Table of Contents
Advertisement
Setting the volume beyond the allowable volume range is
not permitted. Using excessive force to turn the volume
adjustment knob outside the permitted range will jam the
mechanism, damage the pipette and void the warranty.
5.3 LOADING TIPS
Using correct pipette tip according to the pipette volume range
is recommended. It is necessary to check that the tip cone is
clean before fitting a tip. Press the tip on the cone of the pipette
firmly to ensure an air tight seal. Always ensure that the tip is
correctly sealed to avoid any leakage while pipetting.
5.4 OPTIMUM IMMERSION DEPTHS
Volume
0.1 μl - 1 μl
1 μl - 100 μl
100 μl - 1000 μl
1ml - 10ml
Tip immersion depth is critical and should not be exceeded, as
then the volume measured may be inaccurate, possibly out of
specification. The tip angle is also important; the pipette should
always be used in a position within 20° angle of vertical. See
figure below.
TIP
5.5 FORWARD PIPETTING
1.
Press the operating knob until the first stop.
2.
Dip the tip under the surface of the liquid in the reservoir
and slowly release the operating knob. Withdraw the tip
from the liquid, touching it against the edge of the
reservoir to remove excess liquid.
3. Dispense the liquid by gently
pressing the operating knob to the
first stop. After a delay of about
one second, continue to press the
operating knob all the way to the
second stop. This action will empty
the tip.
4. Release the operating button to the
ready position. Change the tip and
continue pipetting.
Immersion depth
2 - 3 mm
2 - 4 mm
3 - 5 mm
IMMERSION DEPTH
- 8 -
1 mm
TIP
ANGLE
20°
Advertisement
Table of Contents
