TABLE OF CONTENTS Sr. No. Description Page # Introduction Operating instructions for using this manual Danger symbols and danger levels Intended use Package contents Product description Variable volume pipette range Fixed volume pipette range Multi channel pipette range Setting the volume Volume indicator display example Materials Pipette operation...
INTRODUCTION You are now the proud owner of one of the most precise and lowest plunging force required mechanical pipette. This manual will guide you on how to take care of your pipette and benefit the most from its advanced features as follows: •...
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SINGLE CHANNEL VARIABLE VOLUME Volume adjustment knob Volume lock Tip ejector pusher Volume range Coupler Tip ejector Tip cone - 2 -...
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MULTICHANNEL VARIABLE VOLUME Volume adjustment knob Volume lock Tip ejector pusher Volume range Coupler Manifold Tip cone - 3 -...
• After pipetting acids or other corrosive liquids that emit vapors, remove the tip-cone and rinse the piston, O-ring and seal with distilled water. • Do not pipette liquids having temperatures above 70°C. (Not recommendable) • Make sure that liquids never enter the tip-cone. To prevent this: Avoid laying the pipette horizontally when tip is filled with sample.
Setting the volume beyond the allowable volume range is not permitted. Using excessive force to turn the volume adjustment knob outside the permitted range will jam the mechanism, damage the pipette and void the warranty. 5.3 LOADING TIPS Using correct pipette tip according to the pipette volume range is recommended.
5.6 REVERSE PIPETTING The reverse pipetting technique is suitable for dispensing liquids that have high viscosity or a tendency to foam easily. This technique is also recommended for dispensing very small volumes. Press the operating knob until the second stop. Dip the tip under the surface of the liquid in the reservoir and slowly release the operating knob.
6.1 DEVISE REQUIREMENTS AND TEST CONDITIONS An analytical balance must be used. The balance selection depends upon selected model of the pipette and sensitivity of balance reading. Test liquid: Water, distilled or de-ionized, grade 3 water conforming ISO3696 Calibration should be carried out in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C to 30°C.
• Calculate the inaccuracy and imprecision for all three volumes as per EN ISO 8655-6 standards on the basis of the following calculation 6.3.1 CONVERSION OF WEIGHT READINGS TO VOLUME Mean Volume Mean Weight Balance Reading number of reading Conversion factor [example Z=1.0040 µl/mg at 25°c and 1013 hPa] 6.3.2 CALCULATION FOR IN-ACCURACY (SYSTEMATIC ERROR) Mean Value...
7.2.1 DISASSEMBLING THE SINGLE-CHANNEL PIPETTE UP TO 1ml 1. Unscrew the coupler and remove tip cone. Piston Spring 2. Remove the lower part and pull out the piston and other parts from tip cone. Retainer - Remember to keep all parts in Piston order for reassembly.
7.4 CHECKING THE FUNCTION It is to ensure that the pipette has been assembled correctly. Carry out a gravimetric test for the systematic and random error. 7.5 DISASSEMBLING THE MULTI-CHANNEL PIPETTE 7.5.1 REMOVING THE LOWER ASSEMBLY 1. Press the tip ejector pusher completely and hold it while unscrewing the coupler from upper part of pipette.
7.8 AUTOCLAVING This pipette is completely autoclavable at 1 bar pressure and 121°C temperature for 20 minutes exposure time. AUTOCLAVING INSTRUCTIONS Keep digital counter in unlock position. Ÿ Do not dis-assemble the pipette for autoclaving. Ÿ After autoclaving, allow the pipette to completely cool and Ÿ...
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Avantor Performance Materials India Limited 17th Floor, Building No. 5, Tower C DLF Cyber City Phase - III, Gurgaon - 122002, Haryana...