Table of Contents
Advertisement
6.1 DEVISE REQUIREMENTS AND TEST CONDITIONS
An analytical balance must be used. The balance selection
depends upon selected model of the pipette and sensitivity of
balance reading.
Test liquid: Water, distilled or de-ionized, grade 3 water
conforming ISO3696 Calibration should be carried out in a
draft-free room at a constant (±0.5°C) temperature of water,
pipette and air between 15°C to 30°C.
The relative humidity must be above 50% especially with
volumes under 50 µl, the air humidity should be as high as
possible to reduce the effect of evaporation loss.
Special accessories for analytical balance, such as the
evaporation trap are recommended for the calibration of
volumes under 50 µl.
6.2 CALIBRATION ADJUSTMENT
1.
Calibration adjustment is done with the calibration tool
provided along with pipette.
2.
Rotate the volume locking lever to the "lock" position so
the volume setting mechanism is locked and able to turn
the calibration screw.
3. Remove volume adjustment knob by pulling it upwards.
4. Place the calibration tool into the calibration grooves.
5.
Rotate the calibration tool counter clockwise to increase
and clockwise to decrease the volume.
6.
After adjustment, check the calibration according to the
instruction in point number 6.3
7.
Once within permissible error range, remove the
calibration tool from pipette and place volume
adjustment knob in its original position.
Depending upon use, we recommend checking of
calibration every six months. However this can be adjusted to
individual requirements.
6.3 PROCEDURE TO CHECK CALIBRATION
The pipette is checked at maximum volume, at 50 % of
maximum volume and at minimum or 10% of maximum
volume, whichever is higher.
•
A new tip is first pre-wetted 3-5 times and a series of ten
pipetting is done at each volume.
•
Use of forward pipetting technique is recommended.
- 10 -
Advertisement
Table of Contents
