Shodex CLNpak EV-2000 Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column adhesive label, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex CLNpak  series is a GPC clean-up column
packed with a styrene divinylbenzene copolymer. It is suitable for clean-up of residual pesticide components in
foods.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F6090001
CLNpak EV-2000
F6090002
CLNpak EV-G
Base Material
: Spherical porous particles of styrene divinylbenzene copolymer
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Ethyl acetate/Cyclohexane = 3/7
Operation Manual
Shodex
CLNpak
Column Size
I.D. (mm)
20.0
20.0
SHOWA DENKO K.K. (https://www.shodex.com/)
EV-2000
Particle
Size (μm)
Length (mm)
300
16
100
16
ver.211118E
Theoretical
Plate Number
(Per Column)
≥ 4,000
(Guard Column)
1

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Summary of Contents for Shodex CLNpak EV-2000

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex CLNpak  series is a GPC clean-up column packed with a styrene divinylbenzene copolymer. It is suitable for clean-up of residual pesticide components in foods.
  • Page 2 Thus, dilution of such samples with eluent is recommended. The approximate maximum lipid load per injection is less than 500 mg. Note · Use of guard column is recommended to protect the analytical column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 3 (2) Check miscibility/solubility of the desired new solvent and the solvent currently filled in the column. (3) When replacing the current solvent with a miscible solvent, first introduce 1:1 mixture of the current solvent and the new solvent, and then replace it with 100 % of the new solvent. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4 9. Column Inspection Inspection method is described in the Certificate of Analysis (CoA). Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations. (1) Theoretical Plate Number (N) (2) Asymmetry Factor (Fas) SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.

This manual is also suitable for:

Clnpak ev-gF6090001F6090002

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