Sigma 6-16KS Operating Manual
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Sigma 6-16KS Operating Manual

Refrigerated centrifuge
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Translation of the original operating manual
sb
06/2012
Rev. 1.11 of 06/04/2016
Sigma 6-16KS; ;
part no. 0702202
Sigma 6-16KS
from serial no. 146304
Refrigerated Centrifuge
Operating Manual
Please retain for later use!

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Summary of Contents for Sigma 6-16KS

  • Page 1 Translation of the original operating manual 06/2012 Rev. 1.11 of 06/04/2016 Sigma 6-16KS; ; part no. 0702202 Sigma 6-16KS from serial no. 146304 Refrigerated Centrifuge Operating Manual Please retain for later use!
  • Page 2 Refrigerated Centrifuge Sigma 6-16KS Version 06/2012, Rev. 1.11 of 06/04/2016 • sb 2 / 91 Translation of the original operating manual, part no. 0702202...
  • Page 3 Refrigerated Centrifuge Sigma 6-16KS In case of inquiries, please state the following numbers: Order number: Serial number: © Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Tel.: +49 (0) 5522 / 5007-0 Fax: +49 (0) 5522 / 5007-12 Web: www.sigma-zentrifugen.de...
  • Page 4 Refrigerated Centrifuge Sigma 6-16KS Version 06/2012, Rev. 1.11 of 06/04/2016 • sb 4 / 91 Translation of the original operating manual, part no. 0702202...

  • Page 5: Table Of Contents

    Refrigerated Centrifuge Sigma 6-16KS Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability......................9 Copyright ........................10 Standards and regulations ....................10 Scope of Supply ......................10 Layout and mode of operation ..................11 Layout of the centrifuge ....................
  • Page 6 Refrigerated Centrifuge Sigma 6-16KS Table of contents Storage and transport ....................... 24 Dimensions and weight ....................24 Storage conditions ......................24 Notes on transport ......................24 Packaging ........................25 Transport safety device ....................25 Set-up and connection ...................... 26 Installation site ........................ 26 Power supply ........................
  • Page 7 Refrigerated Centrifuge Sigma 6-16KS Table of contents 6.3.3 Program mode ......................52 6.3.3.1 Saving a program ....................53 6.3.3.2 Loading a program ..................... 53 6.3.3.3 Executing a program ..................54 6.3.3.4 Deleting a program .................... 54 6.3.3.5 Automatic program rotation ................55 6.3.4...
  • Page 8 Refrigerated Centrifuge Sigma 6-16KS Table of contents Version 06/2012, Rev. 1.11 of 06/04/2016 • sb 8 / 91 Translation of the original operating manual, part no. 0702202...

  • Page 9: General Information

    Pos : 1 /100 Sigma/100 BA Z entrifug en Sig ma ( Standardmodule) /010 Allgemeine Infor mationen010 Allgemei ne Informati onen ==================================== @ 26\mod_1405318948131_68.doc x @ 191680 @ 1 @ 1 General information Pos : 2 /100 Sigma/100 BA Z entrifug en Sig ma ( Standardmodule) /010 Allgemeine Infor mationen010- 0010 Stellenwert der Betriebs anl eitung------------------------------------------------------- @ 26\mod_1405318949962_68.doc x @ 191694 @ 2 @ 1 Importance of the operating manual...

  • Page 10: Copyright

    Non-compliance may be prosecuted under criminal law. Pos : 9 /100 Sigma/100 BA Z entrifug en Sig ma ( Standardmodule) /010 Allgemeine Infor mationen010- 0050 N or men und Vorsc hriften------------------------------------------------------ @ 26\mod_1405318958202_68.doc x @ 191765 @ 2 @ 1 →...

  • Page 11: Layout And Mode Of Operation

    Layout of the centrifuge Pos : 16 /100 Sigma/110 BA Zentrifugen Sigma (Pr ojekte) /6- 16KS_6-16KH S/020 Aufbau und Wir kungs weise020- 0010-0010 F unkti ons- und Bedi enel emente 6-16KS @ 29\mod_1405327175487_68.doc x @ 199255 @ 3 @ 1 2.1.1...

  • Page 12: Name Plate

    Pos : 17 /010 U ni vers al module/Leerz eile @ 0\mod_1202116244500_0.doc x @ 114 @ @ 1 Pos : 18 /100 Sigma/110 BA Zentrifugen Sigma (Pr ojekte) /6- 16KS_6-16KH S/020 Aufbau und Wir kungs weise020- 0010-0020 T ypenschil d 6-16KS @ 29\mod_1405327178393_68.doc x @ 199297 @ 3 @ 1 2.1.2...

  • Page 13: Mode Of Operation

    Pos : 20 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/020 Aufbau und Wir kungs weise020- 0020 Wir kungs weis e---------------------------------------------------------- @ 26\mod_1405318984194_68.doc x @ 191807 @ 2 @ 1 → Mode of operation Pos : 21 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/020 Aufbau und Wir kungs weise020- 0020-0010 Pri nzip der Z entrifug ation @ 26\mod_1405318985448_68.doc x @ 191821 @ 3 @ 1 → 2.2.1...

  • Page 14: Speed, Radius, And Relative Centrifugal Force

    2 Layout and mode of operation Pos : 25 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/020 Aufbau und Wir kungs weise020- 0020-0020-0010 Dr ehz ahl, R adi us, Rel ati ve Z entrifug albesc hleunig ung @ 26\mod_1405318988082_68.doc x @ 191849 @ 4 @ 1 →...

  • Page 15: Safety

    Pos : 30 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030 Sicher heit======================================= @ 26\mod_1405319007254_68.doc x @ 191877 @ 1 @ 1 → Safety Pos : 31 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030- 0010 Besc hilderung des Geräts (auß er 3- 30KS) @ 30\mod_1405404002477_68.doc x @ 203921 @ 2 @ 1 → Marking of the unit...

  • Page 16: Explanation Of The Symbols And Notes

    Refrigerated Centrifuge Sigma 6-16KS 3 Safety Explanation of the symbols and notes In this operating manual, the following names and symbols to indicate hazards are used: This symbol stands for a direct hazard to the life and health of persons.

  • Page 17: Responsibility Of The Operator

    Pos : 38 /010 U ni vers al module/Leerz eile @ 0\mod_1202116244500_0.doc x @ 114 @ @ 1 Pos : 39 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030- 0050 Infor melle Sic her hei tshi nwei se @ 26\mod_1405319019966_68.doc x @ 192017 @ 2 @ 1 →...

  • Page 18: Safety Instructions

    Pos : 43 /010 U ni vers al module/Leerz eile @ 0\mod_1202116244500_0.doc x @ 114 @ @ 1 Pos : 44 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030- 0060-0020 Mechanische Sic her heit (außer 1- 16/1- 16K) @ 26\mod_1405319024314_68.doc x @ 192059 @ 3 @ 1 →...

  • Page 19: Fire Prevention

    Pos : 45 /010 U ni vers al module/Leerz eile @ 0\mod_1202116244500_0.doc x @ 114 @ @ 1 Pos : 46 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030- 0060-0030 Br andsc hutz ther misc he Sic her ungen @ 26\mod_1405319025698_68.doc x @ 192073 @ 3 @ 1 →...

  • Page 20: Safety Instructions For Centrifugation

    Refrigerated Centrifuge Sigma 6-16KS 3 Safety Pos : 50 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030- 0060-0050 Sic herheits hinweis e z ur Zentrifugation @ 26\mod_1405319029632_68.doc x @ 192115 @ 3 @ 1 → 3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: •...

  • Page 21: Service Life Of Rotors And Accessories

    Refrigerated Centrifuge Sigma 6-16KS 3 Safety Pos : 54 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030- 0060-0070 Lebens dauer von Rotor en und Z ubehör @ 26\mod_1405319032612_68.doc x @ 192143 @ 3 @ 1 3.6.7 Service life of rotors and accessories The rotors and accessories have a limited service life.

  • Page 22: Safety Devices

    Pos : 62 /010 U ni vers al module/Leerz eile 8pt @ 12\mod_1337063062114_0.doc x @ 65687 @ @ 1 Pos : 63 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030- 0070-0040 Schutzl eiterprüfung Spi nc ontr ol L+S+ Eas y+U ni versal @ 27\mod_1405319043129_68.doc x @ 192241 @ 3 @ 1 →...

  • Page 23: Measures In The Event Of Hazards And Accidents

    Refrigerated Centrifuge Sigma 6-16KS 3 Safety Pos : 71 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit030- 0080 Verhalten bei Gefahren und U nfällen @ 27\mod_1405319051188_68.doc x @ 192325 @ 2 @ 1 → Measures in the event of hazards and accidents •...

  • Page 24: Storage And Transport

    Storage and transport Pos : 77 /100 Sigma/110 BA Zentrifugen Sigma (Pr ojekte) /6- 16KS_6-16KH S/040 Lagerung und Tr ans port040-0010 Abmess ungen und Gewic ht 6- 16KS @ 29\mod_1405327204443_68.doc x @ 199325 @ 2 @ 1 Dimensions and weight...

  • Page 25: Packaging

    Pos : 84 /010 U ni vers al module/Leerz eile @ 0\mod_1202116244500_0.doc x @ 114 @ @ 1 Pos : 85 /100 Sigma/110 BA Zentrifugen Sigma (Pr ojekte) /6- 16KS_6-16KH S/040 Lagerung und Tr ans port040-0050 Tr ansportsic her ung 6-16KS_KH S - Ü berschrift @ 29\mod_1405327206740_68.doc x @ 199367 @ 2 @ 1 Transport safety device Pos : 86 /100 Sigma/110 BA Zentrifugen Sigma (Pr ojekte) /6- 16KS_6-16KH S/040 Lagerung und Tr ans port/040-0051 Trans portsic herung 6- 16KS_KHS - Text @ 36\mod_1434606799956_68.doc x @ 264022 @ @ 1...

  • Page 26: Set-Up And Connection

    Pos : 92 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/050 Aufstellung und Ans chl uss 050-0020 Energieversorgung------------------------------------------------------ @ 27\mod_1405319175630_68.doc x @ 192409 @ 2 @ 1 → Power supply Pos : 93 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/050 Aufstellung und Ans chl uss/050- 0020-0012 Ansc hl uss art (Festkabel+Sc hutzs chalter) @ 7\mod_1306312098369_68.doc x @ 43506 @ 3 @ 1 → 5.2.1...

  • Page 27: Special Equipment: Water Cooling System

    5 Set-up and connection Pos : 97 /100 Sigma/110 BA Zentrifugen Sigma (Pr ojekte) /6- 16KS_6-16KH S/050 Aufstellung und Ans chl uss 050-0050 Sonderausstattung: Wass er kühlung KS_KH S @ 29\mod_1405327221757_68.doc x @ 199409 @ 2 @ 1 Special equipment: water cooling system...

  • Page 28: Using The Centrifuge

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 100 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S060 Betri eb========================================= @ 27\mod_1405319198895_68.doc x @ 192423 @ 1 @ 1 → Using the centrifuge Pos : 101 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S060-0010 Erste Inbetriebnahme @ 27\mod_1405319200130_68.doc x @ 192437 @ 2 @ 1...

  • Page 29: Installation Of Angle Rotors With A Hermetically Sealed Lid

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge When using rotors for microtiter plate formats: Ensure that the plate holders are inserted together with the plates into the buckets. CAUTION The lid screw serves for the fastening of the lid onto the rotor only, not for the fastening of the rotor onto the motor shaft.

  • Page 30: Installation Of Accessories

    6 Using the centrifuge Pos : 112 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S060-0020- 0020-0032 Ei ns etz en von Zubehör 4+6plätzig 4- 16S bis 6-16KS @ 27\mod_1405319207709_68.doc x @ 192535 @ 4 @ 1 →...

  • Page 31: Adapters

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Centrifugation with low capacity • Install the tubes axial symmetrically so that the buckets and their inserts are loaded evenly. • It is not permissible to load angle rotors on only one axis.

  • Page 32: Tubes

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 116 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S060-0020- 0020-0050 Gefäß e (auß er 4-5L) @ 27\mod_1405319211622_68.doc x @ 192577 @ 4 @ 1 →...

  • Page 33: Centrifugation With Tubes For Mineral Oil

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge °C 3,750 4,544 20 (2 min) Full blood 3,570 4,544 20 (2 min) Full blood in 4fold bags 4,000 5,170 24 (3 min) Plasma with buffy coat 3,750 4,544 20 (2 min)

  • Page 34: Control System "Spincontrol S

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 122 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030 Spinc ontrol S Steuerung---------------------------------------------- @ 27\mod_1405320192364_68.doc x @ 193109 @ 2 @ 1 →...

  • Page 35: Manual Mode

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 125 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0020 Manueller Betrieb @ 27\mod_1405320198028_68.doc x @ 193151 @ 3 @ 1 →...

  • Page 36: Standard Menu

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 134 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0020-0050 M enü Standard - nur KS @ 27\mod_1405320204084_68.doc x @ 193221 @ 4 @ 1 →...
  • Page 37 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Runtime The set runtime is displayed in the upper section of this field, with the remaining runtime shown below. The runtime is defined as the period from the start of the centrifuge to the beginning of the deceleration phase. The maximum value is 99 h 59 min 59 sec.
  • Page 38 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Rotor: rotor selection list This field shows the rotor that is currently being used. • Select the field "Rotor" and confirm the selection. A list with all of the possible rotors without buckets is displayed.
  • Page 39 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Acceleration This function is used to select an acceleration curve. One can select a linear rise (curves 0-9) or a quadratic rise (curves 10-19). The acceleration curves 20-29 can be programmed as desired (see chapter 11.3 - "Acceleration and deceleration curves").
  • Page 40 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Fig. 17: Program "RAPID_TEMP" The program will only be loaded if the actual temperature is above the set temperature. NOTE • Press the start button in order to start the rapid cooling run.
  • Page 41 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge If the "RAPID_TEMP" program is used, the temperature of the unloaded aluminium bucket will be displayed. If samples, which have not been precooled, are placed into the buckets, the displayed temperature will deviate from the actual sample temperature.

  • Page 42: Process Library Menu

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 136 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0020-0055 M enü Proz ess bibli othek @ 27\mod_1405320209123_68.doc x @ 193276 @ 4 @ 1 6.3.2.6...

  • Page 43: Parameters Menu

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 138 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0020-0060 M enü Par ameter - nur KS @ 27\mod_1405320218640_68.doc x @ 193305 @ 4 @ 1 →...
  • Page 44 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Density This setting is useful for glass vessels. If the density of the liquid to be centrifuged is higher than 1.2 g/cm , the value must be adapted manually in order to prevent the glass vessel from breaking. This will reduce the maximum possible final speed (see chapter 2.2.2.2 - "Density").
  • Page 45 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Temperature monitoring The control system includes a temperature monitoring function. If the actual temperature difference with regard to the set value deviates from the set temperature difference, the centrifuge will stop and an error message will be issued.
  • Page 46 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Lock In order to prevent any unauthorised use of the centrifuge, the following functions can be blocked: • Saving of programs (level 01) • Changing of parameters, short run (level 02) •...

  • Page 47: Setup Menu

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 140 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0020-0070 M enü Setup @ 27\mod_1405320225479_68.doc x @ 193361 @ 4 @ 1 →...
  • Page 48 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Start delay If the start delay function is active, the centrifuge will not start until the preset time has elapsed. The symbol " " will be displayed in the runtime field. Display deceleration time In the activated mode, the deceleration time will be displayed instead of the runtime during and after the deceleration process.
  • Page 49 Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge System Cycles This field shows the number of cycles as well as the runtime of the rotor and buckets that are used. Info This item provides information on the software versions that are used in this centrifuge.

  • Page 50: Curve Menu

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 142 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0020-0080 M enü Kur ve @ 27\mod_1405320228169_68.doc x @ 193389 @ 4 @ 1 →...

  • Page 51: 6.3.2.10 Help Menu

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Restrictions • Acceleration and deceleration curves can include intervals with a positive slope as well as intervals with a negative slope and also intervals with 0 slope. • The slope of the curve intervals can be 1 min...

  • Page 52: 6.3.2.11 Changing The Contrast

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 146 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0020-0100 Kontr aständerung @ 27\mod_1405320232376_68.doc x @ 193431 @ 4 @ 1 →...

  • Page 53: Saving A Program

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 150 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0030-0010 Programm s peichern @ 27\mod_1405320237832_68.doc x @ 193487 @ 4 @ 1 →...

  • Page 54: Executing A Program

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Fig. 28: Loading a program Pos : 153 /010 Uni vers almodul e/Leerz eile @ 0\mod_1202116244500_0.doc x @ 114 @ @ 1 Pos : 154 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0030-0030 Programm ausführ en @ 27\mod_1405320243642_68.doc x @ 193543 @ 4 @ 1 →...

  • Page 55: Automatic Program Rotation

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 158 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0030-0050 Automatisc he Pr ogrammrotati on @ 27\mod_1405320248329_68.doc x @ 193585 @ 4 @ 1 →...

  • Page 56: Options For Data Input And Output

    Refrigerated Centrifuge Sigma 6-16KS 6 Using the centrifuge Pos : 160 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7) 060-0030- 0040 Opti onen z ur Ei n- und Ausg abe von D aten (außer 8KS) @ 27\mod_1405320251483_68.doc x @ 193613 @ 3 @ 1 →...

  • Page 57: Malfunctions And Error Correction

    Refrigerated Centrifuge Sigma 6-16KS 7 Malfunctions and error correction Pos : 165 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/070 Störungen und F ehl ersuche070 Stör ung en und Fehl ersuc he============================= @ 28\mod_1405320576072_68.doc x @ 194663 @ 1 @ 1 →...

  • Page 58: Emergency Lid Release

    Refrigerated Centrifuge Sigma 6-16KS 7 Malfunctions and error correction Pos : 168 /100 Sig ma/110 BA Z entrifugen Sig ma (Proj ekte)/6-16KS_6- 16KHS/070 Stör ungen und F ehl ersuche070-0010-0010 Notentrieg elung @ 29\mod_1405327259063_68.doc x @ 199493 @ 3 @ 1 7.1.1...

  • Page 59: Table Of Error Codes

    Refrigerated Centrifuge Sigma 6-16KS 7 Malfunctions and error correction Pos : 170 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/070 Störungen und F ehl ersuche070-0020 T abelle der F ehl er mel dungen @ 28\mod_1405320582996_68.doc x @ 194761 @ 2 @ 1 →...

  • Page 60: Service Contact

    Refrigerated Centrifuge Sigma 6-16KS 7 Malfunctions and error correction Pos : 172 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/070 Störungen und F ehl ersuche070-0030 Kontakt im Ser vicefall @ 28\mod_1405320584361_68.doc x @ 194775 @ 2 @ 1 →...

  • Page 61: Maintenance And Service

    Refrigerated Centrifuge Sigma 6-16KS 8 Maintenance and service Pos : 175 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/080 War tung und Instandhaltung080 Wartung und Instandhaltung========================= @ 28\mod_1405320612731_68.doc x @ 194789 @ 1 @ 1 → Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress.

  • Page 62: Condenser (Only Refrigerated Centrifuges)

    Refrigerated Centrifuge Sigma 6-16KS 8 Maintenance and service Pos : 180 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/080 War tung und Instandhaltung080-0010-0010- 0010 Kondens ator @ 28\mod_1405320616708_68.doc x @ 194831 @ 4 @ 1 → 8.1.2 Condenser (only refrigerated centrifuges) In order to cool the refrigerant that is compressed by the refrigeration unit, a lamellar condenser is used.

  • Page 63: Plastic Accessories

    Refrigerated Centrifuge Sigma 6-16KS 8 Maintenance and service Pos : 184 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/080 War tung und Instandhaltung080-0010-0020- 0010 Kuns tstoffzubehör @ 28\mod_1405320619602_68.doc x @ 194859 @ 4 @ 1 → 8.1.3.1 Plastic accessories The chemical resistance of plastic decreases with rising temperatures (see chapter 11.5 - "Resistance data").

  • Page 64: Glass Breakage

    Refrigerated Centrifuge Sigma 6-16KS 8 Maintenance and service Pos : 190 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/080 War tung und Instandhaltung080-0010-0060 Glas bruch @ 28\mod_1405320623434_68.doc x @ 194901 @ 3 @ 1 → 8.1.6 Glass breakage In the case of glass breakage, immediately remove all glass particles (e.g.

  • Page 65: 8.2.1 Autoclaving

    Refrigerated Centrifuge Sigma 6-16KS 8 Maintenance and service Pos : 194 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/080 War tung und Instandhaltung080-0020-0010 Autokl avi eren @ 28\mod_1405320627440_68.doc x @ 194943 @ 3 @ 1 → 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use.

  • Page 66: Service

    Refrigerated Centrifuge Sigma 6-16KS 8 Maintenance and service Pos : 196 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/080 War tung und Instandhaltung080-0030 Instandhal tungsar bei ten @ 28\mod_1405320628810_68.doc x @ 194957 @ 2 @ 1 → Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury.

  • Page 67: Return Of Defective Parts

    Refrigerated Centrifuge Sigma 6-16KS 8 Maintenance and service Pos : 198 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/080 War tung und Instandhaltung080-0040 R üc ks endung defekter Teil e @ 28\mod_1405320630226_68.doc x @ 194971 @ 2 @ 1 →...
  • Page 68 NOTE The forms can be downloaded online from www.sigma-zentrifugen.de → [Service] → [Overhaul and repair]. Pos : 199 /010 Uni vers almodul e/Abs chnitts wec hs el @ 0\mod_1202124514062_0.doc x @ 418 @ @ 1 Pos : 200 /010 Uni vers almodul e/Seitenwechs el @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1 Version 06/2012, Rev.

  • Page 69: Disposal

    Pos : 203 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/090 Entsorgung090-0010 Ents orgung der Z entrifug e---------------------------------------------- @ 28\mod_1405320660029_68.doc x @ 194999 @ 2 @ 1 → Disposal of the centrifuge In accordance with the directive 2002/96/EC, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household waste.

  • Page 70: 10 Technical Data

    Refrigerated Centrifuge Sigma 6-16KS 10 Technical data Pos : 208 /100 Sig ma/110 BA Z entrifugen Sig ma (Proj ekte)/6-16KS_6- 16KHS/100 T ec hnisc he D aten100 T ec hnische D aten 6- 16KS============================== @ 29\mod_1405327277386_68.doc x @ 199507 @ 1 @ 1...

  • Page 71: Ambient Conditions

    Refrigerated Centrifuge Sigma 6-16KS 10 Technical data Pos : 210 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/100 T ec hnisc he D aten100-0010 U mg ebungs bedi ngungen gekühl t @ 28\mod_1405320679042_68.doc x @ 195027 @ 2 @ 1 →...

  • Page 72: 11 Appendix

    Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Pos : 215 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/110 Anhang110 Anhang===================================== @ 28\mod_1405320699310_68.doc x @ 195069 @ 1 @ 1 → 11 Appendix Pos : 216 /100 Sig ma/110 BA Z entrifugen Sig ma (Proj ekte)/6-16KS_6- 16KHS/110 Anhang110-0010 Z ubehörprogramm 6- 16KS_KHS @ 29\mod_1405327288120_68.doc x @ 199535 @ 2 @ 1 11.1...
  • Page 73 Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 12130 Angle rotor 30 x 1.5/2.0 ml, for e.g. reaction vials no. 15008, 15040, incl. hermetic aluminium lid no. 17844 Radius max. 10.0 cm, radius min. 6.7 cm, angle 45°...
  • Page 74 Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 13220 Bucket for microtiter plates, permissible data with rotor 11150: Radius corner 16.6 cm, 4 100 3 120 Radius max. 16.0 cm, 3 007 Radius min.
  • Page 75 Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 13627 Polypropylene adapter for standard blood bags, 1 set = 2 pcs. 13635 Aluminium bucket for carriers for culture tubes no. 18604 - 18607, 1 set = 2 pcs.

  • Page 76: 11.1.1 Maximum Speed For Tubes

    Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Pos : 218 /100 Sig ma/110 BA Z entrifugen Sig ma (Proj ekte)/6-16KS_6- 16KHS/110 Anhang110-0010-0010 H öc hstdrehz ahl en von Gefäßen 6-16KS_KH S @ 29\mod_1405327289170_68.doc x @ 199549 @ 3 @ 1 11.1.1 Maximum speed for tubes...

  • Page 77: Speed-Gravitational-Field-Diagram

    Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Pos : 222 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/110 Anhang110-0020 Drehz ahl- Schwerefel d-Diagramm @ 28\mod_1405320705642_68.doc x @ 195153 @ 2 @ 1 → 11.2 Speed-gravitational-field-diagram Fig. 36: Speed-gravitational-field-diagram Pos : 223 /010 Uni vers almodul e/Seitenwechs el @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1 Version 06/2012, Rev.

  • Page 78: Acceleration And Deceleration Curves

    Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Pos : 224 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/110 Anhang110-0031 Besc hl eunigungs- und Br ems kur ven Spi nc ontrol S @ 28\mod_1405320708834_68.doc x @ 195181 @ 2 @ 1 →...
  • Page 79 Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Linear Curves 98 7 6 5 4 1000 curve 0 curve 1 curve 2 curve 3 curve 4 curve 5 curve 6 curve 7 curve 8 curve 9 Time [sec] Fig. 39: Diagram of linear curves...

  • Page 80: Table Of The Service Life Of Rotors And Accessories

    Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Pos : 226 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/110 Anhang110-0040 T abelle: Lebens dauer von Rotor en und Zubehör @ 28\mod_1405320709911_68.doc x @ 195195 @ 2 @ 1 → 11.4...

  • Page 81: Resistance Data

    Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Pos : 228 /100 Sig ma/100 BA Z entrifugen Sig ma (Standardmodul e)/110 Anhang110-0050 Bes tändig keitstabell e @ 28\mod_1405320711439_68.doc x @ 195209 @ 2 @ 1 → 11.5 Resistance data The data refer to resistance at 20°C.
  • Page 82 Refrigerated Centrifuge Sigma 6-16KS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate CuSO x 5H Cyclohexanol Decane...
  • Page 83 Refrigerated Centrifuge Sigma 6-16KS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate Methyl alcohol Methyl benzene Methyl ethyl ketone...
  • Page 84 Refrigerated Centrifuge Sigma 6-16KS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide NaOH Sodium sulfate Spirits Styrene...

  • Page 85: Ec Declaration Of Conformity

    Refrigerated Centrifuge Sigma 6-16KS 11 Appendix Pos : 230 /100 Sig ma/110 BA Z entrifugen Sig ma (Proj ekte)/6-16KS_6- 16KHS/110 Anhang110-0060 Konfor mitäts er klärung 6-16KS_KH S @ 29\mod_1405327290165_68.doc x @ 199563 @ 2 @ 1 11.6 EC declaration of conformity Version 06/2012, Rev.
  • Page 86 Refrigerated Centrifuge Sigma 6-16KS 11 Appendix === Ende der Liste für T extmar ke Inhalt === Version 06/2012, Rev. 1.11 of 06/04/2016 • sb 86 / 91 Translation of the original operating manual, part no. 0702202...

  • Page 87: 12 Index

    Refrigerated Centrifuge Sigma 6-16KS Index 12 Index Centrifugation of infectious, toxic, radioactive, or pathogenic substances ....... 19 Acceleration ..........39 Centrifugation principle ......13 Acceleration curve ......39, 50, 78 Centrifugation with different tube sizes ..30 Acceleration curve (changing) ....50 Centrifugation with low capacity ....
  • Page 88 Refrigerated Centrifuge Sigma 6-16KS Index Cycles ..........28, 49 Fire preventions ......... 19 Flow rate ............ 27 Damage of the surface ....... 63 Form for the return of defective parts ..67 Dangerous goods........20 Function ............. 47 Dangerous materials ........64 Functional and operating elements ....
  • Page 89 Refrigerated Centrifuge Sigma 6-16KS Index Invert ............48 IP code ............70 Name plate .......... 11, 12 No indication on the display ....... 57 No power in the mains supply ....57 Kinetic energy ..........70 Noise level ..........70 Non-greased bolts ........
  • Page 90 Refrigerated Centrifuge Sigma 6-16KS Index Screws of the transport safety device are not removed ..........57 Quadratic curves ........78 Selection, display, and modification of data 35 Quick stop ..........35 Serial number ........ 12, 60, 66 Service ............66 Radioactive substances .......
  • Page 91 Refrigerated Centrifuge Sigma 6-16KS Index System ............49 Type of the centrifuge ....60, 66, 70 System check ..........22 System error ........57, 59 Unblocking a function ......... 46 Ungreased load- bearing bolts ....57 Table of error codes ........59 Units of protection class I ......

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