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1.2
Reagents, consumables, and equipment to be supplied
by user
Reagents
• 96–100 % ethanol
Consumables
• 1.5 mL microcentrifuge tubes for sample lysis and DNA elution
• Disposable pipette tips
Equipment
• Manual pipettors
• Centrifuge for microcentrifuge tubes
• Vortex mixer
• Personal protection equipment (lab coat, gloves, goggles)
1.3
About this user manual
It is strongly recommended reading the detailed protocol sections of this user manual
if the NucleoSpin
®
however, may refer to the Protocol-at-a-glance instead. The Protocol-at-a-glance is
designed to be used only as a supplemental tool for quick referencing while performing
the purification procedure.
All technical literature is available on the internet at www.mn-net.com. Please visit
the MACHEREY-NAGEL website to verify that you are using the latest revision of this
user manual.
Please contact Technical Service regarding information about changes of the current
user manual compared to previous revisions.
Plasmid DNA purification
Plasmid EasyPure kit is used for the first time. Experienced users,
MACHEREY-NAGEL – 08/ 2 013, Rev. 01
5
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