GE Biacore X100 Operating Instructions Manual page 80

7 Troubleshooting
7.1 Instrument related problems
Failed test
A: Peristaltic
pump calibration
B: Response
C: Injections
D: Noise
Liquid flow problems
Problem
Buffer is not
suppliedproper-
ly.
80
Action
Check that the pump cover is properly closed.
Check that the buffer tubes reach the buffer liquid and that they
are not squeezed.
Check that the pump tube does not leak and has not been
punctured by mistake.
If the test fails repeatedly, change the pump tube.
If the Fc-values in the results are too low, check that you used
a non-immobilized Sensor Chip CM5.
If the Fc-values in the result are too low or too high, check that
you used the correct buffer (HBS-EP+ buffer) and test solution
(BIAtest solution).
Check that the tubes do not leak.
Contact your local GE service representative.
Check that you used a non-immobilized Sensor Chip CM5.
Check that the temperature stability is not affected by inade-
quate ventilation, exposure to direct sunlight or similar.
Possible cause
There is not enough
buffer in the buffer bot-
tle.
The cover of the peri-
staltic pump is not prop-
erly closed.
The flow system is leak-
ing.
Action
Ensure that there is enough buffer be-
fore starting the run.
Close the cover.
Check for leaks (CheckLeaks).
Biacore X100 Operating Instructions 28961142 AD