Reverse Pipetti Pipetting - Biohit Proline Plus Instruction Manual

The Safe-Cone The Safe-Cone
is recommended to use the Standard is recommended to use the Standard
and the Plus and the Plus
culture, bacterial and virological work and molecular biology. culture, bacterial and virological work and molecular biology.
Filters need to be changed regularly. The interval for filter Filters need to be changed regularly. The interval for filter
changing is application dependant but the recommendation is to changing is application dependant but the recommendation is to
change the change the
pipetting cycles) and always in case pipetting cycles) and always in case
of over-aspiration. (See Chapter 2 of over-aspiration. (See Chapter 2
for ordering information.) The Biohit for ordering information.) The Biohit
Filter Foreceps should be used to Filter Foreceps should be used to
avoid touching the soiled filters avoid touching the soiled filters
by hand (Fig.5). See Chapter 2 for by hand (Fig.5). See Chapter 2 for
ordering information. Make sure that ordering information. Make sure that
the the fi fi lter is disposed into a suitable lter is disposed into a suitable
waste container. Clean the tip cone waste container. Clean the tip cone
if needed and put the new if needed and put the new
place. place.
8. 8. PIPETTING PIPETTING
T T wo basic pipetting techniques, for wo basic pipetting techniques,
associated with Proline associated with Proline
most common used pipetting technique. The technique employs most common used pipetting technique. The technique employs
the blow-out function ensuring complete delivery of the liquid. the blow-out function ensuring complete delivery of the liquid.
Reverse pipetting Reverse pipetting
or foaming liquids, or very small volumes of liquid. A selected or foaming liquids, or very small volumes of liquid. A selected
volume plus an excess is volume plus an excess is aspira
without blow-out, and, thus, the without blow-out, and, thus, the
The reverse technique also facili The reverse technique also
same volume. If reverse pipetting technique is used the pipette same volume. If reverse pipetting technique is used the pipette
might need recalibration. might need recalibration.
8. 8.1 1 . For . Forward pipet
1 1 . . Fit Fit the the
2. 2. Press Press
3. 3. Place the Place the
and smoothly release the operating button up to the starting and smoothly release the operating button up to the starting
position. Wait one seco position. Wait one
liquid, touching against the edge of the container to remove liquid, touching against the edge of the container to remove
excess from the outside of the excess from the outside of the
4. 4. Dispense the Dispense the
fi fi rst stop. After a rst stop. After a shor
second stop. This action will empty the tip. second stop. This action will empty the tip.
6 6
fi fi lters are available as Standard or Plus versions. It lters are available as Standard or Plus versions. It
fi fi lter for more demanding applications such as cell lter for more demanding applications such as cell
lter daily (after 50 - 250 lter daily (after 50 - 250
fi fi
TECHNIQUES TECHNIQUES
is recommended for highly viscous, biological is recommended for highly viscous, biological
ward pipetting (Fig. 6) ting (Fig. 6)
tip tip onto onto the the pipet pipette
the the operating operating
tip tip just under just under
second. Carefully withdraw the tip from the
liquid liquid
short delay press the operating but
Fig. Fig. 6. 6. 0 0
1 1
2 2
lter for general applications lter for general applications
fi fi
lter in lter in
fi fi
forward and reverse pipetting, a ward and reverse pipetting,
® ®
Plus pipettes. Plus pipettes.
aspirated into the tip. ted into the tip.
excess volume remains in excess volume remains in
facilitates the tates the
te tip tip cone. cone.
button button to to the the fi fi
the the surface of sur face of
nd. Carefully withdraw the tip from the
tip. tip.
by by pressing the pressing the
t delay press the operating
Forward pipetting Forward pipetting
The The deliver delivery is done
repeated delivery repeated delivery
rst stop. rst stop.
the liquid the liquid
operating button operating button
button to the
Fig. 5. Fig. 5.
are re
is the is the
y is done
the tip. the tip.
of the of the
(2-3 (2-3 mm) mm)
to to the the
ton to the