PerkinElmer Raman Flex 400 Series Getting Started Manual
  1. Manuals
  2. Brands
  3. PerkinElmer Manuals
  4. Laboratory Equipment
  5. Raman Flex 400 Series
  6. Getting started manual
PerkinElmer Raman Flex 400 Series Getting Started Manual

PerkinElmer Raman Flex 400 Series Getting Started Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
MOLECULAR SPECTROSCOPY
RamanFlex 400 Series
Getting Started Guide

Advertisement

Table of Contents
loading

Related Manuals for PerkinElmer Raman Flex 400 Series

Summary of Contents for PerkinElmer Raman Flex 400 Series

  • Page 1 MOLECULAR SPECTROSCOPY RamanFlex 400 Series Getting Started Guide...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    Table of Contents Introduction ....................5 About This Manual .................... 6 Conventions Used in this Manual ................ 7 Notes, Cautions and Warnings ..............7 Safety Practices ..................11 Overview ......................12 General Operating Conditions................13 Fire Safety .......................14 Electrical Safety ....................15 Electrical Protection ...................15 Power Supplies ..................15 Power Cords (Between PSU and Power Outlet Socket) .........16 Fuses ......................16...
  • Page 4 Cleaning the Spectrometer and Power Supply Units ........68 Cleaning the Air Filters ................69 Servicing the Spectrometer ................ 70 Service of the Fiber Optic Probe ..............70 Troubleshooting ....................71 Hardware ....................71 Software....................71 Decontamination ..................... 72 WEEE Instructions for PerkinElmer Products ............73...

  • Page 5: Introduction

    Introduction...

  • Page 6: About This Manual

    6 . RamanFlex 400 Series Getting Started Guide About This Manual This Getting Started Guide contains all of the information that you will need to setup your RamanFlex 400 Series Raman Spectrometer and start collecting spectra. The Getting Started Guide is divided into the following chapters: Introduction This chapter contains an overview of the manual, and the conventions and warnings used.

  • Page 7: Conventions Used In This Manual

    ALT+F. All eight-digit numbers are PerkinElmer part numbers unless stated otherwise. The term RamanFlex 400 Series means the RamanFlex 400 and the RamanFlex 400F Raman Spectrometers.
  • Page 8 8 . RamanFlex 400 Series Getting Started Guide We use the term CAUTION to inform you about situations that could CAUTION result in serious damage to the instrument or other equipment. Details about these circumstances are in a box like this one. Caution (Achtung) Bedeutet, daß...
  • Page 9 Introduction . 9 We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons. Details about these circumstances are in a box like this one. WARNING Warning (Warnung) Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer Verletzung des Benutzers kommen kann.
  • Page 10 10 . RamanFlex 400 Series Getting Started Guide...

  • Page 11: Safety Practices

    Safety Practices...

  • Page 12: Overview

    All information provided herein is for reference only. All information is believed to be correct and complete. PerkinElmer shall not be liable for errors contained herein nor damages concerned with the use of this document. PerkinElmer does not accept liability for the interpretation of information resulting, either directly or indirectly, from our spectrometers or associated software interfaces.

  • Page 13: General Operating Conditions

    WARNING The RamanFlex 400 Series spectrometers have been designed and tested in accordance with PerkinElmer specifications and in accordance with the safety requirements of the International Electrotechnical Commission (IEC). The RamanFlex 400 Series conforms to IEC 61010-1 (Safety Requirements for electrical equipment for measurement, control and laboratory use) as it applies to IEC Class 1 (earthed) appliances and therefore meets the requirements of EC directive 2006/95/EC.

  • Page 14: Fire Safety

    14 . RamanFlex 400 Series Getting Started Guide Fire Safety Ensure that there is at least 15 cm (6”) between any cooling vents and adjacent • equipment or walls. Ensure that the power supply unit (PSU) vents or fan do not become obstructed. •...

  • Page 15: Electrical Safety

    NOTE: If you have a deep depletion CCD installed in your RamanFlex, the instrument will be powered by two external PSUs. Under no circumstances should you attempt to disassemble the PSU and carry out repairs. If the PSU has a fault, please contact your PerkinElmer Service Engineer. The entire unit must be replaced. WARNING...

  • Page 16: Power Cords (Between Psu And Power Outlet Socket)

    16 . RamanFlex 400 Series Getting Started Guide Connect the PSU to a three-wire (live, neutral and ground/earth) socket. The ground must be a non-current carrying wire that is connected to the earth ground at the main distribution box. The power point (socket) for the instrument PSU should be easily accessible for when the instrument is serviced.

  • Page 17: Emc Compliance

    Safety Practices . 17 EMC Compliance EC directive The RamanFlex 400 Series instruments have been designed and tested to meet the requirements of the EC directive 2004/108/EC. The RamanFlex 400 Series complies with the EMC standard EN61326 (EMC standard for electrical equipment for measurement, control and laboratory use), and EN55011 (ISM) Class A (RF emissions).

  • Page 18: Laser Safety

    If the protective covers or service panels on the instrument appear to be damaged, turn the instrument off, secure it against any unauthorized WARNING operation and contact PerkinElmer for advice before using the instrument again. The RamanFlex 400 Series are classified as Class 3B. The Fiber Optic Probe provides a focused beam of laser radiation at 785 nm.

  • Page 19: Class 3B Safety Precautions

    Safety Practices . 19 Class 3B Safety Precautions You should follow Class 3B laser safety precautions such as the use of door interlocks and the wearing of laser safety goggles. For information on these precautions refer to an appropriately trained laser safety officer within your own facility. Laser safety is very important.
  • Page 20 Use protective laser safety goggles, with an OD (optical density) of at least 3 at 785 nm, • as a precaution against accidental exposure to the direct or reflected laser light. Suitable goggles are available from PerkinElmer (part number L1323518).

  • Page 21: Triggered Fiber Optic Probe Safety

    Do not use the TFOP if it shows any sign of damage. • • If repair or servicing is needed, call your PerkinElmer Service Representative for advice. Raman Triggered Fiber Optic Probe For further safety details, see the leaflet (part number L1321940).

  • Page 22: Internal Interlock System

    7.5 (± 10%) 27.3 NOTE: Other focal lengths are available; contact PerkinElmer for details. In particular, the use of an ultra-long working distance lens adaptor (part number L1320071) provides a working distance of 25 mm. Where different focal lengths are used, the NOHD should be calculated accordingly.

  • Page 23: Warning Labels

    Safety Practices . 23 Warning Labels When this label is attached to an instrument it means refer to the manual to find out the nature of the potential hazard and any actions which have to be taken. Labels on the Exterior of the RamanFlex 400/400F The following labels are displayed on the exterior of the instrument: LASER RADIATION LASER RADIATION...
  • Page 24 24 . RamanFlex 400 Series Getting Started Guide...

  • Page 25: Raman History, Theory And Instrumentation

    Raman History, Theory and Instrumentation...

  • Page 26: The History Of Raman Spectroscopy

    26 . RamanFlex 400 Series Getting Started Guide The History of Raman Spectroscopy Inelastic light scattering was first predicted in 1923 by A.G. Smekal in Germany. Research carried out by A. H. Compton discovered inelastic scattering of X-rays, which led the Indian scientist C.

  • Page 27: Raman Theory

    Raman History, Theory and Instrumentation . 27 Raman Theory When photons from a monochromatic light source impinge on a sample, a small fraction are scattered in all directions. Some of the scattered photons are of the same energy as those of the incident beam, and hence the same frequency, giving rise to Rayleigh scattering (Figure 4).
  • Page 28 28 . RamanFlex 400 Series Getting Started Guide The change between the incident radiation and the Stokes/Anti-Stokes radiation represents a change in the vibrational and/or rotational energy of the molecule. The frequency shifts from the frequency of the incident beam are displayed on the x-axis of a Raman spectrum, generally in relative wavenumbers (otherwise known as Raman Shift) and the intensity of the bands are displayed in arbitrary units on the y-axis.

  • Page 29: The Ramanflex 400 Series

    Raman History, Theory and Instrumentation . 29 The RamanFlex 400 Series The RamanFlex 400 Series are fiber-optic Raman spectrometers with laser excitation wavelength at 785 nm. Figure 7 The RamanFlex 400 Series The instrument (Figure 7) is composed of a laser, mirrors, fiber optics, filters, lenses, spectrograph and a CCD detector.

  • Page 30: Spectrograph Detector Systems

    Lens #1 Figure 9 Schematic of the Echelle Spectrograph PerkinElmer’s Echelle detector is shipped in the RamanFlex 400 Series. The system contains two components; the Echelle spectrograph and a two-dimensional CCD detector. The system works by dispersing the light in two dimensions by using two gratings (Figure 9). The first grating disperses the light horizontally onto the second grating, where the light is further dispersed vertically onto the CCD detector.
  • Page 31 No light is lost, all the light goes onto the detector, and all the strips are readout simultaneously. The concept of an Echelle detector is not unique to PerkinElmer. Echelle detectors are commonly used in applications where high resolution...
  • Page 32 32 . RamanFlex 400 Series Getting Started Guide...

  • Page 33: Preparing Your Facility

    Preparing your Facility...

  • Page 34: Specifications

    Power Supply 100–230 V AC ±10%, 50–60 Hz If the electricity supply does not conform to these specifications, please consult PerkinElmer prior to installation. If the supply of power is erratic please use an uninterruptible power supply (UPS). Incorrect shutdown, power fluctuations or brown-outs may damage the...

  • Page 35: Finding A Suitable Location For The Instrument

    Preparing your Facility . 35 Finding a Suitable Location for the Instrument The following conditions should be observed: The environment must be suitable for a Class 3B laser system. • • There must be a minimum of 3 power points (1 for the spectrometer, 2 for the PC). If your system has a deep depletion CCD you will need 2 additional power points (1 for the CCD PSU and 1 for the recirculating water cooler).

  • Page 36: Instrument Dimensions

    36 . RamanFlex 400 Series Getting Started Guide Instrument Dimensions The RamanFlex 400 spectrometer weighs approximately 20 kg. Laboratory benching must be strong enough to support the combined weight of the instrument and any ancillary equipment without warping or wobbling. The bench must be at least 650 mm (26”) in depth. 161 mm 600 mm 530 mm...

  • Page 37: Installing Your Ramanflex 400 Series

    Installing your RamanFlex 400 Series...

  • Page 38: Unpacking The Instrument

    When unpacked, check that you have the following parts and check for any physical damage. If there is any damage, do not continue with the installation. Contact your PerkinElmer Service Representative immediately. Part Number...

  • Page 39: Lifting The Instrument

    Installing your RamanFlex 400 Series . 39 Lifting the Instrument The RamanFlex 400 Series spectrometer module weighs 20 kg. To avoid injury or damage to the instrument, please use proper lifting techniques. Always hold the instrument under the base when you lift it. WARNING...

  • Page 40: Attaching The External Interlock Override

    (part number L1320264) supplied with the instrument. If your Laser Safety Officer determines that an interlock is required on the door to the room, you will need to purchase the Raman Laser Safety Kit (part number L1320262). For more information contact your PerkinElmer representative.

  • Page 41: Installing The Instrument In Spectrum Software

    You must not connect the instrument USB cable to the PC before the Spectrum software has been installed. NOTE: A PerkinElmer Service Engineer will install the Spectrum software on the PC. However, details of the software installation can be found in the Spectrum Administrator’s Guide...

  • Page 42: Switching On The Instrument

    The RamanFlex is supplied with two keys. One should be placed in the instrument and the other should be stored in a safe place. Replacement keys (part number L1323503) can be obtained from PerkinElmer. A laser safety officer or lab manager can control use of the instrument with these keys.

  • Page 43: Moving The Instrument

    Installing your RamanFlex 400 Series . 43 Moving the Instrument If you need to ship your instrument you must use the original supplied packing materials to prevent damage to the system. When the instrument has reached its destination and has been reassembled, you should Calibration perform the appropriate calibration/verification procedures as described in the and Performance Verification SOPs...
  • Page 44 44 . RamanFlex 400 Series Getting Started Guide...

  • Page 45: Using The Ramanflex 400 Series

    Using the RamanFlex 400 Series...

  • Page 46: Spectrometer Front Panel

    46 . RamanFlex 400 Series Getting Started Guide Spectrometer Front Panel The front panel of the spectrometer module contains 3 LED indicators and 1 key switch (Figure 12). Figure 12 Spectrometer module front panel Description Power If the Power LED is illuminated, this indicates that the instrument is receiving power.

  • Page 47: Fiber Optic Probe

    DO NOT ATTEMPT TO USE THE PROBE BODY IN SUCH AN ENVIRONMENT WITHOUT THE USE OF AN IMMERSION SLEEVE (part number L1320003). If required, contact PerkinElmer for further details. Attaching & Removing the Fiber Optic Probe...
  • Page 48 48 . RamanFlex 400 Series Getting Started Guide 4. Remove the two retained screw-in caps from the coupler ports at the bottom left of the panel. If you have a RamanFlex 400F and wish to connect a second fiber optic probe, then remove the retained screw-in caps from the two couplers below the LED FIBER 2 connector.

  • Page 49: Acquiring Spectra With The Fiber Optic Probe

    Ensure an immersion sleeve is in place before immersing your FOP, see Figure 14. Please contact PerkinElmer if you WARNING have any questions in relation to sampling using the FOP.
  • Page 50 50 . RamanFlex 400 Series Getting Started Guide Fiber optic probe Immersion sleeve Figure 14 Fiber optic probe with the immersion sleeve detached Non Immersion When using 785 nm lasers, many types of glass exhibit fluorescent bands between −1 1400–1600 cm .

  • Page 51: General Sops

    PSU and check all of the connections, any power socket switches and then switch the PSU on again. 7. From the Start menu select All Programs; the PerkinElmer Applications group; the Spectrum sub-group and then the Spectrum application.

  • Page 52: Sop-2: Turning The Instrument Off

    52 . RamanFlex 400 Series Getting Started Guide The Spectrum start-up screen is displayed, followed by a dialog that may require your login details: 8. If required, enter your User name and Password, and then click OK. 9. Select the Instrument you want to use and click Connect. If you want to work with data that has been collected previously, without connecting to an instrument, select Work Offline.

  • Page 53: Sop-3: Setting-Up Experimental Parameters And Collecting Data

    Using the RamanFlex 400 Series . 53 Troubleshooting NOTE: If Spectrum software stops responding, refer to starting on page 71 for details of how to shut down the instrument and restart the software. SOP-3: Setting-Up Experimental Parameters and Collecting Data This SOP is designed to act as a quick guide to help you start collecting data.
  • Page 54 54 . RamanFlex 400 Series Getting Started Guide 11. If you select Save after each measurement, your spectra will automatically be saved using the Sample ID. 12. When you have finished defining your experimental parameters, position your sample as SOP-5: Sample Positioning when using a Fiber Optic Probe described in 13.

  • Page 55: Fiber Optic Probe Sops

    Using the RamanFlex 400 Series . 55 Fiber Optic Probe SOPs NOTE: If you have a RamanFlex 400F with two fiber optic probes, ensure that the probe not in use is covered. The SOPs included on the following pages are only appropriate for a standard fiber optic Raman probe.

  • Page 56: Sop-5: Sample Positioning When Using A Fiber Optic Probe

    56 . RamanFlex 400 Series Getting Started Guide 7. Ensure that laser safety precautions are being observed, and then click Continue. If you have any doubts about the required safety precautions click Cancel. When the laser is on and the shutter is open the Laser Status icon on the Measurement bar will be updated to Class 3B Laser Status .

  • Page 57: Calibration And Performance Verification Sops

    Using the RamanFlex 400 Series . 57 Calibration and Performance Verification SOPs Several different automated calibration tests are included in the software: Wavelength (x-axis) calibration uses the position of precisely known lines in a neon • emission spectrum to calibrate the detector. •...

  • Page 58: Sop-7: Wavelength Calibration Verification

    When you have addressed these issues, repeat the wavelength calibration verification to ensure that the instrument has been brought back within specification. If the instrument fails the wavelength calibration verification again, please contact PerkinElmer. SOP-7: Wavelength Calibration Verification 1.

  • Page 59: Sop-8: Wavelength Calibration

    Using the RamanFlex 400 Series . 59 5. Focus on a sample of polystyrene (part number L1181228). 6. When the polystyrene sample is optimally focused, click to exit the Live display. 7. Select Toolbox on the Setup Instrument Advanced tab. The Adjustments Toolbox dialog is displayed.

  • Page 60: Sop-9: Laser Frequency Calibration

    60 . RamanFlex 400 Series Getting Started Guide 5. Select the Setup Instrument Advanced tab and click Toolbox. The Adjustments Toolbox dialog is displayed. 6. Select The Wavelength Calibration dialog is displayed. 7. Ensure that you have followed the instructions on the dialog, and then click Next. The instrument will now perform the wavelength calibration routine.

  • Page 61: Sop-10: Intensity Calibration

    15 °C – 20 °C or 25 °C – 30 °C, we recommend that you perform a NIST intensity correction at this new temperature. Contact your PerkinElmer Raman Business Unit if you wish to set up a NIST intensity correction for a different temperature range.
  • Page 62 62 . RamanFlex 400 Series Getting Started Guide 1. Ensure the RamanFlex 400 is switched on. If not, follow SOP-1. NOTE: If you have a RamanFlex 400F with two fiber optic probes, ensure that the probe not in use is covered. 2.

  • Page 63: Summary

    Using the RamanFlex 400 Series . 63 Summary The only calibration procedure that needs to be performed on a regular basis is the • wavelength calibration verification procedure. • The wavelength and laser frequency calibrations should only be performed if the instrument fails the wavelength calibration verification procedure.

  • Page 64: Additional Parts And Upgrades

    64 . RamanFlex 400 Series Getting Started Guide Additional Parts and Upgrades Safety and Calibration Part Description Number L1320262 Raman Laser Safety Kit (785 nm) Contains external interlock cable, laser glasses, laser detection card, and door warning label L1320237 Raman Calibration Kit (785 nm) Contains neon lamp and fiber optic connector (for wavelength calibration), calibration plate containing polystyrene (for wavelength calibration verification and laser wavelength calibration), calcite (for resolution verification), and NIST...

  • Page 65: Sers Consumables

    Ultra-long working distance lens adaptor for FOP – allows a 25 mm working distance. L1320095 Fiber probe sample holder (for measurement of samples in small vials and cuvettes) For information about customized working distance or cable length fiber optic probes, contact your PerkinElmer Sales Representative. SERS Consumables Part Description Number L1329300 SERS gold colloid –...
  • Page 66 66 . RamanFlex 400 Series Getting Started Guide...

  • Page 67: Maintenance And Troubleshooting

    Maintenance and Troubleshooting...

  • Page 68: Maintenance

    Avoid spilling liquid into the instrument. Clean all external spills immediately. If anything that is spilled enters the main body of the spectrometer, make the instrument inoperative and then contact a PerkinElmer Service Engineer. Do not directly clean electrical contacts.

  • Page 69: Cleaning The Air Filters

    Maintenance and Troubleshooting . 69 Cleaning the Air Filters The air filters are located inside the top panel of the spectrometer module. You should clean the filters regularly. To clean the filters: 1. Switch off the instrument power supply unit, and the deep-depletion CCD power supply, if applicable.

  • Page 70: Servicing The Spectrometer

    Service of the Fiber Optic Probe The FOP is designed to be maintenance free and should not be opened or adjusted by anyone other than a PerkinElmer Service Engineer. Contact PerkinElmer if you have any questions regarding maintenance or servicing of the FOP.

  • Page 71: Troubleshooting

    Maintenance and Troubleshooting . 71 Troubleshooting This section is a reference guide if you are having any problems with your instrument. Troubleshooting outlines what to do if your instrument or software malfunctions. Hardware Problem: During the installation procedure the USB devices are not recognized.

  • Page 72: Decontamination

    (8:30 a.m. – 7 p.m. EST) (+1) 203-925-4602 (outside the USA) Customer Care Canada: 800-561-4646 Customer Care EU: 0800 40 858 (Brussels) 0800 90 66 42 (Monza) If you are located outside of these regions, please call your local PerkinElmer sales office for more information.

  • Page 73: Weee Instructions For Perkinelmer Products

    Customer Care department in your region. http://las.perkinelmer.com/OneSource/Environmental-directives.htm Products from other manufacturers may also form a part of your PerkinElmer system. These other manufacturers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive. Please contact these manufacturers directly before discarding any of their products.
  • Page 74 74 . RamanFlex 400 Series Getting Started Guide...

Table of Contents