Molecular Devices SpectraMax Mini User Manual page 34

SpectraMax Mini User Guide
Kinetic analysis has many advantages to determine the relative activity of an enzyme in
different types of plate assays, including ELISAs and the purification and characterization of
enzymes and enzyme conjugates. Kinetic analysis is capable of providing improved dynamic
range, precision, and sensitivity relative to endpoint analysis.
Spectrum
For the absorbance read mode, the Spectrum read type measures optical density (OD) or
%Transmittance across a specified wavelength range, with allowed values from 200 nm to 1000
nm.
Well Scan
The Well Scan read type takes reads at more than one location on an evenly spaced, user
defined grid or other pattern inside of each well at single or multiple wavelengths. The fill
pattern is either round or square to match the well shape. The number of points that are
available depend on the well size of the plate you select.
The Read Order you select in the More Settings category determines the available options.
For the Row and Well read order, select:
For a Column read order, select:
Drag the Density slider and the Point Spacing slider to define the well scan specifications. The
values for Density and Point Spacing are dependent on each other and on the plate you select.
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