Biohit PROLINE PLUS Instruction Manual page 15

8. Read the weight in mgs (m
9. Repeat the test cycle until 10 measurements have been
recorded.
10. Convert the recorded masses (m
V = m Z
i i
11. Calculate the mean volume (
v̄
= (∑V )/10
i
12. For conformity evaluation calculate the systematic error e
the measurement:
in μl:
or in %:
13. For conformity evaluation calculate the random error of the
measurement:
as standard deviation s = ∑(V
or as coeffi cient of variation CV = 100
14. Compare the systematic error (inaccuracy) and random error
(imprecision) with the values in the performance specifi cations
- (p. 49) or the specifi cations of your own laboratory.
If the results fall within the specifi cations, the pipette is ready
for use. Otherwise check both systematic and random errors
and, when necessary, proceed to the recalibration procedure
(Chapter 11.2).
Note: Systematic error (inaccuracy) is the difference between the
dispensed volume and the selected test volume. Random error
(imprecision) is the scatter of the dispensed volumes around the
mean of the dispensed volume. (ISO 8655-1.)
Note: Biohit specifi cations are achieved in strictly controlled condi-
tions (ISO 8655-6). The user should establish own specifi cations
based on the fi eld of use and the accuracy requirements placed on
the pipette (ISO8655-1).
Table 1
Temp. (°C)
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
Note: This method is based on ISO 8655.
Z = correction factor (Table 1)
v̄
e = - V
s s
v̄
e = 100 (
s
Z-values (μl/mg):
Air Pressure (kPa)
95
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
).
i
) to volumes (V
i
v̄
) delivered:
V = selected test volume
s
- V )/V
s s
v̄
- ) n = number of
2
i
n - 1
/
s
100
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
)
i
measurement (10)
v̄
101.3
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
of
s
105
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
11