the event that a defect is found during the warranty time frame, LaMotte Company agrees that it will be repaired or replaced without charge except for the transporation costs. -
Page 7: Statistical And Technical Definitions
This is not a useful measure of the performance of a test method. 5. Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta, American Laboratory, Sept 2003, P . 34. SMART SPECTRO SPECTROPHOTOMETER 05.04... -
Page 8: Contents And Accessories
Sensitivity: Sensitivity is the resolution based on how this term is used in LaMotte catalogs. This term is not listed in any of the references. Sometimes it is used for detection limit. It is a confusing term and should be avoided. -
Page 9: Epa Compliance
EPA COMPLIANCE The SMART Spectro is an EPA-Accepted instrument. EPA-Accepted means that the instrument meets the requirements for instrumentation as found in test procedures that are approved for the National Primary Drinking Water Regulations (NPDWR) or National Pollutant Discharge Elimination System (NPDES) compliance monitoring programs. -
Page 10: Chemical Testing
For dissolved oxygen studies, or for tests requiring small sample sizes, a Water Sampler (LaMotte Code 1060) will serve as a subsurface or in-depth sampler. This weighted device is lowered to the sampling depth and allowed to rest at... -
Page 11: Filtration
A Snap-Plunger Water Sampler (LaMotte Code 1077) is another “in-depth” sampling device which is designed to collect large samples which can be used for a multitude of tests. Basically, this collection apparatus is a hollow cylinder with a spring loaded plunger attached to each end. -
Page 12: An Introduction To Colorimetric Analysis & Spectroscopy
Most test substances in water are colorless and undetectable to the human eye. To test for their presence we must find a way to “see” them. The LaMotte SMART Spectro can be used to measure any test substance that is itself colored or can be reacted to produce a color. -
Page 13: Reagent Blank
Handle the tubes carefully, making sure the bottom half of the tube is not handled. LaMotte Company makes every effort to provide high quality spectrophoto- meter tubes. However, wall thicknesses and diameter of tubes may still vary slightly. -
Page 14: Calibration Curves
Absorbance or %T, and finding the corresponding concentration from the curve. The linear range of the reagent system can be determined and this information can be used to input a User Test into the SMART Spectro (see EDIT USER TESTS, page 33). - Page 15 A sample of each type of graph appears below: CALIBRATION CURVE CALIBRATION CURVE Absorbance vs. Concentration %T vs. Concentration 1 2 3 4 5 6 8 9 10 1 2 3 4 5 6 8 9 10 Concentration in ppm Concentration in ppm SMART SPECTRO SPECTROPHOTOMETER 05.04...
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Page 16: Preparing Dilute Standard Solutions
The procedure can be repeated with larger and larger “spikes.” If the determined concentrations do not equal the concentration of the sample plus that added with the “spike”, then an interference may exist. SMART SPECTRO SPECTROPHOTOMETER 05.04... -
Page 17: Sample Dilution Techniques & Volumetric Measurements
0.3 + 0.5 = 0.8 ppm iron. (Note: any error due to the increased volume from the “spike” is negligible). LaMotte offers a line of calibration standards which can be used to generate calibration curves and perform standard additions. SAMPLE DILUTION TECHNIQUES &... -
Page 18: Interferences
INTERFERENCES LaMotte reagent systems are designed to minimize most common interferences. Each individual test instruction discusses interferences unique to that test. Be aware of possible interferences in the water being tested. The reagent systems also contain buffers to adjust the water sample to the ideal pH for the reaction. -
Page 19: Operation Of The Smart Spectro Spectrophotometer
The test library consists of 100 LaMotte tests (not all 100 may be available at present) and 25 “User Tests”. The spectrophotometer is also capable of running %T/Absorbance tests over the entire wavelength range of 350 - 1000 nm. -
Page 20: Components
COMPONENTS Figure 1 shows a diagram of the SMART Spectro and the components. RS232 adapter serial socket port 10mm square cell in Adapter 25mm round cell in Universal Adapter Display LAMOTTE SMART SPECTRO Chamber PRESS ENTER Scrolling Scrolling arrows for... -
Page 21: Quick Start
QUICK START Some quick instructions to get into testing. 1. Press ON to turn on the SMART Spectro. LAMOTTE SMART The WELCOME screen will appear for about 2 seconds. Version 1.0 May 2000 MAIN MENU 12:45 2. The MAIN MENU screen will appear automatically. - Page 22 10. Insert reacted sample. Press ENTER/Q to 15 Chlorine 16.5%T 0.7834 A scan sample. 1.28 PPM PRINT PRESS ENTER After obtaining test results, scroll with t or s, and make another selection with ENTER/Q. Press EXIT to escape to previous menus. SMART SPECTRO SPECTROPHOTOMETER 05.04...
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Page 23: General Operating Procedures
GENERAL OPERATING PROCEDURES The operation of the SMART Spectro is controlled by a microprocessor. The microprocessor is programmed with menu driven software. A menu is a list of choices. This allows a selection of various tasks for the spectrophotometer to perform, such as, scan blank, scan sample, and edit test sequences. -
Page 24: The Display & The Menus
This allows a rapid exit from MAIN MENU EXIT an inner menu to the by repeatedly pushing the button. Pushing at any time will turn the spectrophotometer off. SMART SPECTRO SPECTROPHOTOMETER 05.04... -
Page 25: Calibration
The Calibrate Wavelength (CALIBRATE WL) mode is used to establish or re-establish the accuracy of the wavelength selection process. Normally, the Calibrate Wavelength procedure should be run after the SMART Spectro is turned ON and allowed to warm up for 15 minutes or if operating conditions (temperature, humidity, etc.) change significantly. -
Page 26: Programmed Tests
Main Menu. PROGRAMMED TESTS INTRODUCTION The PROGRAMMED TESTS mode is used to run all LaMotte pre-programmed tests and USER TESTS. This is also where USER TESTS and SEQUENCES are set-up and edited. Press ON to turn on the SMART Spectro. -
Page 27: Sequences Of Tests
SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3 are alterable sequences. They may be edited using the EDIT function mode. Any of the LaMotte pre-programmed tests or User Tests may be placed in these sequences in whatever testing order that is preferred. Some examples of typical sequences are given below. -
Page 28: General Testing Procedures
The following are some step by step examples of how to run tests from the PROGRAMMED TESTS menu. These test procedures are designed to be used with LaMotte SMART Spectro reagent systems. TESTING WITH THE LaMOTTE PROGRAMMED TESTS Scroll to ALL TESTS with the t button in the PROGRAMMED TESTS PROGRAMMED TESTS menu. - Page 29 SCAN BLANK and then scanning another blank. Press the EXIT button to escape back to the 1 Aluminum PROGRAMMED TESTS menu, if no more samples SCAN BLANK are to be scanned for this test. * SCAN SAMPLE END 535NM SMART SPECTRO SPECTROPHOTOMETER 05.04...
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Page 30: Setup & Edit Sequences & User Tests
* is to the left of SEQUENCE 3 EDIT. Press the ENTER/Q button ALL TESTS * EDIT The EDIT menu appears. EDIT * EDIT SEQUENCE 1 EDIT SEQUENCE 2 EDIT SEQUENCE 3 SMART SPECTRO SPECTROPHOTOMETER 05.04... -
Page 31: Adding Or Deleting Tests
78 Phosphate L from the list and 77 Phenol insert it before END OF LIST. The display will * 78 Phosphate L automatically return to EDIT SEQUENCES 79 Phosphate H menu. END OF LIST Continued on next page... SMART SPECTRO SPECTROPHOTOMETER 05.04... - Page 32 EDIT SEQUENCE 3 menu and return to the EDIT menu. Press the EXIT button to exit the EDIT menu. EDIT The SMART Spectro will automatically save any * EDIT SEQUENCE 1 changes and go to the PROGRAMMED TESTS EDIT SEQUENCE 2 menu.
- Page 33 EDIT menu. END OF LIST Press the EXIT button to exit the EDIT menu. EDIT * EDIT SEQUENCE 1 The SMART Spectro will automatically save any changes and go to the PROGRAMMED TESTS EDIT SEQUENCE 2 menu. EDIT SEQUENCE 3...
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Page 34: Edit User Tests
EDIT USER TESTS If a test other than the LaMotte programmed tests is performed regularly, a calibration for it may be entered in one of the 25 User Tests. These tests are originally named “User Test 1 - 25". It will be possible to rename the test, select a wavelength, enter a new calibration and select the number of decimal places used to display the results. - Page 35 Any menu choice can be END OF LIST edited at any time by selecting it. The normal procedure would be to start with NAME THE TEST, then SELECT WAVELENGTH, then NEW CALIBRATION, and then FORMAT RESULT. SMART SPECTRO SPECTROPHOTOMETER 05.04...
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Page 36: Naming The Test
END OF LIST name. The display will return to the KEEP CHANGE menu. Select KEEP to save the name and return to 101 H2O menu. Note that test 101 is named H2O. Continued on next page... SMART SPECTRO SPECTROPHOTOMETER 05.04... -
Page 37: Selecting The Wavelength
Use s and t to scroll to the appropriate SELECT WAVELENGTH WAVELENGTH. 600 NM SET PRESS ENTER Press ENTER/Q to select the wavelength. 101 H2O Display goes back to 101 H2O screen. * NAME THE TEST SELECT WAVELENGTH NEW CALIBRATION FORMAT RESULT SMART SPECTRO SPECTROPHOTOMETER 05.04... -
Page 38: Entering A New Calibration
The menu will indicate the Spectro is blanking. BLANKING STD.1= !123456789. ENTER STD.1 When BLANKING is finished the display will BLANKED indicate BLANKED and a blinking cursor will appear on the character selection line. * STD.1= !0123456789. Continued on next page ... SMART SPECTRO SPECTROPHOTOMETER 05.04... - Page 39 When inputting 7 standards, ignore request for ENTER STD. 8. NEW CALIBRATION When finished inputting standards scroll down (in the NEW CALIBRATION menu) to the ENTER STD.7 CALCULATE selection. Select CALCULATE. ENTER STD.8 * CALCULATE Continued on next page... SMART SPECTRO SPECTROPHOTOMETER 05.04...
- Page 40 101 H2O DEGREES menu. Press EXIT button again to * NAME THE TEST escape back to Input Data Numbers menu. SELECT WAVELENGTH Press EXIT button to escape back to 101 H20 NEW CALIBRATION menu. FORMAT RESULT SMART SPECTRO SPECTROPHOTOMETER 05.04...
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Page 41: Selecting The Numerical Format Of The Result
The 101 H2O menu will appear. NAME THE TEST Since no more editing needs to be done press SELECT WAVELENGTH EXIT button to escape to EDIT USER TESTS NEW CALIBRATION menu. Continued on next page ... SMART SPECTRO SPECTROPHOTOMETER 05.04... - Page 42 NOTE: Test 101 was USER TEST 1 and now is H2O. It is still a USER TEST because its calibration can be changed but it has a different name. PROGRAMMED TESTS * SEQUENCE 1 SEQUENCE 2 SEQUENCE 3 ALL TESTS EDIT END OF LIST SMART SPECTRO SPECTROPHOTOMETER 05.04...
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Page 43: Measuring In The %T/Abs Mode
Push ENTER/Q button to select that wavelength . (Here 530 is selected as an example.) WL=530 The SMART Spectro is ready to scan. %T/ABS 530NM Insert blank into sample chamber and push ENTER/Q button to scan blank. SELECT WL... - Page 44 If no more samples are to be scanned, press EXIT %T/ABS 530NM to return to the MAIN MENU or press the OFF SELECT WAVELENGTH button to turn off the spectrophotometer. SCAN BLANK * SCAN SAMPLE SMART SPECTRO SPECTROPHOTOMETER 05.04...
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Page 45: Pc Link
PC LINK The SMART Spectro may be interfaced with any Windows-based program by using the LaMotte SMARTLink2 Program and Interface Cable (Code 1912-3 [3.5 disk] or 1912-CD [compact disk]). The program stores customer information and test data in a database. It can be used to download data stored in the SMART Spectro datalogger for each test site. -
Page 46: Test Mode
BATTERY OPERATION The SMART Spectro can be run using battery power. The battery pack consists of a rechargeable Ni-metal hydride battery pack. The battery pack is not included in the standard meter package and must be purchased as an accessory (Code 2000-BP). -
Page 47: Maintenance
With the meter power ON check the rear lamp access panel to see if any light can be observed through the vents. If the lamp is not ON please contact LaMotte Technical Services Department by phone at 800-344-3100 or 410-778-3100, fax 410-778-6394, or e-mail LaMotte.com for assistance. -
Page 48: Troubleshooting Guide
BATTERY (optional) If the symbol BAT appears in the upper left corner of the display when using the battery pack, the battery needs to be charged. The SMART Spectro will turn off if the battery power becomes too low. ERROR 1 TROUBLE WITH FILTER There is a problem with filter positioning. -
Page 49: Helpful Hints
The power supply has an internal switch that handles both 110V and 220V input. STRAY LIGHT The SMART Spectro should have no problems with stray light. Make sure that the sample compartment lid is always fully closed. ACCIDENTAL LOSS OF POWER...