Centering The Darkfield Diaphragm Of The Condenser; Centering The Annular Phase Diaphragm Of The Condenser - Zeiss Axioscope 5 Instruction Manual

ZEISS

10.14.2 Centering the Darkfield Diaphragm of the Condenser

Parts and Tools

Prerequisite
ü
ü
Procedure 1. Set the modulator disks to position D (or DF = darkfield).
2. Remove one eyepiece from the binocular tube or replace it with the auxiliary microscope.
3. Observe the exit pupil of the objective.
4. Turn the two centering screws, until the exit
5. Insert the eyepiece.

10.14.3 Centering the Annular Phase Diaphragm of the Condenser

Parts and Tools

Prerequisite ü
ü
Procedure 1. Set the modulator disks to position
2. Remove one eyepiece from the binocular tube or replace it with the auxiliary microscope.
3. Observe the exit pupil of the objective.
4. Check the centering and the overlap of the
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001
10 Accessories and System Expansions | 10.14 Condenser, achromatic-aplanatic 0.9 BF DF PhC DIC
2 x Hex key, 1.5 mm
A suitable condenser with modulator disk is installed.
The illumination is adjusted for transmitted light brightfield microscopy.
pupil of the objective appears homogeneously
dark.
2 x Hex key, 1.5 mm
A suitable condenser with modulator disk is installed.
The illumination is adjusted for transmitted light brightfield microscopy.
lighter annular phase diaphragm (in the con-
denser) with the darker phase ring (in the ob-
jective).
Both rings must be centered and overlapping
B
.
Ph (phase contrast).
A B
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