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Proline
Käyttöohje
Bruksanvisning
Instruction Manual

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Summary of Contents for Biohit Proline 710520

  • Page 1 ® Proline Käyttöohje Bruksanvisning Instruction Manual...
  • Page 2 Proline ® Käyttöohje.............1 Bruksanvisning ...........23 Instruction Manual ........45 Specifications ..........67...
  • Page 5: Table Of Contents

    CONTENTS 1. INTENDED USE ..................46 2. YOUR NEW BIOHIT PROLINE ELECTRONIC PIPETTE ....46 2.1. Biohit Proline single-channel electronic pipettes ....46 2.2. Biohit Proline multichannel electronic pipettes ..... 47 2.3. Biohit tips ................47 3. UNPACKING & PREPARING THE PIPETTE FOR USE ....48 3.1.
  • Page 6: Intended Use

    IVD-product as an IVD-accessory and as a stand alone laboratory equipment. 2. YOUR NEW BIOHIT PROLINE ELECTRONIC PIPETTE Your new Biohit Proline Electronic pipette has been designed for maximum performance and accuracy with ergonomic and user-friendly operation. The microprocessor controlled system reduces the possibility for human error and instrument contamination by controlling all piston movements.
  • Page 7: Biohit Proline Multichannel Electronic Pipettes

    Biohit pipettes. These detachable, disposable tips are made of natural colour polypropylene. Biohit also offers a full range of fi lter tips. Biohit standard tips are available as bulk packages, space saving refi ll systems and autoclavable (121°C, 20 min, 1 atm) trays.
  • Page 8: Unpacking & Preparing The Pipette For Use

    Make sure that all items are included and that no damage has occured during shipment. Note: The pipette can only be charged with the Biohit Proline charging stand or carousel (Fig. 2 and 3). 3.1. Biohit Proline Charging Stands and Carousels Cat.
  • Page 9: Charging Of The Pipette

    3.2. Charging of the Pipette The charging stand or carousel operates via a contactless system which is free from maintenance and sensitive metal contacts. The pipette features an ON/OFF switch located at the top of the grip of the pipette (Fig. 4). This switch protects the battery from discharging during long storage periods.
  • Page 10: Pipette Materials

    4. PIPETTE MATERIALS Accu cover (ASA/PC) Display Keyboard (glass) (silicon) Finger Start support button (back) (POM) (ASA/PC) Handle Accu cover (ASA/PC) Tip ejector (ASA/PC) level (POM) Display Keyboard (glass) (silicon) Tip ejector collar (PP) Finger support Start (back) button (ABS) (POM) Tip cone Tip ejector...
  • Page 11: Pipette Description

    5. PIPETTE DESCRIPTION The control and programming of the Proline electronic pipette are done using the keyboard and display shown in detail below. Battery Compartment 5.1. Controls Power Switch Changes speeds Moves to Next Mode Increases volume or speed Decreases volume or speed Confi...
  • Page 12: Sealing And Ejecting Tips

    fi lter as an option. The fi lter prevents liquids and liquid vapours from entering the pipette. The fi lter does not affect the calibration of the pipette. Biohit fi lter forceps should be used to avoid touching the Fig. 8 dirty fi...
  • Page 13: Programming The Pipettor

    6. PROGRAMMING THE PIPETTOR Programming is done using the six-button keyboard and the LC-display. There are four to six different operating modes, offering special functions and varying speeds for your selection. 6.1. Mode Selection and Mode Recall 1. Press repeatedly to view the available modes of the pipette. 2.
  • Page 14: Pipetting Mode (P)

    6.3. Pipetting Mode (P) (all models) The pipette performs ordinary pipetting with blow-out. 1. Press to display 2. Press to confi r the mode change. 3. Select the desired pipetting volume by using to increase, and to decrease. Note: when holding down , the volume display starts to scroll faster.
  • Page 15: Multi-Dispensing Mode (D)

    6.5. Multi-Dispensing Mode (d) (all models) The pipette performs repetitive dispensings of a selected volume. During this operation, the desires volume plus and automatically selected excess volume is aspirated into the tip. The excess volume is needed to ensure equal operating conditions for each dispensing. 1.
  • Page 16: Diluting Mode (Dd)

    6.6. Diluting Mode (dd) (all models, except 710800 and 710810) Two different solutions separated by an air gap are aspirated and then dispensed together with automatic blow-out. The purpose of the air gap is to avoid contamination when aspirating the second volume but it will not prevent the two liquids from mixing in the tip.
  • Page 17 For Pipetting: 1. Press to increase, and to decrease. 2. Press to confi rm selection. 3. Position the tip to aspirate and press START button. 4. Position to dispense and press the START button. For Diluting: 1. Select the desired diluent volume (volume 1) by using 2.
  • Page 18: Sequenced Dispensing Mode (Sd)

    6.8. Sequenced Dispensing Mode (sd) (models 710500, 710040 and 710800 only) A series of different volumes can be delivered in any desired order. 1. Press to display 2. Confi rm the mode change by pressing 3. Select the fi rst desired dispense volume onto the display by using 4.
  • Page 19: Pipetting Recommendations

    4. Confi rm selection by pressing 5. Press to select the number of aspirations desired. 6. Confi rm your selection by pressing 7. Position the tip to aspirate and press the START button, repeating the action until the cycle is complete. The total volume is displayed. 8.
  • Page 20: Dispensing Without Blow-Out

    • Change the fi lter on the tip cone regularly (after 50-250 pipettings). 8. STORAGE When not in use it is recommended that the pipettor is stored in the ON position in the Biohit Proline charging stand or carousel.
  • Page 21: Calibration

    Each Biohit Proline Electronic pipette’s calibration has been factory checked and certifi ed at 22°C using distilled water according to ISO 8655- 6. Pipette specifi cations are guaranteed only with genuine Biohit tips. Note: Biohit offers full calibration and accredited calibration service. Please contact your local Biohit representative.
  • Page 22: Testing The Performance

    Biohit Proline Electronic pipettes require regular cleaning to ensure trouble- free operation. To clean and decontaminate the pipette lightly spray the outside of the pipette with Biohit Proline Biocontrol (cat.no. 724004, 5l) or ethanol. Wipe dry with a soft cloth. DO NOT AUTOCLAVE. Change the tip cone fi...
  • Page 23: Cleaning The Tip Cone

    Note: For complete decontamination place the tip cone, tip ejector collar, piston, O-ring and spring into a beaker containing Biohit Proline Biocontrol and leave for at least 30 minutes, rinse the parts with distilled water, then dry preferably with warm air.
  • Page 24: Trouble-Shooting

    6. Dispose of battery appropriately. 11. TROUBLE-SHOOTING Biohit Proline pipettes have a built-in monitoring program to control the performance of each pipetting action. If the error message Er1 appears on the display, this means the pipette has been unable to perform the attempted action properly.
  • Page 25 Clean and grease aspirated volume cone and dried piston and tip cone too small Tip ejector jammed Tip cone contaminated Remove ejector or moves erratically collar, clean with Biohit Proline Biocontrol or ethanol Continuous error Instrumentet skadat Send for service message...
  • Page 26: Warranty Information

    Each Biohit Proline Electronic pipette is tested before shipping by the manufacturer. The Quality Assurance Procedure is your guarantee that the Biohit Proline Electronic pipette you have purchased is ready for use. All Biohit Proline electronic pipettes and charging stands are CE-marked, fulfi...
  • Page 27 SPECIFICATIONS Note: The manufacturer’s specifications below should be used as guidelines when establishing your own performance specifi cations in accordance with ISO 8655. Cat. No. Volume Test Inaccu- Impre- Number of Range Volume racy cision Dispensings 710520 1-ch. 0.2-10 μl 10 μl 0.90% 0.50%...
  • Page 28 Cat. No. Volume Test Inaccu- Impre- Number of Range Volume racy cision Dispensings 710410 4-ch. 5-100 μl 100 μl 0.50% 0.20% 2 - 20 50 μl 0.80% 0.30% 10 μl 1.50% 1.50% 5 μl 4.00% 2.50% 710420 4-ch. 25-250 μl 250 μl 0.40% 0.15%...
  • Page 29 Proline ® är ett registrerat varumärke. Due to Biohit’s continuing R&D effort, specifications may change without prior notice. Biohit Proline innovations are covered by multiple patents and patents pending. Biohit Proline ® is a registered trademark of Biohit Plc. 400145.05 • 10/2010 • © Biohit Oyj...

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