Setting The Darkfield Contrast With A Dry Darkfield Condenser - Zeiss Axioscope 5 Instruction Manual

5 Operation | 5.4 Setting Up for Transmitted Light Techniques

5.4.3 Setting the Darkfield Contrast with a Dry Darkfield Condenser

Prerequisite ü
ü
ü
Procedure 1. Move the condenser up until the end stop .
2. Place the sample on the stage.
3. Adjust the illumination intensity sufficiently bright.
4. Swivel in an objective with small magnification (e.g. 5x or 10x)
5. Focus the sample.
6. Place a sample so that its details are evenly visible in the field of view.
7. Close the luminous-field diaphragm until the end stop.
8. Lower condenser until the edge of the field diaphragm appears sharp (luminous-field di-
9. Center the field diaphragm image with both centering screws on the condenser carrier.
10. Swivel in the desired objective.
11. If necessary, focus the sample.
12. Focus the luminous-field diaphragm with the knurled knob for vertical adjustment.
13. Open the luminous-field diaphragm enough to make the edge of the diaphragm disappear
94
The microscope is operational.
The dry darkfield condenser is installed [
Low-power system, polarizer or Lambda plate are swivelled out of the beam path.
à
The image of the field diaphragm is easier to identify.
aphragm focus level).
à An increasing or decreasing light ring is visible, when moving the focus upwards or
downwards from the field diaphragm focus level (so called circular "breathing" of the
field diaphragm depiction).
from the field of view.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001
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