Testing The Performance And Recalibration; Testing The Performance - Biohit PROLINE PLUS Instruction Manual

Fig. 8.
360°
(Fig. 8). Put the pipette into the sterilization bag and place it into
the autoclave. After autoclaving the pipette must be cooled down
and left to dry overnight before use. Screw the lower part of the
multichannel pipettes by holding the connecting collar and turning the
lower part 360° clockwise. Make sure that the lower part is properly
tightened. It is recommended to check the performance of the pipette
after every autoclaving. It is also recommended to grease the piston/
seal of the pipette after every 10 autoclaving.

11. TESTING THE PERFORMANCE AND RECALIBRATION

It is recommended to check the performance of your Biohit
Proline Plus pipettes regularly (e.g. every 3 months) and always
®
after in-house maintenance. However, the user should establish a
regular testing routine for their pipettes with regard to accuracy
requirements of the application, frequency of use, number of op-
erators using the pipette, nature of the liquid dispensed and the
acceptable maximum permissible errors established by the user.
(ISO 8655-1.)

11.1. Testing the performance

Performance testing should take place in a draught-free room at
15 - 30°C, constant to +/- 0.5°C and humidity above 50%. The
pipette, tips and the test water should have stood in the test room
a suffi cient time (at least 2 hours) to reach equilibrium with the
room conditions. Use distilled or deionised water (grade 3, ISO
3696). Use an analytical balance with a readability of 0.01 mgs.
(ISO 8655-6.)
Weighing
1. Adjust the desired test volume V
2. Carefully fi t the tip onto the tip cone.
3. Fill the tip with test water and expel to waste fi ve times to reach
a humidity equilibrium in the dead air volume.
4. Replace the tip. Pre-wet the tip by fi lling it once with test water
and expel to waste.
5. Aspirate the test water, immersing the tip only 2-3 mm below
the surface of the water. Keep the pipette vertical.
6. Withdraw the pipette vertically and touch the tip against the
inside wall of the test water container.
7. Pipette the water into the weighing vessel, touching the tip
against the inside wall of the vessel just above the liquid
surface at an angle of 30° to 45°. Withdraw the pipette by
drawing the tip 8-10 mm along the inner wall of the weighing
vessel.
10
Connecting
collar
tip cone fi lter (if fi tted, Chapter
7.3). The single channel
pipettes can be autoclaved
without special preparations.
Unscrew the lower part of
the multichannel pi-pettors
by holding the connecting
collar and turning the lower
part 360° counterclockwise
.
s