Sterilization Of The Pipette - Biohit PROLINE PLUS Instruction Manual

Pipettes 2000, 5000, 10 000 μl:
Unscrew the tip cone cylinder (3) counterclockwise and remove it.
4. Clean the tip ejector collar, the tip cone holder, the tip cone
(cylinder) and the piston (4) with Biohit Proline Biocontrol,
ethanol (70%), isopropanol (60%) or mild detergent and soft
lint-free cloth.
5. Clean the interior of the tip ejector collar and the tip cone
(cylinder) with a cotton swab. Be careful with the pipettes so
that the seal inside the tip cone will not damage.
6. Rinse the parts with distilled water if needed and let the parts
dry.
7. Pipettes ≤ 100 μl:
Put a thin layer of grease on the piston (4).
Pipettes 250, 500 and 1000 μl:
Put a thin layer of grease around the piston seal (4).
Pipettes 2000, 5000 and 10 000 μl:
Put a thin layer of grease on the piston seal (4) and the interior
of the tip cone cylinder (3).
Note: Avoid excess grease. Use only the grease provided with
the pipette.
Note: Before reassembling check that no lint or particles are on
the surface of the piston.
Decontamination:
For complete decontamination of the lower parts place the tip
ejector collar (1), tip cone holder (2) and tip cone (3) into a beaker
containing Biohit Proline Biocontrol and leave for at least 30
minutes. Wipe the piston with Biohit Proline Biocontrol and the
lint-free cloth. Rinse the parts with distilled or sterile water. Let the
parts dry. Grease the piston and seal according to the instructions
given earlier in this chapter.
Reassembling:
1. Pipettes:
Carefully place the tip cone (3) on the piston and attach it by
screwing the tip cone holder (2) clockwise.
Pipettes 2000, 5000 and 10 000 μl:
Carefully place the tip cone cylinder (3) on the piston and screw
clockwise. Make sure that the tip cone (cylinder) is properly
tightened. Avoid over tightening.
2. Attach the tip ejector collar (1) by screwing it clockwise.
3. Place the new tip cone fi lter on its place.
4. Press the operating button several times to ensure that the
grease has spread evenly.
5. Check the performance of the pipette.
Note: It is always necessary to check the performance of the
pipette after in-house service or maintenance.

10.3. Sterilization of the pipette

The entire Proline
autoclaving at 121°C, (252°F), 1 atm for 20 minutes. Remove the
Plus pipette can be sterilized by steam
®
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