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LINE
Käyttöhje
Bruksanvisning
Instruction Manual

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Summary of Contents for Biohit mLINE m10

  • Page 1 ® LINE Käyttöhje Bruksanvisning Instruction Manual...
  • Page 2 LINE ® Käyttöohje ..........1 Bruksanvisning .......... 17 Instruction Manual ........33...
  • Page 3 200, 1000 5000 Varaosat Reservdelar Spare Parts 1 Kärjenpoistajan holkki Spetsavkastarhylsa Tip ejector collar 2 Kärkikartion pidike Spetskonens hållare Tip cone holder Spetskon Tip cone complete 3 Kärkikartio Spetskoncylinder Tip cone cylinder 4 Kärkikartiosylinteri 5 Mäntä Kolv Piston 6 Männän tiiviste Kolvens tätningsställe Piston seal 7 Kalibrointimutterin kansi...
  • Page 4: Table Of Contents

    CONTENTS Product description ................. 34 Biohit tips ..................36 Unpacking ..................36 Personal identification ..............36 Pipettor holder and carousel ............37 5.1. Installing the pipettor holder ............ 37 5.2. Carousel stand ................. 37 Calibration tool (also tube and ID window opener) ...... 37 Operating the pipettor ..............38...
  • Page 5: Product Description

    Product description Your new Biohit LINE is an autoclavable air displacement pipettor. The attractive and ergonomical design of the LINE pipettor together with its very low pipetting forces reduce the risk of repetitive strain injuries (RSI) . The pipettor is designed for both right- and left-handed use.
  • Page 6 Pipettor Description ID window Cap ....... and tag Operating button ....Finger ejector ..support ..Handle Display ... Volume ..Calibration lock ....Colour coding....Tip ejector collar ...... Tip cone....Safe Cone ....filter...
  • Page 7: Biohit Tips

    Biohit tips The full range of Biohit pipettor tips are recommended for use with Biohit LINE pipettors. Biohit standard tips are made of virgin polypropylene. Biohit also offers a full range of filter tips. Biohit standard tips are available as bulk packages, space saving refill systems and autoclavable (121°C, 20 min, 1 atm) trays.
  • Page 8: Pipettor Holder And Carousel

    Pipettor holder and carousel stand For convenience and safety always keep the pipettor vertically on its own holder or carousel stand when not in use. Cat.No. Product 725600 Biohit LINE Carousel Stand 725610 Biohit LINE Pipettor Holder 5.1. Installing the pipettor holder Clean the shelf surface with ethanol.
  • Page 9: Operating The Pipettor

    Do not rotate the operating button outside the volume range of the pipettor (Chapter. 1.) 7.2. Sealing and ejecting tips To ensure the maximum accuracy and precision Biohit tips are recommended to be used with LINE pipettors. Before fitting a tip ensure that the pipettor tip cone is clean.
  • Page 10: Protective Tip Cone Filters And Filter Ejection

    LINE pipettors (> 10 µl) allows the The tip cone design of the Biohit use of Biohit Safe-Cone filters in the tip cones. These removable filters prevent liquids and aerosols from entering the pipettor and, thus, protects the pipettor from contamination and damage.
  • Page 11: Pipetting Techniques

    Pipetting techniques Two basic pipetting techniques, forward and reverse pipetting, are associated with LINE pipettors. Forward pipetting is the most common used pipetting technique. The technique employs the blow- out function ensuring complete delivery of the liquid. Reverse pipetting is recommended for highly viscous, biological or foaming liquids, or very small volumes of liquid.
  • Page 12: Repetitive Reverse Technique

    8.3. Repetitive reverse technique Follow the reverse technique steps 1 to 5. Continue pipetting by repeating steps 3 to 5 as long as needed. Finally discard the remaining liquid by pressing the operating button to the second stop. Recommendations for good pipetting •...
  • Page 13: Maintenance

    LINE pipettors have been designed for easy in-house service. If the pipettor is used daily it is recommended to clean/decontaminate and check the performance of the pipettor every three months. Biohit also provides complete repair and recalibration service for your pipettor including a service report and performance certificate.
  • Page 14: Sterilization Of The Pipettor

    5000) into a beaker containing Biohit Proline‚ Biocontrol and leave for at least 30 minutes. Wipe the piston with Biohit Proline‚ Biocontrol and the lint-free cloth. Rinse the parts with distilled or sterile water. Let the parts dry. Grease the piston and seal according to the instructions given earlier in this chapter.
  • Page 15: Testing The Performance And Recalibration

    11. Testing the performance and recalibration It is recommended to check the performance of your Biohit LINE pipettors regularly (e.g. every 3 months) and always after in-house maintenance. However, the user should establish a regular testing routine for their pipettors with regard to accuracy requirements of the...
  • Page 16 (imprecision) is the scatter of the dispensed volumes around the mean of the dispensed volume. (ISO 8655-1.) Note: Biohit specifications are achieved in strictly controlled conditions (ISO 8655-6). The user should establish own specifications based on the field of use and the accuracy requirements placed on the pipettor (ISO8655-1).
  • Page 17: Recalibration

    The calibration is based on ISO 8655-6: Gravimetric test method for volumetric instruments. The pipettor’s specifications are guaranteed only with genuine Biohit tips. If you find the pipettor to be inaccurate after performance testing, please follow the instructions below: Remove the lid of the calibration nut, located at the backside of the handle, with the aid of the calibration tool (Fig.
  • Page 18: Troubleshooting

    Tip cone holder Tighten the tip incorrectly tightened cone holder Pipettor damaged Return to your Biohit service representative for servicing Pipettor out of Incorrect operation Follow instructions established specifications Unsuitable tip Use original tips...
  • Page 19: Warranty Information

    Should your LINE pipettor fail to function at any time, please contact your local Biohit representative. ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT, MISUSE,...

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