Biohit Proline Plus Instruction Manual page 15

8. 8. Read Read
9. 9. Repeat Repea
recorded. recorded.
1 1 0. 0. Convert the Convert the
V V = m = m
i i
1 1 1 1 . . Calculate the Calcu
v v
̄ ̄
= ( = (
1 1 2. 2. For conformity evaluation calculate the For conformity evaluation calculate the
the measurement: the measurement:
in in l: l:
μ μ
or or in in
13. For conformity evaluation calculate the random error of the 13. For conformity evaluation calculate the random error of the
measurement: measurement:
as standard deviation s = as standard deviation s =
or as coef or as coef
14. Compare the systematic error (inaccuracy) and random error 14. Compare the systematic error (inaccuracy) and random error
(imprecision) with the values in the (imprecision) with the values in the
- (p. 49) or the speci - (p. 49) or the speci
If the results fall within the speci If the results fall within the speci
for use. Otherwise check both systematic and random errors for use. Otherwise check both systematic and random errors
and, when necessary, proceed to the recalibration procedure and, when necessary, proceed to the recalibration procedure
(Chapter 11.2). (Chapter 11.2).
Note Note : Systematic error (inaccuracy) is the difference between the : Systematic error (inaccuracy) is the difference between the
dispensed volume and the selected test volume. Random error dispensed volume and the selected test volume. Random error
(imprecision) is the scatter of the dispensed volumes around the (imprecision) is the scatter of the dispensed volumes around the
mean of the dispensed volume. mean of the dispensed volume.
Note Note : Biohit speci : Biohit speci
tions (ISO 8655-6). The user should establish own speci tions (ISO 8655-6). The user should establish own speci
based on the based on the
the pipette (ISO8655-1). the pipette (ISO8655-1).
Table 1 Table 1
T T emp. emp. (°C) (°C)
20.0 20.0
20.5 20.5
21.0 21.0
21.5 21.5
22.0 22.0
22.5 22.5
23.0 23.0
23.5 23.5
Note: Note: This method is based on This method is based on
the the weight weight in in mgs mgs
t the the test test cycle cycle
recorde recorded masses
Z Z
i i
late the mean volume ( mean volume (
V V )/10 )/10
∑ ∑
i i
e e = =
s s
%: %: e e = 100 ( = 100 (
s s
fi fi cient of variation CV = 100 cient of variation CV = 100
fi fi cations are achieved in strictly controlled condi- cations are achieved in strictly controlled condi-
fi fi eld of use and the accuracy requirements placed on eld of use and the accuracy requirements placed on
95 95
1.0028 1.0028
1.0029 1.0029
1.0030 1.0030
1.0031 1.0031
1.0032 1.0032
1.0033 1.0033
1.0034 1.0034
1.0036 1.0036
(m (m ). ).
i i
until 1 until 10 0 measurements have measur
d masses (m (m ) to volumes ) to volumes (
i i
Z Z = = cor correc rection
v v
̄ ̄
) deliver ) delivered:
v v
̄ ̄
- V - V
s s
v v
̄ ̄
- V - V )/ )/V V
s s s s
v v
̄ ̄
( ( V V - - ) )
∑ ∑
i i
n - 1 n - 1
performance speci per
cations of your own laborator cations of your own
fi fi
fi fi cations, the pipette is ready cations, the pipette is ready
(ISO 8655-1.) (ISO 8655-1.)
Z-values Z-values ( ( l/mg): l/mg):
μ μ
Air Air Pressure Pressure
100 100
1.0028 1.0028
1.0029 1.0029
1.0031 1.0031
1.0032 1.0032
1.0033 1.0033
1.0034 1.0034
1.0035 1.0035
1.0036 1.0036
ISO 8655. ISO 8655.
ements have
(V V ) )
i i
tion factor factor ( ( T T able able
ed:
systematic error e systematic error e
V V = selected test volume = selected test volume
s s
n = number of n = number of
2 2
measurement (10) measurement (10)
v v
̄ ̄
/ /
s s
formance speci
laboratory
(kPa) (kPa)
101.3 101.3
1.0029 1.0029
1.0030 1.0030
1.0031 1.0031
1.0032 1.0032
1.0033 1.0033
1.0034 1.0034
1.0035 1.0035
1.0036 1.0036
been been
1 1 ) )
of of
s s
fi fi cations cations
y . .
fi fi cations cations
105 105
1.0029 1.0029
1.0030 1.0030
1.0031 1.0031
1.0032 1.0032
1.0033 1.0033
1.0034 1.0034
1.0036 1.0036
1.0037 1.0037
1 1 1 1