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CONTENTS
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Table of Contents
Related Manuals for Biohit eLINE 73002X
Summary of Contents for Biohit eLINE 73002X
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Page 1: Table Of Contents
Display and controls 3.2. Reset button 3.3. Start button 3.4. Tip sealing and Electronic tip ejection 3.5. Biohit Safe-Cone filters Charging of the pipettor 4.1. Charging with the LINE Charging Stand and Carousel 4.2. Charging through the direct charging system 4.3. -
Page 2: Biohit
The microprocessor controlled piston movements reduces the possibility of human error and thus also contamination of the pipettor interior. The tip cone design of the Biohit LINE pipettors offer the possibility to use replaceable Safe-Cone Filters to help prevent contamination and damage of the pipettor. -
Page 3: Biohit Tips
PLEASE REMOVE THE PLASTIC PROTECTOR UNDER THE BATTERY COVER. Note: It is recommended to recharge the pipettor for at least two hours before initial use. The pipettor may only be charged with original Biohit LINE charging stand, charging carousel or Biohit LINE AC-adaptor. -
Page 4: Pipettor Description
Tip Ejector Tip Cone(s) (excl. 5000) Assembly (e5000) Safe-Cone Filter 3.1. Display and controls The control and programming of the Biohit LINE pipettor is performed using the display, keyboard and operating buttons. Display: Operating Mode Symbol Mode Range Aliquots/Counter Volume... - Page 5 Mode Ranges and Operating Mode Symbols: PIPET MULTI PROG P = pipetting d = multi-dispensing PROG 1-6 = 6 storage rP = reverse dd = diluting locations pipetting = diluting MIXING = pipetting with mixing MIXING with mixing Sd = sequencial = pipetting with dispensing COUNT...
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Page 6: Reset Button
3.4. Tip sealing and Electronic tip ejection The Biohit pipettor tips are recommended for use with Biohit LINE pipettors. The... -
Page 7: Biohit Safe-Cone Filters
3.5. Biohit Safe-Cone Filters The tip cone design of the Biohit LINE pipettors (>10 µl) allows the use of Biohit Safe-Cone Filters in the tip cones. These removable filters prevent liquids and liquid vapours from entering the pipettor and thus... -
Page 8: Charging Of The Pipettor
Biohit LINE Charging Stand or Charging Carousel. However, the Biohit LINE also features direct charging by using the Biohit LINE AC-adaptor. Fig. 4. Note: If the battery is fully discharged, the display will be empty and there will be no response from the operating buttons. -
Page 9: Charging Through The Direct Charging System
Charging unit. This ensures that the battery is kept charged and the pipettor is ready for use. 4.2. Charging through direct charging system The AC-adaptor supplied with the Biohit LINE pipettor or Charging Stand can be used for direct charging of the Biohit LINE electronic pipettor. -
Page 10: Electrical Specifications
- Input voltages and main plug according to local requirements - Output voltage 9 VDC, 1200 mA Programming principle The Biohit LINE pipettors features 10-11 different operating modes and six different storage locations for frequently used modes with user-selected settings. The operating modes are divided in to three Mode Ranges (PIPET,... -
Page 11: Programming Map
5.1. Programming map PIPET MULTI PROG PROG 1 PROG 2 PROG 3 + MIXING MIXING PROG 4 MANUAL* PROG 5 COUNT PROG 6 To change all settings To change settings When pre-stored including speed: excluding speed: Prog 1–6 was chosen Start pipetting Repeat until programming... -
Page 12: Speed Adjustment
5.2. Speed adjustment There are 9 speeds available for both dispensing and aspiration. The speed is indicated in the bottom left hand corner of the display. The default speed is 5 for all operating modes. Speed can be adjusted during the programming by completing the setting changes with the select key. - Page 13 Select the speed in with (1= Min and 9 = Max) Press to confirm selection and to display the speed out Select the speed out with (1= Min and 9 = Max) Press to confirm selections 10. Position the tip to aspirate and press the START button 11.
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Page 14: Reverse Pipetting (Rp)
6.2. Reverse pipetting (rP) A selected volume plus and excess is aspirated into the tip. After dispensing the selected volume the excess volume remains in the tip and is discarded. Press to display PIPET Scroll with until the symbol rP is displayed. Confirm by pressing Select the desired pipetting volume by using Note: When holding down... -
Page 15: Pipetting With Cycle Counter ( Pcount )
6.3. Pipetting with cycle counter COUNT The eLINE performs pipetting with blow-out and the cycle counter function. The cycle counter counts up to 99 pipettings and then returns back to 0. It is possible to choose the cycle number to begin with. It is also possible to change the cycle number or reset the counter during the pipetting by using the arrow keys. -
Page 16: Manual Pipetting ( Pmanual )
6.4. Manual pipetting MANUAL (Not available in multichannel models) In the manual pipetting mode the aspiration and dispensing are controlled manually. The aspiration or dispensing continues as long as the START button is held down (within the selected volume range). The aspiration/dispensing direction can be changed during pipetting by using the arrow key. -
Page 17: Multi Modes
11. Press the to change the aspiraton/dispensing direction. 12. Position the tip to dispense. Press the START button 13. When the tip is empty (volume reaches 0 µl), within one second the display will show E. Position the tip to empty the tip with blow-out and press the START button twice. - Page 18 Press to confirm the selection and to display the aliquots Select the desired number of aliquots with Press to confirm selection and to display the speed in or press and continue from Step 12. Select the speed in with (1= Min and 9 = Max) Press to confirm selection and to display the speed out 10.
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Page 19: Diluting (Dd) And Diluting With Mixing
7.2. Diluting (dd) and diluting with mixing (dd+ MIXING Two different solutions separated with an air gap are aspirated and then dispensed together with an automatic blow-out. The purpose of the air gap is to avoid contamination when aspirating the second volume but it will not prevent the two liquids from mixing in the tip. -
Page 20: Sequenced Dispensing (Sd)
To mix: Position the tip in to the solution, then press and hold the START button The mixing is performed automatically as long as the START button is held down. Release the START button and position the tip to dispense. Empty the tip by pressing the START button twice Step 1 Step 3... -
Page 21: Multi-Aspirating (Sa)
Select the speed in with (1= Min and 9 = Max) 10. Press to confirm selection and to display the speed out 11. Select the speed out with (1= Min and 9 = Max) Step 13 12. Press to confirm selections 13. -
Page 22: Automatic Dispensing (Ad)
Press to confirm the selection Select the desired number of aspirations with Press to confirm selection and to display the speed in or press and continue from Step 12. Select the speed in with (1= Min and 9 = Max) Press to confirm selection and to display the speed out 10. - Page 23 Press to confirm the selection and to display the aliquots Select the desired number of aliquots with Press to confirm selection Select the desired dispensing pace with (0.1 - 9.9 seconds) Press to confirm the selection and to display the speed in or press and continue from Step 14.
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Page 24: Prog Modes
Note: Furthermore it is possible to continue to aspirate and dispense the same volume without the empty function. To continue, press the START button immediately after E is displayed and within one second the direction arrow will change. Keeping the START button depressed, place the tip into the liquid again and then release the START button to aspirate the next sample. -
Page 25: Pipetting Recommendations
4. The pipettor is ready to perform according to the stored mode. Position the tip to aspirate and press START button Pipetting recommendations The wide selection of different operating modes makes is possible to perform several different liquid handling tasks with Biohit LINE pipettors. The operating modes P, P , dd, dd+... -
Page 26: Maintenance
80%). 10. Maintenance To ensure trouble-free operation it is recommended to apply regular maintenance and cleaning to Biohit LINE electronic pipettors. Note: Remove the battery pack from the pipettor prior to servicing! It is recommended always to use gloves when cleaning the pipettor. - Page 27 For complete decontamination place the tip ejector collar (2), tip cone holder (3), tip cone (4), spring (5) and piston (6) into a beaker containing Biohit Proline ® Biocontrol and leave for at least 30 minutes (Fig. 9), rinse the parts with distilled water.
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Page 28: Battery Replacement
Note: It is always necessary to check the performance of the pipettor after in- house service or maintenance (see paragraph 11). 10.3. Battery replacement The design of the Biohit LINE pipettors enables fast and easy battery replacement. The battery replacement pack includes 4 NiMH batteries fixed to the battery cover. -
Page 29: Storage
It is also advisable to remove the battery pack from the pipettor. This protects the batteries against discharging during the long storage period. 11. Performance Testing It is recommended to check the performance of your Biohit LINE pipettors regularly (e.g. every 3 months) and always after in-house maintenance. - Page 30 Random error is the scatter of the dispensed volumes around the mean of the dispensed volume. (ISO 8655-1.) Note: Biohit specifications are achieved in strictly controlled conditions (ISO 8655-6). Users should establish acceptable maximum permissible errors based on the field of use and the accuracy requirements placed on the pipettor (ISO 8655-1).
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Page 31: Recalibration
C using grade 3 distilled water according to ISO 8655. The pipettor’s specifications are guaranteed only with genuine Biohit tips. Note: Biohit offers an accredited calibration service. Please contact your local Biohit representative for further information. The length of the piston stroke is electronically monitored and the pipettor does not normally need recalibration. -
Page 32: Trouble-Shooting
Note: Repeated occurrence of error messages indicates an internal error causing failure of the LINE to complete the execution of the pipetting. The LINE therefore needs to be returned to the nearest Biohit Service Centre or your local Biohit service representative for repair. - Page 33 Insufficient amount of Grease accordingly grease on a piston and o-ring Pipettor out of Instrument damaged Return to your established specifications Biohit service representative for servicing Pipettor blocked, aspirated Liquid has penetrated Clean and grease volume too small tip cone and dried...
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Page 34: Warranty Information
Should your LINE pipettor fail to function according to specifications at any time, please contact your local Biohit representative immediately. ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT, MISUSE,... - Page 35 Volume Test Number of Cat.No. Channels Range Volume Inaccuracy Imprecision Dispensings 730320 8-ch 0.2–10 µl 10 µl 0.90% 0.50% 5 µl 1.50% 0.80% 1 µl 4.00% 3.00% 1–50 730340 8-ch 5–120 µl 120 µl 0.50% 0.20% 60 µl 0.70% 0.30% 12 µl 2.00% 1.50%...
- Page 36 Notes...
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