Macherey-Nagel NucleoSpin 740727.10 User Manual page 2

Plasmid DNA purification
Protocol-at-a-glance (Rev. 01)
Cultivate
1
and harvest bacterial
cells
Cell lysis
2
3 Clarification of the lysate
4 Bind DNA
Wash and dry silica
5
membrane
6 Elute DNA
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NucleoSpin
®
Plasmid EasyPure
12,000 x g,
150 μL Buffer A1
250 μL Buffer A2
RT, up to 2 min
350 μL Buffer A3
> 12,000 x g,
3 min
Load supernatant
1,000–2,000 x g,
450 μL Buffer AQ
> 12,000 x g,
1 min
50 μL Buffer AE
RT, 1 min
> 12,000 x g,
1 min
30 s
30 s