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8.2. Reverse pipetting (Fig. 7)
1. Fit the tip onto the pipette tip cone.
2. Press the operating button all the way to the second stop.
3. Place the tip just under the surface of the liquid (2-3 mm)
and smoothly release the operating button up to the starting
position.
4. Withdraw the tip from the liquid touching against the edge of
the container to remove excess.
5. Press the operating button smoothly to the fi rst stop to deliver
the present volume. Hold the operating button at the fi rst stop.
The liquid that remains in the tip should not be included in the
delivery.
6. Discard the remaining liquid by pressing the operating button
to the second stop.
Fig. 7.
8.3. Repetitive reverse technique
1. Follow the reverse technique steps 1 to 5.
2. Continue pipetting by repeating steps 3 to 5 as long as
needed.
3. Finally discard the remaining liquid by pressing the operating
button to the second stop.
9.
RECOMMENDATIONS FOR GOOD PIPETTING
•
Make sure that the tip is fi rmly attached to the tip cone.
•
Hold the pipette vertically when aspirating the liquid and place
the tip only a few millimeters into the liquid.
•
Always control the operating button slowly and smoothly.
•
Pre-rinse the tip before aspirating the liquid by fi lling and
emptying the tip for three to fi ve times. This is important
especially when pipetting liquids with a viscosity and density
greater than water or liquids with high vapor pressure (e.g.
ethanol).
•
Check that the pipette, tip and liquid are at the same
temperature.
•
When pipetting liquids with temperatures different to the
ambient temperature change the tip after each pipetting. Do
not pre-rinse the tip.
•
To avoid contamination, do not rest the pipette on its side
especially with the tip attached.
•
Change the tip cone fi lter regularly (recommendation after 50
-250 pipetting cycles).
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