8.2
8.2.
.
Rev
Reverse
1
1
.
.
Fit
Fit
the
the
2.
2.
Press
Press
3.
3.
Place the
Place the
and smoothly release the operating button up to the starting
and smoothly release the operating button up to the starting
position.
position.
4.
4.
Withdraw
Withdraw
the container to remove excess.
the container to remove excess.
5.
5.
Press
Press
the present volume. Hold the
the present volume. Hold the oper
The
The
delivery.
delivery.
6.
6.
Discard
Discard
to the second stop.
to the second stop.
8.3.
8.3.
Repetitive reverse techniq
Repetiti
1
1
.
.
Follow
Follow
2.
2.
Continue
Continue pipettin
needed.
needed.
3.
3.
Finally
Finally
button to the
button to the secon
9.
9.
RECOMME
RECOMMENDA
•
•
Make
Make
•
•
Hold
Hold
the tip only a few millimeters into the
the tip only a few millimeters into the
•
•
Always
Always
•
•
Pre-rinse
Pre-rinse
emptying the tip for three to
emptying the tip for three to
especially when
especially when
greater than water or liquids with high vapor pressure (e.g.
greater than water or liquids with high vapor pressure (e.g.
ethanol).
ethanol).
•
•
Check
Check
temperature.
temperature.
•
•
When
When
ambient temperature change the tip after each pipetting. Do
ambient temperature change the tip after each pipetting. Do
not pre-rinse the tip.
not pre-rinse the tip.
•
•
T
T
o
o
avoid
avoid
especially with the tip attached.
especially with the tip attached.
•
•
Change
Change
-250 pipetting cycles).
-250 pipetting cycles).
erse
pipetting
pipetting
tip
tip
onto
onto
the
the
pipette
pipet
the
the
operating button
operating button
tip
tip
just under
just under
the
the
tip
tip
from
from
the
the
operating
operating
liquid that remains in t
liquid that remains in
the
the
remaining
remaining
Fi Fig.
g.
7
7
.
.
0
0
1
1
2
2
ve reverse
the
the
reverse
rever
se
techni
technique
pipetting
g
discard
discard
the
the
remaining
remaining
second stop.
d stop.
NDATIONS
TIONS
sure
sure
that
that
the
the
tip
tip
the
the
pipette
pipette
vertica
vertically
control
control
the
the
operating
operating
the
the
tip
tip
before
before
pipetting liquids
pipetting liquids
that
that
the
the
pipette,
pipette,
pipetting
pipetting
liquids
liquids
contamination,
contamin
ation,
the
the
tip
tip
cone
cone
(Fig. 7)
(Fig. 7)
te
tip
tip
cone.
cone.
all
all
the
the
way
way
the
the
surface of
sur
face of
the
the
liquid
liquid
touching
touching
button
button
smoothly
smoothly
operating button at the
ating button at the
the tip should
he tip should
liquid
liquid
by
by
pressing
pressing
technique
ue
que
steps
steps
by
by
repeating steps
repea
ting steps
liquid
liquid
FOR
FOR
GOOD
GOOD
is
is
fi
fi
rmly attache
rmly
attached to the tip
lly
when
when
aspirating
aspirating
button
button
aspirating
aspirating
fi
fi
ve times. This is important
ve times. This is important
with
with
tip
tip
and
and
with
with
temperatures
temperature
do
do
not
not
rest
rest
fi
fi
lter regularly (recommendation after 50
lter regularly (recommendation after 50
to
to
the
the
second st
second
the liquid
the liquid
against the
against the
to
to
the
the
fi
fi
rst stop to deliver
rst stop to deliver
not be included in
not be included in
the
the
operating
operating
1
1
to
to
5.
5.
3
3
to
to
5 as
5 as
by
by
pressing
pressing
the
the
PIPETTING
PIPET
TING
d to the tip
cone.
cone.
the
the
liquid
liquid
liquid.
liquid.
slowly
slowly
and
and
smoothly.
smoothly
the
the
liquid
liquid
by
by
a
a
viscosity and
viscosity and
liquid
liquid
are at
are at
s
differe
different
the
the
pipette
pipette
stop.
op.
(2-3
(2-3
mm)
mm)
edge
edge o
of
f
fi
fi
rst stop.
rst stop.
the
the
button
button
long as
long as
operating
operating
and
and
place
place
.
lling and
lling and
fi
fi
density
density
the
the
same
same
nt
to
to
the
the
on
on
its
its
side
side
7
7
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