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Biohit Proline Plus Instruction Manual page 15

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8.
8.
Read
Read
9.
9.
Repeat
Repea
recorded.
recorded.
1
1
0.
0.
Convert the
Convert the
V
V
 = m
 = m
i
i
1
1
1
1
.
.
Calculate the
Calcu
v
v
    ̄
   ̄
 
 
 = ( 
 = ( 
1
1
2.
2.
For conformity evaluation calculate the
For conformity evaluation calculate the
the measurement:
the measurement:
 
 
in
in
l:
l:
μ
μ
 
 
or
or
in
in
13. For conformity evaluation calculate the random error of the
13. For conformity evaluation calculate the random error of the
measurement:
measurement:
 
 
as standard deviation s =
as standard deviation s =
 
 
or as coef
or as coef
14. Compare the systematic error (inaccuracy) and random error
14. Compare the systematic error (inaccuracy) and random error
(imprecision) with the values in the
(imprecision) with the values in the
- (p. 49) or the speci
- (p. 49) or the speci
If the results fall within the speci
If the results fall within the speci
for use. Otherwise check both systematic and random errors
for use. Otherwise check both systematic and random errors
and, when necessary, proceed to the recalibration procedure
and, when necessary, proceed to the recalibration procedure
(Chapter 11.2).
(Chapter 11.2).
Note
Note
: Systematic error (inaccuracy) is the difference between the
: Systematic error (inaccuracy) is the difference between the
dispensed volume and the selected test volume. Random error
dispensed volume and the selected test volume. Random error
(imprecision) is the scatter of the dispensed volumes around the
(imprecision) is the scatter of the dispensed volumes around the
mean of the dispensed volume.
mean of the dispensed volume.
Note
Note
: Biohit speci
: Biohit speci
tions (ISO 8655-6). The user should establish own speci
tions (ISO 8655-6). The user should establish own speci
based on the
based on the
the pipette (ISO8655-1).
the pipette (ISO8655-1).
Table 1
Table 1
 
 
T
T
emp.
emp.
(°C)
(°C)
 
 
 
 
20.0
20.0
20.5
20.5
 
 
21.0
21.0
 
 
21.5
21.5
 
 
22.0
22.0
 
 
22.5
22.5
 
 
23.0
23.0
 
 
23.5
23.5
Note:
Note:
 This method is based on
 This method is based on
the
the
weight
weight
in
in
mgs
mgs
t
the
the
test
test
cycle
cycle
recorde
recorded masses
 
 
Z
Z
i
i
late the
mean volume ( 
mean volume ( 
V
V
 )/10
 )/10
i
i
e
e
=
=
s
s
%:
%:
e
e
 = 100 ( 
 = 100 ( 
s
s
cient of variation CV = 100
cient of variation CV = 100
cations are achieved in strictly controlled condi-
cations are achieved in strictly controlled condi-
eld of use and the accuracy requirements placed on
eld of use and the accuracy requirements placed on
95
95
1.0028
1.0028
1.0029
1.0029
1.0030
1.0030
1.0031
1.0031
1.0032
1.0032
1.0033
1.0033
1.0034
1.0034
1.0036
1.0036
(m
(m
 ).
 ).
i
i
until 1
until
10
0
measurements have
measur
d masses
(m
(m
 ) to volumes
 ) to volumes (
i
i
Z
Z
=
=
cor
correc
rection
v
v
    ̄
   ̄
 ) deliver
 )
delivered:
v
v
    ̄
   ̄
 - V
 - V
 
 
s
s
v
v
   ̄
    ̄
 - V
 - V
 )/  )/V
V
s
s
s
s
v
v
    ̄
   ̄
(
(
V
V
-
-
 )
 )
 
 
i
i
n - 1
n - 1
 
 
performance speci
per
cations of your own laborator
cations of your own
cations, the pipette is ready
cations, the pipette is ready
(ISO 8655-1.)
(ISO 8655-1.)
Z-values
Z-values
l/mg):
l/mg):
μ
μ
Air
Air
Pressure
Pressure
100
100
1.0028
1.0028
1.0029
1.0029
1.0031
1.0031
1.0032
1.0032
1.0033
1.0033
1.0034
1.0034
1.0035
1.0035
1.0036
1.0036
ISO 8655.
ISO 8655.
ements have
(V
V
 )
 )
i
i
tion
factor
factor
(
(
T
T
able
able
ed:
systematic error e
systematic error e
V
V
= selected test volume
= selected test volume
 
 
s
s
 
 
n = number of
n = number of
2
2
measurement (10)
measurement (10)
v
v
    ̄
   ̄
 / 
 / 
s
s
formance speci
laboratory
(kPa)
(kPa)
101.3
101.3
1.0029
1.0029
1.0030
1.0030
1.0031
1.0031
1.0032
1.0032
1.0033
1.0033
1.0034
1.0034
1.0035
1.0035
1.0036
1.0036
been
been
1
1
)
)
 of
 of
s
s
cations
cations
y
.
.
cations
cations
105
105
1.0029
1.0029
1.0030
1.0030
1.0031
1.0031
1.0032
1.0032
1.0033
1.0033
1.0034
1.0034
1.0036
1.0036
1.0037
1.0037
1
1
1
1

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