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（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex product.
Shodex™ C18U series is reverse phase chromatography column in which octadecyl group is bonded to organic
and inorganic hybrid gel. Compared to conventional silica-based reversed phase columns, it has excellent
separation performance and high durability, so it can be used under a variety of analytical conditions and is
suitable for the separation of a wide variety of compounds.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6654011
C18U 2B
F6654012
C18U 2D
Base Material
: Spherical organic and silica hybrid particles modified with octadecyl group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF (Parker Type)
Shipping Solvent : Acetonitrile
Operation Manual
Shodex
C18U 2B, 2D
Column Size (mm)
I.D.
2.0
2.0
SHOWA DENKO K.K. (https://www.shodex.com/)
Particle Size (μm)
Length
50
1.9
150
1.9
*Includes 8% carbon content of organic and silica hybrid gel
ver.210601E
*Carbon (%)
20
20
1
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Summary of Contents for Shodex C18U 2B
- Page 1 1. Introduction Thank you for purchasing the Shodex product. Shodex™ C18U series is reverse phase chromatography column in which octadecyl group is bonded to organic and inorganic hybrid gel. Compared to conventional silica-based reversed phase columns, it has excellent separation performance and high durability, so it can be used under a variety of analytical conditions and is suitable for the separation of a wide variety of compounds.
- Page 2 (3) Recommended injection volume is less than 10 μL per column. (4) For samples containing polymer compounds such as proteins and polysaccharides and samples with a lot of impurities, pretreatment (clean up) by solid phase extraction or the like in advance. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 3 · When restarting the system after column installation or after holding the eluent flow, start the system at less than half of the recommended flow rate. A rapid increase in pressure can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 4 (a cool and dark space is recommended). Refer to section 7.4 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
- Page 6 10. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.
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