Biohit Proline Instruction Manual
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Biohit Proline Instruction Manual

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Proline
Käyttöohje
Bruksanvisning
Instruction Manual

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Summary of Contents for Biohit Proline

  • Page 1 ® Proline Käyttöohje Bruksanvisning Instruction Manual...
  • Page 2 Proline ® Käyttöohje ............1 Bruksanvisning .......... 17 Instruction Manual ........33 Specifications ..........48...
  • Page 3 Grip cover: Push button: Tip ejector: metal blue 721060 metal blue, 721059 metal blue, 721057 grey 721752 grey, 721751 grey, 721753 yellow 721825 white, 721223 yellow, 721826 721706 yellow, 721824 red, 721704 blue 721221 red, 721703 blue, 721230 green 721792 blue, 721220 green,...
  • Page 4 Coloured spare parts presented in 50-250 µl 721298 these two pages are for the metal 50-300 µl 721298 blue/gray Biohit Proline pipettors. Piston spring: Spare part codes for the white 0.5-10 µl 721261 handles and tip cone housings are 5-50 µl...

  • Page 5: Table Of Contents

    CONTENTS 1 . Your new Biohit Proline pipettor ............. 34 1.1. Biohit Proline adjustable volume pipettors........34 1.2. Biohit Proline fixed volume pipettors ...........35 1.3. H-sign (Konformitätsbescheinigung) ..........35 1.4. Biohit Proline tips ................ 36 Unpacking ..................37 Installing the pipettor holder ............37 Pipettor materials ................

  • Page 6: Your New Biohit Proline Pipettor

    All Biohit Proline pipettors operate on the basis of the air displacement principle and use disposable tips. The range of Biohit Proline pipettors cover a volume range from 0.1 µl to 5 ml. 1.1. Blohit Proline adjustable volume pipettors...

  • Page 7: Biohit Proline Fixed Volume Pipettors

    721005 721015 1.3. H-sign (Konformitätsbescheinigung) All Biohit Proline pipettors have been quality tested according to ISO 8655-6 and have received the H-sign (Konformitätsbescheinigung, DIN 12600) printed on the grip of each pipettor. The quality control according to ISO 8655 involves gravimetric testing of each pipettor with distilled water (grade 3, ISO 3696) at 22 C using the manufacturer’s...

  • Page 8: Biohit Proline Tips

    1.4. Biohit Proline tips The full range of Biohit pipettor tips are recommended for use with Biohit Proline pipettors. Biohit standard tips are made of virgin polypropylene. Biohit also offers a full range of filter tips. Biohit standard tips are available as bulk packages, space saving refill systems and autoclavable (121°C, 20 min, 1 atm) trays.

  • Page 9: Unpacking

    (Make sure the holder is pressed against the edge of the shelf.) 4. Place the pipettor onto the holder as shown in Figure 2B. Fig. 2A Fig. 3. Fig. 2B Note: An ergonomic, convenient carousel stand for 5 Biohit Proline pipettors is also available (Cat. No.721000) (Fig. 3).

  • Page 10: Pipettor Materials

    Pipettor Materials Biohit Proline Single Channel Push button Push button rod (ABS) (Operating button) (PA) Grip Cover (PA) Tip ejector (PA) Handle (ABS) Display cover (PC) Tip Ejector collar (PA) Biohit Proline Multichannel Tip cone Push button Push button 2.5–1000 µl (PVDF)

  • Page 11: Operating The Pipettor

    (Fig. 5). Fig. 5 Each Biohit Proline pipettor is fitted with a tip ejector to help eliminate the safety hazards associated with contamination. The tip ejector needs to be pressed firmly downwards to ensure proper tip ejection (Fig.

  • Page 12: Two-In-One Pipettors

    5.3. Two-in-one pipettors The Biohit Proline fixed volume pipettors 5 and 10 µl, and variable volume pipettors 0.5-10 µl can be used with both 10 µl and 300 µl tips. The pipettors are supplied with two ejector collars and are factory fitted with the ejector suitable for 10 µl tips.

  • Page 13: Pipetting Techniques

    Pipetting techniques 1. Make sure that the pipettor, tips and the liquid are at the same temperature. 2. Make sure that the tip is firmly attached to the tip cone. 3. Hold the pipettor vertically when aspirating the liquid and place the tip only a few millimetres into the liquid.

  • Page 14: Reverse Pipetting

    4. The remaining liquid should now be discarded with the tip(s) or delivered back into the container vessel. Storage When not in use it is recommended that your Biohit Proline pipettor is stored in a vertical position. See Installing the pipettor holder (Chapter Testing the performance and recalibration It is recommended to check the performance of your Biohit Proline pipettors regularly (e.g.
  • Page 15 (imprecision) is the scatter of the dispensed volumes around the mean of the dispensed volume. (ISO 8655-1.) Note: Biohit specifications are achieved in strictly controlled conditions (ISO 8655-6). The user should establish own specifications based on the field of use and the accuracy requirements placed on the pipettor (ISO8655-1).

  • Page 16: Recalibration Procedure

    Fig. 10 Maintenance To maintain the best results from your Biohit Proline pipettor each unit should be checked every day for cleanliness. Particular attention should be paid to the tip cone(s). Biohit Proline pipettors have been designed for easy in-house service.

  • Page 17: In-House Maintenance

    5. Remove the tip cone, piston, and spring. Remove the filter if fitted. 6. Place the tip cone, tip ejector, tip ejector collar, piston, O-ring, and spring into a beaker containing Biohit Proline Biocontrol and leave for at least 30 minutes to secure complete decontamination.

  • Page 18: Troubleshooting

    O-ring and piston, too small clean the tip cone (*) Tip ejector Tip cone and/or ejector Clean the tip cone and jammed or collar contaminated the ejector collar (*) moves erratically (*) Clean with Biohit Proline Biocontrol or 75% ethanol.

  • Page 19: Warranty Information

    11. Warranty information Biohit Proline pipettors are covered by warranty for 3 years against defects in materials and workmanship. Should your Proline pipettor fail to function at any time, please contact your local Biohit representative. ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT...
  • Page 20 Specifications Biohit Proline Single Channel Adjustable Volume Pipettors Volume Inaccuracy Imprecision Cat.No. range Volume ± ± 720005 0.1-2.5 µl 2.5 µl 2.50% 2.00% 1.25 µl 3.00% 3.00% 0.25 µI 12.00% 6.00% 720000 0.5-10 µl 10 µl 1.00% 0.80% 5 µl 1.50%...
  • Page 21 3.00% 2.00% 720340 12-ch 50-300 µl 300 µl 0.70% 0.25% 150 µl 1.00% 0.50% 50 µl 1.50% 0.80% Biohit Proline Fixed Volume Pipettors Inaccuracy Imprecision Cat.No. Volume range Volume ± ± 722001 5 µl 5 µl 1.30% 1.20% 722004 10 µl 10 µl...

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