VWR VWRA613-6688 Manual

VWR VWRA613-6688 Manual

Precise high performance

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PHP Pipette
Precise High Performance

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Summary of Contents for VWR VWRA613-6688

  • Page 1 PHP Pipette Precise High Performance...
  • Page 3: Table Of Contents

    TABLE OF CONTENTS Sr. No. Description Page # Introduction Operating instructions for using this manual Danger symbols and danger levels Intended use Package contents Product description Variable volume pipette range Fixed volume pipette range Multi channel pipette range Setting the volume Volume indicator display example Materials Pipette operation...
  • Page 4: Introduction

    INTRODUCTION You are now the proud owner of one of the most precise and lowest plunging force required mechanical pipette. This manual will guide you on how to take care of your pipette and benefit the most from its advanced features as follows: •...
  • Page 5 SINGLE CHANNEL VARIABLE VOLUME Volume adjustment knob Volume lock Tip ejector pusher Volume range Coupler Tip ejector Tip cone - 2 -...
  • Page 6 MULTICHANNEL VARIABLE VOLUME Volume adjustment knob Volume lock Tip ejector pusher Volume range Coupler Manifold Tip cone - 3 -...
  • Page 7: Variable Volume Pipette Range

    CAT No. Code [ μl ] [ μl ] [ μl ] (±) % (±) % 0.25 0.1-2.5 μl 0.002 VWRA613-6688 1.25 0.5-10 μl 0.02 VWRA613-6689 2-20 μl 0.02 VWRA613-6690 5-50 μl VWRA613-6691 10-100 μl VWRA613-6692 20-200 μl VWRA613-6693 100-1000 μl...
  • Page 8: Multi Channel Pipette Range

    Volume Test Inac- Impreci- Color Range Volume curacy sion CAT No. Code [ μl ] [ μl ] (±) % (±) % 250 μl VWRA613-6705 500 μl VWRA613-6706 1000 μl 1000 VWRA613-6707 2000 μl 2000 VWRA613-6708 0.15 5000 μl 5000 VWRA613-6709 0.15 10000 μl...
  • Page 9: Volume Indicator Display Example

    4.5 VOLUME INDICATOR DISPLAY EXAMPLE VWRA613-6688 VWRA613-6689 VWRA613-6690 VWRA613-6692 2.25 μl 10 μl 18.3 μl 100 μl VWRA613-6694 VWRA613-6695 VWRA613-6696 VWRA613-6716 1 ml 4.85 ml 9.30 ml 300 μl 4.6 MATERIALS NOTICE! Aggressive substances may damage components, consumables and accessories.
  • Page 10: Setting The Volume

    • After pipetting acids or other corrosive liquids that emit vapors, remove the tip-cone and rinse the piston, O-ring and seal with distilled water. • Do not pipette liquids having temperatures above 70°C. (Not recommendable) • Make sure that liquids never enter the tip-cone. To prevent this: Avoid laying the pipette horizontally when tip is filled with sample.
  • Page 11: Loading Tips

    Setting the volume beyond the allowable volume range is not permitted. Using excessive force to turn the volume adjustment knob outside the permitted range will jam the mechanism, damage the pipette and void the warranty. 5.3 LOADING TIPS Using correct pipette tip according to the pipette volume range is recommended.
  • Page 12: Reverse Pipetting

    5.6 REVERSE PIPETTING The reverse pipetting technique is suitable for dispensing liquids that have high viscosity or a tendency to foam easily. This technique is also recommended for dispensing very small volumes. Press the operating knob until the second stop. Dip the tip under the surface of the liquid in the reservoir and slowly release the operating knob.
  • Page 13: Devise Requirements And Test Conditions

    6.1 DEVISE REQUIREMENTS AND TEST CONDITIONS An analytical balance must be used. The balance selection depends upon selected model of the pipette and sensitivity of balance reading. Test liquid: Water, distilled or de-ionized, grade 3 water conforming ISO3696 Calibration should be carried out in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C to 30°C.
  • Page 14: Conversion Of Weight Readings To Volume

    • Calculate the inaccuracy and imprecision for all three volumes as per EN ISO 8655-6 standards on the basis of the following calculation 6.3.1 CONVERSION OF WEIGHT READINGS TO VOLUME Mean Volume Mean Weight Balance Reading number of reading Conversion factor [example Z=1.0040 µl/mg at 25°c and 1013 hPa] 6.3.2 CALCULATION FOR IN-ACCURACY (SYSTEMATIC ERROR) Mean Value...
  • Page 15: Disassembling The Single Channel Pipette Up To 1Ml

    7.2.1 DISASSEMBLING THE SINGLE-CHANNEL PIPETTE UP TO 1ml 1. Unscrew the coupler and remove tip cone. Piston Spring 2. Remove the lower part and pull out the piston and other parts from tip cone. Retainer - Remember to keep all parts in Piston order for reassembly.
  • Page 16: Checking The Function

    7.4 CHECKING THE FUNCTION It is to ensure that the pipette has been assembled correctly. Carry out a gravimetric test for the systematic and random error. 7.5 DISASSEMBLING THE MULTI-CHANNEL PIPETTE 7.5.1 REMOVING THE LOWER ASSEMBLY 1. Press the tip ejector pusher completely and hold it while unscrewing the coupler from upper part of pipette.
  • Page 17: List Of Spare Parts - Single Channel Pipette

    7.6 LIST OF SPARE PARTS - SINGLE CHANNEL PIPETTE Group 1 Group 2 Variable volume 0.1-2.5µl Variable volume 0.5-10µl Fix volume 2.5µl Fix volume 10µl DP 030 DP 031 DP 0269 DP 0269 DP 036 DP 037 DP 0261 DP 0262 DP 0159 DP 0159 SP 0158...
  • Page 18 Group 3 Group 4 Variable volume 2-20µl Variable volume 5-50µl Fix volume 20µl Fix volume 25µl 50µl DP 033 DP 029 DP 0270 DP 0269 DP 0264 DP 039 DP 0263 DP 0361 DP 038 DP 0152 DP 0160 SP 0152 DP 023 DP 024 - 15 -...
  • Page 19 Group 5 Group 6 Variable volume 10-100µl Variable volume 20-200µl Fix volume 100µl Fix volume 200µl DP 035 DP 034 DP 0270 DP 0270 DP 041 DP 0266 DP 040 DP 0265 DP 0363 DP 0362 DP 0154 DP 0153 DP 0162 DP 0161 DP 026...
  • Page 20 Group 7 Variable volume 1 00 - 1000µl Fix volume 250µl 500µl 1000µl DP 34 DP 271 DP 042 DP 265 DP 0155 DP 0163 DP 027 - 17 -...
  • Page 21 Group 8 Group 9 Variable volume 0.5-5ml Variable volume 1-10ml Fix volume Fix volume 10ml DP 021 DP 020 DP 0268 DP 0268 DP 0273 DP 0272 SP 0168 SP 0168 SP 0044 SP 0060 SP 0162 RP 0156 SP 0108 SP 0110 DP 047 DP 048...
  • Page 22: List Of Spare Parts - Multichannel Pipette

    7.7 LIST OF SPARE PARTS - MULTICHANNEL PIPETTE Part Cat# VWRA613-6711 VWRA613-6712 VWRA613-6713 VWRA613-6714 VWRA613-6715 VWRA613-6716 VWRA613-6717 VWRA613-6718 VWRA613-6719 VWRA613-6720 VWRA613-6721 VWRA613-6722 (0.5-10µl) (2-20µl) (5-50µl) (10-100µl) (20-200µl) (30-300µl) DPM 165 DPM 165 DPM 165 DPM 165 DPM 165 DPM 165 DPM 134 DPM 135 DPM 136...
  • Page 23: Autoclaving

    7.8 AUTOCLAVING This pipette is completely autoclavable at 1 bar pressure and 121°C temperature for 20 minutes exposure time. AUTOCLAVING INSTRUCTIONS Keep digital counter in unlock position. Ÿ Do not dis-assemble the pipette for autoclaving. Ÿ After autoclaving, allow the pipette to completely cool and Ÿ...
  • Page 24 Avantor Performance Materials India Limited 17th Floor, Building No. 5, Tower C DLF Cyber City Phase - III, Gurgaon - 122002, Haryana...

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