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Summary of Contents for ThermoFisher Scientific QuantStudio 1 Plus
- Page 1 ™ QuantStudio 1 Plus Real-Time PCR System INSTALLATION, USE, AND MAINTENANCE Publication Number MAN0024786 Revision A.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Thermo Fisher Scientific (Shanghai) Instruments Co., Ltd. | T71-6, No. 211, Qin Qiao road, China (Shanghai) Free Trade Zone, China For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC.
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Page 3: Table Of Contents
Contents About this guide ............8 ■... - Page 4 Contents Manage instrument settings ........... . . 31 Manage the instrument name (Administrator only) .
- Page 5 Contents ■ CHAPTER 5 Calibrate and verify instrument performance ..... 52 Calibration and verification schedule ..........52 Calibration descriptions .
- Page 6 Contents ■ APPENDIX A Install and connect the instrument to a network ....80 Workflow: Install and connect to a network ........80 Before you begin .
- Page 7 Contents Safety and electromagnetic compatibility (EMC) standards ......102 Safety compliance ............102 EMC .
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Page 8: About This Guide
About this guide ™ This guide provides information about installing, using, and maintaining the QuantStudio 1 Plus Real- Time PCR System. For information and instructions on performing experiments on this system, see the ™ QuantStudio Design and Analysis SC Software v1.0 User Guide (Pub. No. MAN0024784). QuantStudio ™... -
Page 9: Chapter 1 Product Information
Product information ■ Instrument hardware description ............9 ■... - Page 10 Chapter 1 Product information Instrument hardware description An optional barcode scanner and optional wireless adapter can be ordered separately. Instrument filters and supported dyes System dyes The instrument uses a coupled four-color filter set that supports the dyes shown in the following table and figure.
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Page 11: Parts Of The Instrument
Chapter 1 Product information Instrument hardware description To use a custom dye on the instrument, review the following requirements: • Calibrate the instrument for the custom dye (see “Calibrate custom dyes” on page 65). • Ensure that the custom dye excites and emits within the wavelength range of the filters (see Table 1 on page 10). -
Page 12: Parts Of The Home Screen
Chapter 1 Product information Instrument hardware description Parts of the home screen Instrument name Settings button Help icon Buttons for accessing experiment template files (EDT) Drawer status Connectivity icons Status dial Sign In (or My Profile) button Current user name and instrument block type Table 2 Parts of the home screen Part of the home Function... - Page 13 Chapter 1 Product information Instrument hardware description Table 2 Parts of the home screen (continued) Part of the home Function More information screen • — The drawer is locked. The drawer locks Drawer status — during experiment runs, calibration runs, and self verification test runs.
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Page 14: Software Description
Chapter 1 Product information Software description Table 2 Parts of the home screen (continued) Part of the home Function More information screen • — The instrument is connected to a wired Connectivity — network. icons • — The instrument is wirelessly connected. •... -
Page 15: Folders, Templates, Experiments, And Projects
Chapter 1 Product information Software description (continued) ™ Thermo Fisher Software feature Instrument Desktop Connect Change experiment settings in template (EDT file) — — ✓ loaded on the instrument Settings that can be changed in a locked template (no password required): •... - Page 16 Chapter 1 Product information Software description (continued) Term Definition Supported in Experiment Template Default settings for an instrument run, can be modified before Instrument File (EDT) instrument run. Desktop Two types: ™ Thermo Fisher • Factory-provided, accessed from Open Template Connect (instrument) or Create Experiment (desktop or Thermo ™...
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Page 17: Third-Party Software
Chapter 1 Product information Supported options for instrument and computer connections (continued) Template and ™ experiment Instrument Desktop Thermo Fisher Connect components Results Not applicable Review plots Export Not applicable Export results Third-party software ™ Before installing third-party software on the computer running QuantStudio Design and Analysis SC Software v1.0, confirm that the third-party software will not do the following: •... - Page 18 Chapter 1 Product information Supported options for instrument and computer connections ™ Note: For detailed information about networking your instrument, see the QuantStudio 1 Plus Real- Time PCR System IT Checklist (Pub. No. MAN0017226). Direct configuration option • A computer provided by Thermo Fisher Scientific with the QuantStudio ™...
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Page 19: Experiment Types
Chapter 1 Product information Experiment types Experiment types Purpose Description Standard curve experiment Determines absolute 1. The software measures amplification of the target in a standard dilution series and in target quantity in test samples. samples. 2. The software generates a standard curve using data from the standard dilution series. 3. - Page 20 Chapter 1 Product information Experiment types Purpose Description Genotyping experiment Detects single ™ Genotyping experiments use preformulated TaqMan SNP Genotyping Assays that include nucleotide the following components: polymorphism (SNP) • Two sequence-specific primers for amplification of sequences containing the SNP of variants of a interest target nucleic acid...
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Page 21: Chapter 2 General Procedures To Operate The Instrument
General procedures to operate the instrument ■ Precautions for use ..............21 ■... -
Page 22: Power On The Instrument
Chapter 2 General procedures to operate the instrument Power on the instrument Power on the instrument 1. Touch anywhere on the touchscreen to determine if the instrument is in sleep mode. If the home screen is displayed, the instrument is already powered on. 2. -
Page 23: Sign Out
Chapter 2 General procedures to operate the instrument Sign out Sign out 1. In the home screen, touch My Profile. 2. Touch Sign Out. ™ Load and unload a plate in the QuantStudio 1 Plus Real- Time PCR Instrument CAUTION! Use flat caps for 0.2-mL tubes. Rounded caps can damage the heated cover. 1. -
Page 24: Transfer, View, Or Manage Files And Results
Chapter 2 General procedures to operate the instrument Transfer, view, or manage files and results CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the plate temperature can reach 100°C. Allow it to cool to room temperature before handling. Transfer, view, or manage files and results Transfer EDS files from the instrument home screen 1. -
Page 25: Manage Templates (Edt Files)
Chapter 2 General procedures to operate the instrument Transfer, view, or manage files and results Manage templates (EDT files) This feature applies to templates (EDT files) on a USB or in My Instrument. To manage run results files (EDS files), see “Transfer EDS files from the instrument home screen” on page 1. -
Page 26: Chapter 3 Configure The Instrument And Manage Instrument Profiles
Configure the instrument and manage instrument profiles ■ Initial start‑up ............... . 26 ■... -
Page 27: Installation And Instrument Verification
Chapter 3 Configure the instrument and manage instrument profiles Installation and instrument verification 5. Create an administrator profile (see “Create an administrator instrument profile during initial start- up” on page 27). 6. Select one of the following options. • Link the instrument profile to Thermo Fisher ™... -
Page 28: Create A New Instrument Profile
Chapter 3 Configure the instrument and manage instrument profiles Create a new instrument profile 1. Touch Name, enter a username, then touch Done. 2. Touch PIN, enter a four-digit numerical password, then touch Enter. Note: Touch the Show PIN checkbox to switch PIN display on or off. 3. -
Page 29: Overview Of Settings
Chapter 3 Configure the instrument and manage instrument profiles Overview of Settings Overview of Settings Touch Settings in the home screen to configure settings as needed. Access to many settings are restricted to administrator instrument profiles. Touch for step-by-step instructions on configuring settings. Options Description Instrument Settings... - Page 30 Chapter 3 Configure the instrument and manage instrument profiles Overview of Settings Options Description OEM Connection Only Required for API access to the instrument. When enabled, the software (desktop and Thermo Fisher ™ Connect) cannot connect to the instrument. (Administrator only) API access to the instrument is exclusive to authorized OEM partners.
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Page 31: Manage Instrument Settings
Chapter 3 Configure the instrument and manage instrument profiles Manage instrument settings Options Description • Perform calibrations Calibrations – ROI and Uniformity – Dye – Custom (including Background calibration) • View calibration history and set calibration reminders in History and Reminders RNase P Verification Perform instrument performance verification using an RNase P plate. -
Page 32: Manage The Instrument Name (Administrator Only)
Chapter 3 Configure the instrument and manage instrument profiles Manage instrument settings “OEM connection only (Administrator only)” on page 33 ™ “Select a region for Thermo Fisher Connect (Administrator only)” on page 33 “Set the date and time (Administrator only)” on page 33 “Configure the network”... -
Page 33: Oem Connection Only (Administrator Only)
Chapter 3 Configure the instrument and manage instrument profiles Manage instrument settings OEM connection only (Administrator only) This setting is required for API access to the instrument. When enabled, the desktop software cannot connect to the instrument. Do not access unless instructed to do so by a service representative. Select a region for Thermo Fisher ™... - Page 34 Chapter 3 Configure the instrument and manage instrument profiles Manage instrument settings Set up a wired connection 1. In the home screen, touch Settings4Instrument Settings4Network Connection4 Wired. 2. In the Network Configuration screen, touch a radio button to connect to a network either by DHCP or by a Static IP address.
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Page 35: Restore Factory Defaults
Chapter 3 Configure the instrument and manage instrument profiles Learn about the instrument Restore factory defaults This procedure can only be performed by an Administrator. IMPORTANT! Back up the instrument before restoring factory defaults (see “Backup or restore the instrument” on page 73). -
Page 36: View And Export The License Agreement
Chapter 3 Configure the instrument and manage instrument profiles Manage home screen notifications • Instrument Server • User Interface View and export the License Agreement 1. In the home screen, touch Settings4About Instrument4License Agreement to view the license agreement. 2. Touch Export to export the license agreement. 3. -
Page 37: Enable Remote Instrument Monitoring (Administrator Only)
Chapter 3 Configure the instrument and manage instrument profiles Manage maintenance, service, and calibrations Enable remote instrument monitoring (Administrator only) 1. In the home screen, touch Settings4Maintenance and Service4Monitoring. 2. Configure the instrument monitoring settings. Option Description Remote Monitoring Slide the control On to enable the Remote Monitoring Service. Service Enabling the Remote Monitoring Service allows the instrument to automatically notify Thermo Fisher Scientific support teams in real time of... -
Page 38: Manage Users
Chapter 3 Configure the instrument and manage instrument profiles Manage Users Manage Users In the home screen, touch Settings4Manage Users to access the following functions. “Require instrument profile sign-in (Administrator only)” on page “Manage the Sign Out Timer (Administrator only)” on page “Manage all instrument profiles (Administrator only)”... -
Page 39: Link An Instrument Profile To Thermo Fisher
Chapter 3 Configure the instrument and manage instrument profiles Link an instrument profile to Thermo Fisher ™ Connect Edit an instrument profile 1. In the home screen, touch My Profile. Note: Administrators can also navigate to this screen by touching Settings4Manage Users4Manage Profiles. -
Page 40: Link An Existing Instrument Profile To Thermo Fisher
Chapter 3 Configure the instrument and manage instrument profiles Link an instrument profile to Thermo Fisher ™ Connect Link an existing instrument profile to Thermo Fisher ™ Connect 1. Sign in to your instrument profile. See “Sign in” on page 2. -
Page 41: Workflow
Create and run experiments on the instrument ■ Workflow ................41 ■... -
Page 42: Chapter 4 Create And Run Experiments On The Instrument
Chapter 4 Create and run experiments on the instrument Run an experiment Run an experiment Create and run an experiment from a template 1. In the home screen, touch Open Template. 2. (Optional) Touch a category in the left column. 3. -
Page 43: Repeat The Last Instrument Run
Chapter 4 Create and run experiments on the instrument Run an experiment 6. (Optional) Edit the run method (see “Edit the run method” on page 45). • Add, remove, or edit a step, stage, melt curve, or data collection point. •... -
Page 44: Edit An Experiment Before Starting A Run
Chapter 4 Create and run experiments on the instrument Edit an experiment before starting a run Edit an experiment before starting a run Enter or edit template properties Access a template (EDT file). For more information, see the following sections. •... -
Page 45: Edit The Run Method
Chapter 4 Create and run experiments on the instrument Edit an experiment before starting a run Scan a barcode using the optional barcode scanner The instrument is compatible with an optional Handheld Barcode Scanner (Cat. No. 4488442, purchased separately). The barcode scanner reads Code 128 (alphanumeric), which supports 128 ASCII character barcodes. - Page 46 Chapter 4 Create and run experiments on the instrument Edit an experiment before starting a run Method elements Method tab Number of cycles for the stage Heated cover temperature Temperature for the step Reaction volume Hold time for the step Stage of thermal protocol Data collection point Step within a stage...
- Page 47 Chapter 4 Create and run experiments on the instrument Edit an experiment before starting a run (continued) Option Procedure 1. Touch Add/Remove stages4Add stages. 2. Touch on the left or right border of a stage to add a stage before or after the stage, respectively.
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Page 48: Define, Assign, And View Well Details
Chapter 4 Create and run experiments on the instrument Edit an experiment before starting a run Configure ramp rates and pause settings • In the Method screen, touch Edit4Manage Steps4Advanced Options. Option Procedure 1. Touch Ramp Rates. 2. Touch the ramp rates fields. Edit Ramp Rates 3. -
Page 49: View, Pause, Or Stop A Run
Chapter 4 Create and run experiments on the instrument View, pause, or stop a run (continued) Option Procedure View target information 1. Touch 2. Touch Manage or touch an individual well. 3. Touch the Targets subtab. 4. Touch Details to view target information, then touch Done. 3. -
Page 50: Pause Or Stop An Instrument Run
Chapter 4 Create and run experiments on the instrument Transfer EDS files from the instrument home screen Pause or stop an instrument run 1. In the home screen, during an instrument run, touch or swipe left once to view real‑time run method information. - Page 51 Chapter 4 Create and run experiments on the instrument Transfer EDS files from the instrument home screen Note: Touch Settings4Run History to transfer EDS files at any time. QuantStudio ™ 1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide...
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Page 52: Chapter 5 Calibrate And Verify Instrument Performance
Calibrate and verify instrument performance ■ Calibration and verification schedule ........... . 52 ■... -
Page 53: Calibration Descriptions
Chapter 5 Calibrate and verify instrument performance Calibration descriptions (continued) Calibration Recommended frequency • Every two years (recommended) Background • Background calibration can also be performed, as needed: – To check for contamination (depends on usage and laboratory conditions). – To obtain the most accurate data for the removal of background fluorescence. -
Page 54: View The Calibration Status And Set Reminders
Chapter 5 Calibrate and verify instrument performance View the calibration status and set reminders (continued) Calibration description and purpose Pass Criteria • The software extracts a spectral profile for each dye standard, then Dye spectra peak within the same produces a set of spectral profiles plotted as fluorescence vs filter. filter as their group. -
Page 55: Perform Roi/Uniformity, Background, And Dye Calibrations
Chapter 5 Calibrate and verify instrument performance Perform ROI/uniformity, background, and dye calibrations 3. In the Summary tab: • Click Calibrations to view the status of each calibration type. • (Optional) Click Calibration Reminders to set the calibration reminder time table and enter the notification email address(es). -
Page 56: Prepare A Calibration Plate
Chapter 5 Calibrate and verify instrument performance Perform ROI/uniformity, background, and dye calibrations Prepare a calibration plate Materials required for calibration plate preparation • Plate(s) for the calibration that you are performing: – ROI plate (the same plate is used for ROI and uniformity calibrations) –... -
Page 57: Perform Calibrations
Chapter 5 Calibrate and verify instrument performance Perform ROI/uniformity, background, and dye calibrations IMPORTANT! Keep the bottom of the plate clean. Fluids and other contaminants on the bottom of the plate can contaminate the sample block and cause an abnormally high background signal. Perform calibrations 1. -
Page 58: View Calibration Images And Transfer Results To Usb
Chapter 5 Calibrate and verify instrument performance Perform ROI/uniformity, background, and dye calibrations View calibration images and transfer results to USB The instrument performs the ROI, Uniformity, and Background calibrations in sequence. You can view the calibration images after the Background calibration is complete. 1. -
Page 59: Troubleshoot Calibration Failure
Chapter 5 Calibrate and verify instrument performance Perform ROI/uniformity, background, and dye calibrations Troubleshoot calibration failure Observation Possible cause Recommended action Calibration failed The plate was improperly Ensure the following: prepared. • The correct plate was used for the calibration performed. •... -
Page 60: Create A Background Plate (Optional)
Chapter 5 Calibrate and verify instrument performance Perform instrument verification using RNase P plates 3. Determine the location of the failed wells again as in step 1. Position of failed wells Action Identical The sample block is contaminated. Decontaminate the sample block (see page 74). -
Page 61: Instrument Verification Description
Chapter 5 Calibrate and verify instrument performance Perform instrument verification using RNase P plates The instrument requires valid ROI/uniformity, background, and dye calibrations to perform instrument verification. Instrument verification description Purpose Description Pass criteria Confirms the Quantifies the number of copies of The instrument passes performance specifications performance of the the human RNase P gene in samples... -
Page 62: Performance Specifications Pass Criteria
Chapter 5 Calibrate and verify instrument performance Perform instrument verification using RNase P plates Performance specifications pass criteria After the run, the software calculates average copy number values and standard deviation values. The instrument passes performance specifications if the following inequality is true and the instrument successfully distinguishes between unknown populations A and B with a statistical confidence level of 99.7%. -
Page 63: Perform Rnase P Verification
Chapter 5 Calibrate and verify instrument performance Perform instrument verification using RNase P plates 5. Confirm that the liquid in each well is at the bottom of the well and free of bubbles. If it is not, centrifuge the plate again. IMPORTANT! Keep the bottom of the plate clean. -
Page 64: Troubleshoot Verification Failure
Chapter 5 Calibrate and verify instrument performance Perform instrument verification using RNase P plates Troubleshoot verification failure Observation Possible cause Recommended action Verification failed The plate was improperly Ensure the following: prepared. • The correct plate was used for the verification performed. -
Page 65: Calibrate Custom Dyes
Chapter 5 Calibrate and verify instrument performance Calibrate custom dyes Calibrate custom dyes Custom dyes overview The instrument can run assays designed with custom dyes. Custom dyes include: • Dyes that are not manufactured by Thermo Fisher Scientific. • Dyes or formulations of dyes that are not system dyes for the instrument. •... - Page 66 Chapter 5 Calibrate and verify instrument performance Calibrate custom dyes Prepare a custom dye dilution plate IMPORTANT! Wear powder-free gloves throughout the procedure. 1. Prepare a 2- or 3-fold dilution series of the custom dye. 2. Dispense aliquots of each dilution into the center of a reaction plate, then seal the plate. A full plate is not needed.
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Page 67: Calibrate The Custom Dye
Chapter 5 Calibrate and verify instrument performance Calibrate custom dyes • Create a new experiment on the instrument touchscreen. a. In the home screen, touch Open Template4Genotyping4Genotyping Post. b. (Optional) In the Properties tab, edit the experiment properties. c. In the Method tab, set the hold temperature to 60°C with a 2 minute hold and enter the appropriate reaction volume. - Page 68 Chapter 5 Calibrate and verify instrument performance Calibrate custom dyes 4. Vortex the plate for 5 seconds, then centrifuge at 750–1,000 × g for 2 minutes. 5. Confirm that the liquid in each well is at the bottom of the well and free of bubbles. If it is not, centrifuge the plate again.
- Page 69 Chapter 5 Calibrate and verify instrument performance Calibrate custom dyes Perform a custom dye calibration 1. Load the plate into the instrument. 2. In the instrument home screen, touch Settings4Maintenance and Service4Calibrations4Custom4Custom Dye. 3. Touch the custom dye to calibrate. 4.
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Page 70: Calibrate For A Custom Melt Curve Experiment
Chapter 5 Calibrate and verify instrument performance Calibrate for a custom melt curve experiment 10. Select an action depending on whether the custom dye calibration passed or failed. Calibration status Action Passed • Touch Accept Results or Reject Results. Note: Accepting the results saves the calibration data to the instrument and overwrites existing data. - Page 71 Chapter 5 Calibrate and verify instrument performance Calibrate for a custom melt curve experiment 4. Select or add a dye, then select a filter set appropriate for your dye's wavelength (see filter- wavelength table below). Note: Refer to your reagent kit documentation for dye name and wavelength information. Filter wavelength Peak filter Excitation...
- Page 72 Chapter 5 Calibrate and verify instrument performance Calibrate for a custom melt curve experiment 9. Select an action depending on whether the custom dye calibration passed or failed. Calibration status Action Passed • Touch Accept Results or Reject Results. Note: Accepting the results saves the calibration data to the instrument and overwrites existing data.
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Page 73: Chapter 6 Maintain The Instrument
Maintain the instrument ■ Backup or restore the instrument ............73 ■... -
Page 74: Decontaminate The Sample Block
Chapter 6 Maintain the instrument Decontaminate the sample block Decontaminate the sample block Perform this procedure to eliminate fluorescent contaminants from the instrument sample block. Contamination is generally evident in failed background calibrations where one or more wells consistently exhibit abnormally high signals. CAUTION! PHYSICAL INJURY HAZARD. - Page 75 Chapter 6 Maintain the instrument Decontaminate the sample block b. Pull the instrument drawer forward to expose the sample block. 3. Rinse the contaminated wells with deionized water (see “Detailed procedures for cleaning the sample block” on page 76). 4. Close the drawer and test the sample block for contamination. a.
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Page 76: Detailed Procedures For Cleaning The Sample Block
Chapter 6 Maintain the instrument Decontaminate the sample block Detailed procedures for cleaning the sample block IMPORTANT! Use these cleaning procedures only in conjunction with the complete decontamination procedure (see “Decontaminate the sample block” on page 74). • Rinse the sample block with deionized water. a. -
Page 77: Replace The Instrument Fuses
Chapter 6 Maintain the instrument Replace the instrument fuses Replace the instrument fuses CAUTION! FIRE HAZARD. For continued protection against the risk of fire, replace fuses only with listed and certified fuses of the same type and rating as those currently in the instrument. Materials required •... -
Page 78: Prepare The Instrument To Store, Move, Or Ship
Chapter 6 Maintain the instrument Prepare the instrument to store, move, or ship Note: Fuse failure can result from fluctuations in the supplied power to the system. To prevent further failures, consider installing an electrical protective device, such as a UPS or a surge protector. If issues with the fuse persist, contact Support. -
Page 79: Return The Instrument For Service
Chapter 6 Maintain the instrument Prepare the instrument to store, move, or ship Return the instrument for service The service process requires 2 to 3 weeks. Before returning the instrument for service, perform the following tasks. 1. Back up the instrument (see “Backup or restore the instrument” on page 73). -
Page 80: Workflow: Install And Connect To A Network
Install and connect the instrument to a network ■ Workflow: Install and connect to a network ..........80 ■... -
Page 81: Appendix A Install And Connect The Instrument To A Network
Appendix A Install and connect the instrument to a network Before you begin Before you begin This section provides instructions for customer installation of the computer and the instrument. For installation by a field service engineer (FSE), contact Support to order a service call. Before starting the installation, complete the following tasks: •... -
Page 82: Unpack And Install The Instrument
Appendix A Install and connect the instrument to a network Unpack and install the instrument Figure 5 Instrument‑to‑computer connections (barcode scanner connected to the computer) Minitower configuration Detachable power supply cord compatible with local power supply receptacle. Connection between the computer and the instrument. Connection between the computer and the monitor, keyboard, and mouse. -
Page 83: Power On And Follow The Startup Wizard
Appendix A Install and connect the instrument to a network Power on and follow the startup wizard 5. Connect your instrument as required by your network configuration (see “Supported options for instrument and computer connections” on page 17 and “Instrument and computer connections” on page 81). -
Page 84: Connect The Instrument And The Computer Directly Or To A Lan
Appendix A Install and connect the instrument to a network Connect the instrument and the computer directly or to a LAN Connect the instrument and the computer directly or to a This section describes direct wired connection of the computer provided by Thermo Fisher Scientific to the instrument or to a LAN. -
Page 85: Networking
Appendix A Install and connect the instrument to a network Networking Networking IMPORTANT! This section provides general networking information. It does not provide adequate detail to integrate the instrument into all possible network architectures. Because a network may contain advanced features (such as a firewall or network domains), we recommend that you consult a network administrator before connecting the instrument to your laboratory network. -
Page 86: Control And Monitor Networked Instruments
Appendix A Install and connect the instrument to a network Networking (continued) Networked configuration option • Instrument‑to‑LAN connection (select an option): – Wired connection to the network using an Ethernet cable – or – – Wireless connection to the network using the instrument Wi‑Fi module ™... -
Page 87: Ethernet Port Overview
Appendix A Install and connect the instrument to a network Networking Ethernet port overview The Ethernet port of the instrument supports: • Static IP network service with subnet mask, primary and secondary data network service (DNS), and default gateway settings, or dynamic host configuration protocol (DHCP) network service. •... -
Page 88: Appendix B Troubleshooting
Troubleshooting Observation Possible cause Recommended action Inconsistent communication The instrument is configured Ensure only one connectivity option is plugged between instrument and for both wired and wireless into the instrument (either an Ethernet cable or a computer or instrument and network connection. - Page 89 Appendix B Troubleshooting Networking Observation Possible cause Recommended action Forgot PIN for instrument profile Administrator forgot instrument Have another administrator reset the PIN for the (continued) profile PIN. forgotten-PIN profile (see “Manage all instrument profiles (Administrator only)” on page 39). If there is not another administrator profile on the instrument, you must restore factory defaults (see “Restore factory defaults”...
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Page 90: Appendix C Instrument Specification And Layout
Instrument specification and layout ■ Configured system dimensions ............91 ■... -
Page 91: Configured System Dimensions
Appendix C Instrument specification and layout Configured system dimensions Configured system dimensions Allow space for the configured instrument. A typical setup with a co-located minitower computer is shown below. Note: Dimensions are rounded to the nearest whole or half unit. 15 cm (6 in.) 27 cm (10.5 in.) 30 cm (12 in.) -
Page 92: Instrument Clearances
Appendix C Instrument specification and layout Electrical requirements Instrument clearances During instrument installation and maintenance, it is necessary to access the back of the instrument. If the back of the instrument faces a wall, ensure that there is sufficient clearance on the bench to rotate the instrument for access. -
Page 93: Environmental Requirements
Appendix C Instrument specification and layout Environmental requirements Environmental requirements Table 3 Environmental requirements Condition Acceptable range Installation site Indoor use only Electromagnetic interference Do not use this device in close proximity to sources of strong electromagnetic radiation (for example, unshielded intentional RF sources). Strong electromagnetic radiation may interfere with the proper operation of the device. - Page 94 Appendix C Instrument specification and layout Network requirements The instrument can be configured for either wired or wireless networking, not both. If a Thermo Fisher Scientific service representative is to install the instrument: • If the instrument will be connected to a LAN, an active, tested network jack must be in place before the scheduled installation date.
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Page 95: Appendix D Parts And Materials
Parts and materials ■ Kits, consumables, accessories, and reagents ......... . . 95 ■... -
Page 96: Accessories
Appendix D Parts and materials Accessories (continued) Instrument verification or calibration plate Cat. No. ™ QuantStudio 3/5 Spectral Calibration Plate 2, 96‑Well 0.2‑mL A26332 ™ ™ ™ (ABY , JUN , and MUSTANG PURPLE dyes) ™ QuantStudio 3/5 Spectral Calibration Plate 3, 96‑Well 0.2‑mL A26332 (TAMRA ™... - Page 97 Appendix D Parts and materials General-use materials and consumables (continued) Material/Consumable Source Pipettors, 100‑µL and 200‑µL (with pipette tips) Powder‑free gloves Safety glasses Screwdriver, flathead Tissue, lint‑free Deionized water QuantStudio ™ 1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide...
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Page 98: Appendix E Safety
Safety ■ Symbols on this instrument ............98 ■... -
Page 99: Conformity Symbols
Appendix E Safety Symbols on this instrument (continued) Symbol English Moving parts Caution, hot surface Protective conductor terminal (main ground) Do not dispose of this product in unsorted municipal waste CAUTION! To minimize negative environmental impact from disposal of electronic waste, do not dispose of electronic waste in unsorted municipal waste. -
Page 100: Safety Alerts On This Instrument
Appendix E Safety Safety alerts on this instrument Safety alerts on this instrument Additional text may be used with one of the symbols described above when more specific information is needed to avoid exposure to a hazard. See the following table for safety alerts found on the instrument. -
Page 101: Safety Information For Instruments Not Manufactured By Thermo Fisher Scientific
Appendix E Safety Safety information for instruments not manufactured by Thermo Fisher Scientific Safety information for instruments not manufactured by Thermo Fisher Scientific Some of the accessories provided as part of the instrument system are not designed or built by Thermo Fisher Scientific. -
Page 102: Cleaning And Decontamination
Appendix E Safety Safety and electromagnetic compatibility (EMC) standards Cleaning and decontamination CAUTION! Cleaning and Decontamination. Use only the cleaning and decontamination methods specified in the manufacturer's user documentation. It is the responsibility of the operator (or other responsible person) to ensure the following requirements are met: ·... -
Page 103: Emc
Appendix E Safety Safety and electromagnetic compatibility (EMC) standards Reference Description Directive 2014/30/EU European Union “EMC Directive” EN 61326-1/ IEC 61326-1 Electrical Equipment for Measurement, Control and Laboratory Use – EMC Requirements – Part 1: General Requirements AS/NZS CISPR 11 Limits and Methods of Measurement of Electromagnetic Disturbance Characteristics of Industrial, Scientific, and Medical (ISM) Radiofrequency Equipment... -
Page 104: Chemical Safety
Appendix E Safety Chemical safety Chemical safety WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below. Consult the relevant SDS for specific precautions and instructions: ·... -
Page 105: Biological Hazard Safety
Appendix E Safety Biological hazard safety Biological hazard safety WARNING! Potential Biohazard. Depending on the samples used on this instrument, the surface may be considered a biohazard. Use appropriate decontamination methods when working with biohazards. WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. -
Page 106: Appendix F Documentation And Support
Documentation and support Related documentation Document Publication number QuantStudio ™ Design and Analysis SC Software v1.0 User Guide MAN0024784 ™ QuantStudio 1 Plus Real-Time PCR System Site Preparation Guide MAN0024787 Customer and technical support Visit thermofisher.com/support for the latest service and support information. •... -
Page 107: Index
Index custom dye add to the instrument 68 accessories 96 calibration plate, create 67 administrator instrument profile 27 calibration, perform 69 description 10 dilution guidelines 65 optimal concentration 67 background calibration wavelength requirements 65 create plate 60 custom dye calibration, workflow 65 description 53 custom dye dilution plate, prepare 66 plate, prepare 56... - Page 108 Index fluorescence high melt curve experiments 19 fuses, replace 77 melt curves, add to method 46 method add melt curves 46 add stages 46 genotyping experiments 19 add steps 46 guest profile data collection points 46 disable 38 edit 45 limits 27 parts of 46 pause 48...
- Page 109 Index RNase P verification, plate, prepare 62 system dyes 10 ROI/uniformity calibration description 53 plate, prepare 56 ROI/Uniformity calibration template properties. See properties terms and conditions 106 run 57 thermal protocol. See method when to perform 52 run, pause or stop 50 third-party software 17 run data, transfer 24, 50 transfer files 24...
- Page 110 QS 1 Plus_UG_RUO_MAN0024786-v6-GUID-D0087F29-145E-49AF-A401-9309CA54F0CA-2021/04/08 03:12:29 en 03:13:35.363+01:00 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 8 April 2021...
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