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A
B
A. To enable volume selection, set the adjustment knob
to the upper position.
B. To set a certain value, turn the knob. The volume is
indicated by the counter and its value consists of
four digits that should be read from top to bottom.
Digits on the bottom drum represent the scale divi-
sion for the pipettor model. See scale intervals in
Section 5. The volume range of the pipettor model is
shown on the counter overlay.
C. After the desired volume has been set, move the
knob down to avoid an inadvertent change of the
volume.
Examples of counter indications
The black
mark sepa-
rates the
integer part
from the deci-
mal part of
the aspirated
liquid volume
in μL.
Volume Ranges
0.1-2 µL
Set Volume
0.1 µL
To attain the maximum accuracy, the set volume must
be approached from a higher value by diminishing
counter readings. Before reaching the desired value,
reduce the speed of turning the adjustment knob to not
exceed this value.
7. Operating Instructions
Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid
sampling.
10 – VWR UHP PIPETTORS
C
5-50 µL
100-1,000 µL
5 µL
100 µL
• During operation, hold the adjustment knob to protect
it against rotation.
• The pipettor is equipped with a counter operating
mode switch, the calibration switch located under the
CAL sign on the counter overlay. This switch should
only be used when the pipettor is calibrated (upper
position marked as CAL on the switch).
• Make sure to operate the pipettor slowly and
smoothly.
• The depth of immersion in the sample liquid should
be the minimum necessary and should remain con-
stant during aspiration. The recommended immersion
depths are given in the table below:
Model Volume Range [µL]
0.1-1
1-100
101-1,000
• The pipettor should be held in a vertical position.
• Change the pipet tip when volume setting is changed
or when a different liquid is to be aspirated.
• Change the pipet tip if a droplet remains on the end
of the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid
to be pipetted.
• Liquid should never enter the pipettor shaft.
To prevent this:
– Press and release the pushbutton slowly and
smoothly.
– Never turn the pipettor upside down.
– Never lay the pipettor on its side when there is
liquid in the tip.
• Never force the volume setting beyond its recom-
mended limits.
When pipetting liquids with temperatures different from
the ambient temperature, it is recommended to pre-
rinse the tip several times before use.
Do not pipette liquids with temperatures above 70°C.
NOTE: When pipetting acids or corrosive solutions
which emit vapors, it is recommended to disassemble
the shaft and rinse the plunger and seal with distilled
water after finishing the pipetting operation.
Immersion Depth [mm]
≤1
2-3
2-4
Instruction Manual – 11
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