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Related Manuals for Biohit eLine 730020
Summary of Contents for Biohit eLine 730020
- Page 1 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
- Page 2 LINE ® Instruction Manual Bedienungsanleitung Mode d’ emploi Manual Usario Instruzioni d’impiego san Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan...
- Page 3 LINE ® Instruction Manual ........1 Bedienungsanleitung ........35 Mode d’ emploi ..........71 Manual Usario ...........105 Instruzioni d’impiego ........139 san Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan...
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Page 4: Table Of Contents
Reset Button .................6 4.3. Start Button ..................6 4.4. Tip sealing and Electronic tip ejection ........6 4.5. Biohit Safe-Cone Filters ...............7 5. CHARGING OF THE PIPETTE ...............7 5.1. Charging with the Biohit LINE Charging Stand and Carousel 8 5.2. Charging through direct charging system ........9 5.3. -
Page 5: Intended Use
The microprocessor controlled piston movements reduces the possibility of human error and thus also contamination of the pipette interior. The tip cone design of the Biohit LINE pipettes offer the possibility to use replaceable Safe-Cone Filters to help prevent contamination and damage of the pipette. -
Page 6: Biohit
(Fig. 1.) Fig. 1. 3. UNPACKING The Biohit LINE electronic pipettes may be delivered with or without an AC- adaptor, depending from the type of order. However, all Biohit LINE pipette packages contains: • Pipette •... -
Page 7: Pipette Description
Tip Ejector Tip Cone(s) Assembly (excl. 5000) (e5000) Safe-Cone Filter 4.1. Display and controls The control and programming of the Biohit LINE pipette is performed using the display, keyboard and operating buttons. Display: Operating Mode Symbol Mode Range Aliquots/Counter Volume... - Page 8 Mode Ranges and Operating Mode Symbols: PIPET MULTI PROG P = pipetting d = multi-dispensing PROG 1-6 rP = reverse dd = diluting = 6 storage pipetting = diluting MIXING locations = pipetting with mixing MIXING with mixing Sd = sequencial = pipetting with dispensing COUNT...
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Page 9: Reset Button
4.4. Tip sealing and Electronic tip ejection The Biohit pipette tips are recommended for use with Biohit LINE pipettes. The LINE multichannel pipettes are provided with a spring loaded OPTILOAD tip cones for optimal sealing. -
Page 10: Biohit Safe-Cone Filters
(Fig. 4). Fig. 4. The Biohit LINE pipettes should be charged using the original Biohit LINE Charging Stand or Charging Carousel. However, the Biohit LINE also features direct charging by using the Biohit LINE AC-adaptor. -
Page 11: Charging With The Biohit E Line Charging Stand And Carousel
5.1. Charging with the Biohit LINE Charging Stand and Carousel The Biohit LINE Charging Stands and Carousels are bench top charging units. The Charging Stand accommodates one Biohit LINE pipette and the Charging Carousel four Biohit LINE pipettes. The LINE Charging Stands and Carousels... -
Page 12: Charging Through Direct Charging System
5.2. Charging through direct charging system The AC-adaptor supplied with the Biohit LINE pipette or single Charging Stand can be used for direct charging of the Biohit LINE electronic pipette. The socket for the AC-adaptor is placed at the back of the pipette (Fig. 6). -
Page 13: Programming Principle
6. PROGRAMMING PRINCIPLE The Biohit LINE pipettes features 10-11 different operating modes and six different storage locations for frequently used modes with user-selected settings. The operating modes are divided in to three Mode Ranges (PIPET, MULTI, PROG) and each Mode Range includes the following operating... -
Page 14: Programming Map
6.1. Programming map PIPET MULTI PROG PROG 1 PROG 2 PROG 3 + MIXING MIXING PROG 4 MANUAL* PROG 5 COUNT PROG 6 To change all settings To change settings When pre-stored including speed: excluding speed: Prog 1–6 was chosen Start pipetting Repeat until programming... -
Page 15: Speed Adjustment
6.2. Speed adjustment There are 9 speeds available for both dispensing and aspiration. The speed is indicated in the bottom left hand corner of the display. The default speed is 5 for all operating modes. Speed can be adjusted during the programming by completing the setting changes with the select key. -
Page 16: Reverse Pipetting (Rp)
7. Press to confi rm selection and to display the speed out 8. Select the speed out with (1= Min and 9 = Max) 9. Press to confi rm selections 10. Position the tip to aspirate and press the START button 11. -
Page 17: Pipetting With Cycle Counter (P Count)
6. Select the speed in with (1= Min and 9 = Max) 7. Press to confi rm selection and to display the speed out 8. Select the speed out with (1= Min and 9 = Max) 9. Press to confi rm selections 10. -
Page 18: Manual Pipetting (P Manual)
8. Select the speed in with (1= Min and 9 = Max) 9. Press to confi rm selection and to display the speed out 10. Select the speed out with (1= Min and 9 = Max) 11. Press to confi rm selections 12. -
Page 19: Multi Modes
8. Select the speed out with (1= Min and 5 = Max) 9. Press to confi rm selections 10. Position the tip to aspirate and press the START button ▲ Step 10 Note: The aspiration can be interrupted at any time simply by releasing the START button and continued by pressing the START button again. -
Page 20: Multi-Dispensing (D)
8.1. Multi-dispensing (d) The pipette performs repetitive dispensing of a selected volume. The sum of the dispensing aliquots and an automatically selected excess volume is aspirated into the tip. The excess volume is needed to ensure equal operating conditions for each dispensing step. 1. -
Page 21: Diluting (Dd) And Diluting With Mixing (Dd+Mixing)
8.2. Diluting (dd) and diluting with mixing (dd+MIXING) Two different solutions separated with an air gap are aspirated and then dispensed together with an automatic blow-out. The purpose of the air gap is to avoid contamination when aspirating the second volume but it will not prevent the two liquids from mixing in the tip. -
Page 22: Sequenced Dispensing (Sd)
To mix: 1. Position the tip in to the solution, then press and hold the START button The mixing is performed automatically as long as the START button is held down. 2. Release the START button and position the tip to dispense. 3. -
Page 23: Multi-Aspirating (Sa)
11. Select the speed out with (1= Min and 9 = Max) 12. Press to confi rm selections 13. Position the tip to aspirate and press the START button . The display shows PRE OUT and arrow down to indicate the reset function. 14. -
Page 24: Automatic Dispensing (Ad)
12. Position the tips to aspirate and press the START button repeating the action until the cycle is complete. The total volume is displayed. 13. Position the tips to dispense and press START button twice ▲ ▼ Step 12 Step 13 8.5. -
Page 25: Prog Mode
16. To dispense, position the tip, press the START button once and the pipettor dispenses the aliquots at the specifi ed pace. 17. Finally position the tip to discard any remaining excess and press the START button twice ▲ ▼ ▼... -
Page 26: Recall Stored Mode From The Storage Location
START button 10. PIPETTING RECOMMENDATIONS The wide selection of different operating modes makes is possible to perform several different liquid handling tasks with Biohit LINE pipettes. The operating modes P, P+MIXING, P COUNT, dd, dd+MIXING and SA feature an automatic blow-out and others leave an excess liquid in the tip. -
Page 27: Dispensing Without Blow-Out
11. MAINTENANCE To ensure trouble-free operation it is recommended to apply regular maintenance and cleaning to Biohit eLINE electronic pipettes. Note: Remove the battery pack from the pipette prior to servicing! It is recommended always to use gloves when cleaning the pipette. -
Page 28: Cleaning, Decontaminating And Autoclaving The Lower Part Of Pipettes
For complete decontamination place the tip ejector collar (2), tip cone holder (3), tip cone (4), spring (5) and piston (6) into a beaker containing Biohit Proline® Biocontrol and leave for at least 30 minutes (Fig. 9), rinse the parts with distilled water. -
Page 29: Multichannel Pipettes
Note: The autoclavable parts in the 5000 model are the green tip cone (4), the black tip ejector assembly and the piston (6). Note: Avoid excess grease. Use only the grease provided with the pipette. Note: Before reassembling check that no lint or particles are on the surface of the piston. -
Page 30: Battery Replacement
It is also advisable to remove the battery pack from the pipette. This protects the batteries against discharging during the long storage period. 12. PERFORMANCE TESTING It is recommended to check the performance of your Biohit LINE pipettes regularly (e.g. every 3 months) and always after in-house maintenance. However,... - Page 31 Random error is the scatter of the dispensed volumes around the mean of the dispensed volume. (ISO 8655-1.) Note: Biohit specifi cations are achieved in strictly controlled conditions (ISO 8655-6). Users should establish acceptable maximum permissible errors based on the fi...
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Page 32: Recalibration
LINE electronic pipette’s calibration has been factory checked and certifi ed at 22°C using grade 3 distilled water according to ISO 8655. The pipette’s specifi cations are guaranteed only with genuine Biohit tips. Note: Biohit offers an accredited calibration service. Please contact your local Biohit representative for further information. -
Page 33: Trouble Shooting
LINE to complete the execution of the pipetting. The LINE therefore needs to be returned to the nearest Biohit Service Centre or your local Biohit service representative for repair. san Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan... - Page 34 Insuffi cient amount of Grease accordingly grease on a piston and o-ring Pipette out of Instrument damaged Return to your Biohit established service representative specifi cations for servicing Pipette blocked, Liquid has penetrated Clean and grease the...
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Page 35: Warranty Information
MANUAL OR WITH OTHER THAN THE MANUFACTURER’S ORIGINAL TIPS. Each Biohit LINE electronic pipette is tested before shipping by the manufacturer. The Biohit Quality Assurance Procedure guarantees that the Biohit LINE electronic pipette you have purchased is ready for use. Each Biohit LINE electronic pipette is CE/IVD marked, fulfi... - Page 36 Volume Test Number of Cat.No. Channels Range Volume Inaccuracy Imprecision Dispensings 730080 1-ch 50–1000 μl 1000 μl 0.40% 0.15% 500 μl 0.60% 0.20% 100 μl 1.50% 0.50% 50 μl 2.00% 1.00% 1–20 730100 1-ch 100–5000 μl 5000 μl 0.50% 0.15% 2500 μl 0.80% 0.20%...
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Page 37: Recycling Instruction (Weee)
16. RECYCLING INSTRUCTION (WEEE) In compliance with European Directive, WEEE (2002/96EC) on waste and reducting of hazardous substances in electical and electronic equipments, this device must not be recycled as unsorted municipal waste. Instead this device must be collected separately in accordance the local recycling regulations. - Page 38 Patente wurden gewährt oder sind schwebend. Toutes les informations contenues dans cette publication sont à jour au moment de la publication et ont été compilées avec le plus grand soin, Biohit n’est pas responsable des éventuelles erreurs ou omissions et se réserve le droit d’apporter des modifications sans préavis.
- Page 39 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
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