Page 1
Operating Manual Refrigerated Centrifuge 8KBS Translation of the original operating manual from serial no. 143558 Version 08/2012 Rev. 1.6 of 04/02/2014 8KS; ;8KBS Please retain for later use!
Page 2
8KBS Version 08/2012, Rev. 1.6 of 04/02/2014 • sb Translation of the original operating manual...
8KBS Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability ......................9 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................10 Layout and mode of operation ..................11 Layout of the centrifuge ....................
Page 6
Table of contents Measures in the event of hazards and accidents ............24 Remaining hazards ......................24 Storage and transport ....................... 25 Dimensions and weight ....................25 Storage conditions ......................25 Notes on tranpsort ......................25 Packaging ........................26 Set-up and connection ...................... 27 Installation site ........................
Page 7
8KBS Table of contents 6.3.3 Program mode ......................53 6.3.3.1 Loading a program ..................... 53 6.3.3.2 Saving a program ....................54 6.3.3.3 Deleting a program .................... 55 6.3.3.4 Automatic program rotation ................55 6.3.3.5 Options for data input and output ............... 56 Switching the centrifuge off .....................
Page 8
Table of contents Version 08/2012, Rev. 1.6 of 04/02/2014 • sb Translation of the original operating manual...
They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. Sigma Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use.
Pos: 17 / 100 Sigma /110 BA Ze ntrifu gen (Proj ekte )/8KS_8KBS/02 0 Aufb au und Wi rkun gsweise /020 -0 010 -001 0 Fun ktions - u nd Bedi enel eme nte @ 7\ mod _130 743 276 924 7_6 8.doc...
Page 12
Pos: 18 / 010 Unive rsalm odul e/ Seit enwec hsel @ 0\m od_ 120 211 6244 312 _0. docx @ 10 5 @ @ 1 Not for Sigma 8KB Version 08/2012, Rev. 1.6 of 04/02/2014 • sb Translation of the original operating manual...
Pos: 26 / 100 Sigma /100 BA Ze ntrifu gen (Sta nda rdm odul e)/0 20 Auf bau und Wirku ngsweis e/02 0-0 020 -00 20 Anwen dun gsbe reich @ 7\ mod _13 068 365 440 60_ 68.d...
Pos: 36 / 100 Sigma /100 BA Ze ntrifu gen (Sta nda rdm odul e)/0 30 Sich erh eit/0 30- 0020 Sym bol- und Hi nweise rklär ung en @ 7\ mod _130 630 280 997 8_6 8.doc...
Pos: 88 / 100 Sigma /110 BA Ze ntrifu gen (Proj ekte )/8KS_8KBS/04 0 L age run g un d T rans por t/04 0-0 030 T rans po rthinw eise @ 7\m od_ 130 743 3087 618 _68 .docx...
Pos: 90 / 100 Sigma /110 BA Ze ntrifu gen (Proj ekte )/8KS_8KBS/04 0 L age run g un d T rans por t/04 0-0 040 Ver packu ng @ 7\ mod _130 743 315 676 8_6 8.doc...
Pos: 99 / 100 Sigma /110 BA Ze ntrifu gen (Proj ekte )/8KS_8KBS/05 0 Aufst ellun g un d Anschl uss/0 50- 001 1-0 010 Aufstell ung @ 7\ mod _13 074 331 575 49_6 8.d...
Page 29
8KBS 5 Set-up and connection • Open the lid with the emergency lid release system. To do so, lift off the plugs on the left side (e.g. with a screwdriver) and unlock the lid locks by turning them clockwise with the supplied square spanner (see chapter 7.1.1 - "Emergency lid release") •...
SIGMA laboratory centrifuges are units of safety class I. The centrifuges of the type 8KS and 8KBS have a five-wire power cord (2.5 m) with a 5- channel Cekon-plug (16 A). Behind the front door there are three fuses with a rocker switch.
Page 31
8KBS 5 Set-up and connection At the installation site, the condensate drain can be laid to the outside (see figure). NOTE Drain outside of the c entrifuge Hose (part no. 80415) Hose connector (unblocks the drain when connected) Unlocking button Plug (to be removed prior to the installation) Centrifuge door (front, left side)
8KBS 5 Set-up and connection Pos: 113 /10 0 Sigm a/11 0 BA Z entrif uge n (Pr ojekt e)/8KS_ 8KBS/050 Auf stellu ng u nd Ansc hluss/ 050 -00 60 So nde raus statt ung: Wasse rkühl ung @ 7\ mod _13 074 331 590 96_ 68.d ocx @ 442 83 @ 2 @ 1 Special equipment: water cooling system Centrifuges with water cooling are equipped with a special refrigeration unit with a refrigerant (CFC-free), modified electronic system, and a special...
Pos: 124 /10 0 Sigm a/11 0 BA Z entrif uge n (Pr ojekt e)/8KS_ 8KBS/060 Bet rieb /06 0-0 020 -00 20 Eins etze n von Roto ren un d Z ube hör 8KS @ 7\ mod _13 074 336 634 66_6 8.d...
8KBS 6 Using the centrifuge Pos: 132 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/06 0-0 020 -00 20- 004 0 Ad apte r - nur L+S @ 7\ mod _130 640 367 744 1_6 8.doc x @ 4 359 7 @ 4 @ 1 6.2.2.4 Adapters In order to ensure easy handling, even if vessels of various sizes are used,...
8KBS 6 Using the centrifuge Pos: 141 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0020 M anu eller B etrie b @ 1 0\m od_ 132 869 710 5069 _68 .docx @ 5 802 8 @ 3 @ 1 6.3.2 Manual mode Pos: 142 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0020 -0 010 Start en einer Ze ntrif uga tion @ 10\ mo d_13 286 971 053 03_ 68.d ocx @ 580 36 @ 4 @ 1...
8KBS 6 Using the centrifuge Pos: 150 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0020 -0 050 Me nü Sta nda rd -nu r KS @ 1 0\m od_1 328 697 106 319 _68. docx @ 58 068 @ 4 @ 1 6.3.2.5 Standard menu The standard menu is displayed a few seconds after the centrifuge has...
Page 41
8KBS 6 Using the centrifuge Runtime The set runtime is displayed in the upper section of this field, with the remaining runtime shown below. The runtime is defined as the period from the start of the centrifuge to the beginning of the deceleration phase. The maximum value is 99 h 59 min 59 sec.
Page 42
8KBS 6 Using the centrifuge Rotor: rotor selection list/ automatic rotor identification This field shows the rotor that is currently being used. • Select the field "Rotor" and confirm the selection. A list with all of the possible rotors without buckets is displayed. •...
Page 43
8KBS 6 Using the centrifuge Progr.: program selection list This field in the standard menu shows the program that is currently loaded. When the field is selected, the program list is displayed (for information on how to work with the programs, see chapter 6.3.3 - "Program mode"). The program "RAPID_TEMP"...
Page 44
8KBS 6 Using the centrifuge • Press the start button in order to start the rapid cooling process. • During the rapid cooling process, the set temperature can be modified within the range below the actual temperature. The current status of the program will be displayed on the status bar. The “RAPID_TEMP“...
8KBS 6 Using the centrifuge Pos: 152 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0020 -0 060 Me nü Pa ram ete r - nur KS @ 10\ mo d_13 303 392 655 11_ 68.d ocx @ 603 08 @ 4 @ 1 6.3.2.6 Parameters menu The "Parameters"...
Page 46
8KBS 6 Using the centrifuge Density This setting is useful for glass vessels. If the density of the liquid to be centrifuged is higher than 1.2 g/cm , the value must be adapted manually. This will reduce the maximum possible final speed (see chapter 2.2.2.2 - "Density").
Page 47
8KBS 6 Using the centrifuge Temperature monitoring The control system includes a temperature monitoring function. If the set temperature difference with regard to the set value deviates from the actual temperature difference, this function will stop the centrifuge and issue an error message.
8KBS 6 Using the centrifuge Unblocking a function • Select the button "Deactivate code". • Enter the code and confirm the entry. The function is now unblocked. Changing the code • Select the button "Change code". • Enter the old code and confirm the entry. •...
Page 49
8KBS 6 Using the centrifuge Runtime as of set speed If this function is active, the runtime will not be measured until the set speed is reached. The clock symbol " !" will be displayed in the runtime field. Start delay If the start delay function is active, the centrifuge will not start until the preset time has elapsed.
Page 50
8KBS 6 Using the centrifuge System Cycles This field shows the number of cycles as well as the runtime of the rotor and buckets that are currently being used. Info This menu provides information on the software versions that are used in this centrifuge.
8KBS 6 Using the centrifuge Pos: 157 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0020 -0 080 Me nü Ku rve @ 10\ mo d_13 286 971 065 69_ 68.d ocx @ 580 76 @ 4 @ 1 6.3.2.8 Curve menu In this menu, free acceleration and deceleration curves can be created and...
8KBS 6 Using the centrifuge Restrictions • Deceleration and acceleration curves may include intervals with a positive slope as well as with a zero slope. • The slope of the curve intervals may be between 1 rpm/sec and 1000 rpm/sec maximum. •...
8KBS 6 Using the centrifuge Pos: 161 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0020 -0 100 Kont rastä nde run g @ 10\ mod_ 132 869 710 760 1_6 8.doc x @ 5 810 8 @ 4 @ 1 6.3.2.10 Changing the contrast To change the contrast: •...
8KBS 6 Using the centrifuge Fig. 27: Loading a program Pos: 167 /01 0 Univ ersal mod ule/ Lee rzeile @ 0\ mo d_12 021 162 445 00_ 0.doc x @ 1 14 @ @ 1 Pos: 168 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0030 -0 021 Prog ra mm s peich ern - o hne Te mp @ 10\ mod _13 286 971 086 48_ 68.d ocx @ 581 40 @ 4 @ 1 6.3.3.2 Saving a program •...
8KBS 6 Using the centrifuge Pos: 170 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0030 -0 031 Prog ra mm lö sche n - ohn e T emp @ 1 0\mo d_1 328 697 108 898 _68. docx @ 58 148 @ 4 @ 1 6.3.3.3 Deleting a program •...
8KBS 6 Using the centrifuge While the program rotation is active, an arrow is displayed in the program field of the standard menu (see figure below). Fig. 31: Automatic program rotation Pos: 173 /01 0 Univ ersal mod ule/ Lee rzeile @ 0\ mo d_12 021 162 445 00_ 0.doc x @ 1 14 @ @ 1 Pos: 174 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060 Betrie b_Spinc ont rol L +S/Spinco ntr ol S ( Zent 7)/ 060 -00 30- 0030 -0 050 Opti one n zur Ein- und Aus gab e vo n Dat en @ 10\ mod _13 286 971 0946 1_6 8.d ocx @ 5816 4 @ 4 @ 1 6.3.3.5 Options for data input and output...
8KBS 7 Malfunctions and error correction 7.1.1 Emergency lid release In the event of a power failure, it is possible to manually open the centrifuge lid. • Switch off the mains power switch and disconnect the power cord from the socket. •...
8KBS 8 Maintenance and service Pos: 190 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 080 Wart ung und Inst andh altu ng/0 80 W art ung und Insta ndh altun g= === == == == === == == === == == = @ 7 \mo d_1 306 738 045 173_ 68. docx @ 43 806 @ 1 @ 1 Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress.
8KBS 8 Maintenance and service Pos: 195 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 080 Wart ung und Inst andh altu ng/0 80- 001 0-0 010 -00 10 Ko nde nsat or @ 7\ mod_ 130 674 197 144 8_6 8.docx @ 4 386 9 @ 4 @ 1 8.1.2 Condenser (only air-cooled centrifuges) In order to cool the refrigerant that is compressed by the refrigeration unit, a...
8KBS 8 Maintenance and service Pos: 205 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 080 Wart ung und Inst andh altu ng/0 80- 001 0-0 040 Glas bruc h @ 7\m od_1 306 741 772 069 _68. docx @ 43 862 @ 3 @ 1 8.1.6 Glass breakage In the case of glass breakage, immediately remove all glass particles (e.g.
8KBS 8 Maintenance and service Pos: 209 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 080 Wart ung und Inst andh altu ng/0 80- 002 0-0 010 Autokl avier en @ 7\m od_ 130 674 321 864 2_68 .docx @ 4 388 3 @ 3 @ 1 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use.
Please fill in the following forms completely, sign them, enclose them with the return package, and send them together with the product to: Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) 1. Declaration of decontamination...
Pos: 217 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 090 Entso rgun g/0 90- 001 0 Ents org ung de r Z entrif uge -- --- -- --- --- -- --- -- --- -- --- --- -- --- -- --- --- -- @ 7\ mod _13 067 454 030 22_6 8.d ocx @ 439 18 @ 2 @ 1 Disposal of the centrifuge In accordance with the directive 2002/96/EC, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household waste.
(e.g. circuit diagrams) and the safety data sheets of the manufacturers of refrigerant and lubricants are not attached to this documentation. You can order these documents at www.sigma-zentrifugen.de by way of our "service request" form. Pos: 227 /01 0 Univ ersal mod ule/ Ab schnitt swechs el @ 0 \mo d_1 202 124 514 062_ 0.d ocx @ 418 @ @ 1...
17752, 1 set = 2 pcs., service life max. 1.000 cycles Not for Sigma 8KBS Not for Sigma 8KBS Not for Sigma 8KBS incl. accessories Not for Sigma 8KBS incl. accessories Not for Sigma 8KBS Version 08/2012, Rev. 1.6 of 04/02/2014 • sb...
Page 72
Adapter for 1000 ml bottles Ø 98 mm no. 15920, 15921, 1 set = 2 pcs. The complete list of accessories can be downloaded from www.sigma-zentrifugen.de. Pos: 231 /01 0 Univ ersal mod ule/ Sei tenwe chsel @ 0\ mod _12 021 1624 431 2_0 .docx @ 1 05 @ @ 1 Version 08/2012, Rev.
Pos: 232 /10 0 Sigm a/11 0 BA Z entrif uge n (Pr ojekt e)/8KS_ 8KBS/110 An han g/11 0-0 010 -00 10 H öchst dre hzahl en v on G efä ßen 8KS @ 7\ mod _13 074 339 479 07_ 68.d...
8KBS 11 Appendix Pos: 238 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 110 Anha ng/1 10- 003 0 Besc hleu nigun gs- und B rem skurv en @ 7\ mod_ 130 683 683 832 4_6 8.docx @ 4 404 6 @ 2 @ 1 11.3 Acceleration and deceleration curves Linear as well as quadratic curves are numbered in the direction of...
8KBS 11 Appendix Pos: 242 /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 110 Anha ng/1 10- 005 0 Best ändi gkeitst abelle @ 7\ mo d_1 3073 432 578 92_ 68.d ocx @ 440 68 @ 2 @ 1 11.5 Resistance data The data refer to resistance at 20°C.
Page 79
8KBS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate CuSO x 5H Cyclohexanol Decane Diaminoethane Diesel fuel —...
Page 80
8KBS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Iodine, tincture of Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate Methyl alcohol Methyl benzene Methyl ethyl ketone (MEK) Methylene chloride Mineral oil...
Page 81
8KBS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Resorcinol Silver nitrate AgNO Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide NaOH Sodium sulfate Spirits Styrene Sulphuric acid Sulphuric acid...
Page 82
8KBS 11 Appendix Pos: 244 /10 0 Sigm a/11 0 BA Z entrif uge n (Pr ojekt e)/8KS_ 8KBS/110 An han g/11 0-0 060 Konf or mitäts erklä run g 8KS_8KBS @ 7 \mo d_1 307 433 9483 45_ 68. docx @ 44 442 @ 2 @ 1 Version 08/2012, Rev.
8KBS 11 Appendix Pos: 246 /10 0 Sigm a/11 0 BA Z entrif uge n (Pr ojekt e)/8KS_ 8KBS/110 An han g/11 0-0 070 Aufst ellzeich nung @ 7\ mo d_1 3074 339 486 88_ 68.d ocx @ 444 50 @ 2 @ 1 11.7 Layout plan here: water cooled centrifuge, GMP version, part no.
Page 86
8KBS 11 Appendix === E nde der Liste für Tex tma rke I nhal t = == Version 08/2012, Rev. 1.6 of 04/02/2014 • sb Translation of the original operating manual...
8KBS 12 Index 12 Index Centrifugation principle ......14 Centrifugation with different tube sizes ..35 Acceleration ..........44 Centrifugation with low capacity ....35 Acceleration curve ......44, 51, 75 Centrifuge cannot be started ...... 57 Accessories for blood bags ......71 Centrifuge decelerates during operation ..
Page 88
8KBS 12 Index Flow rate ............ 32 Form for the return of defective parts ..67 Damage of the surface ....... 63 Function ............. 48 Dangerous goods........21 Functional and operating elements .... 11 Dangerous materials ........64 Fuses ............30 Deactivating the help function ....
Page 89
8KBS 12 Index Notes on safety and hazards......9 Kinetic energy ..........69 Online download of forms ......67 Open lid after run ........48 Language ........... 49 Opening the lid ........... 33 Layout of the centrifuge......11 Operating conditions for water cooled Layout plan ..........
Page 90
8KBS 12 Index Service ............66 Service contact .......... 60 Radioactive substances ....... 20, 61 Service life ..........61 Radius ..........15, 45 Service life of rotors and accessories .. 22, 37, Range of accessories ........ 71 RAPID_TEMP program ......43 Service life of the accessories ....
Page 91
8KBS 12 Index Switching the centrifuge on ......33 Toxic substances ......... 20, 61 System ............50 Transport ........... 26 System check ..........23 Tubes ............36 System error ........57, 59 Type............69 Type of the centrifuge ......60, 66 Table Rotors and accessories with a different Unblocking a function .........
Need help?
Do you have a question about the 8KS and is the answer not in the manual?
Questions and answers