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Pipettes
Pipettes
Unscrew the tip cone cylinder (3) counterclockwise
Unscrew the tip cone cylinder (3) counterclockwise
4.
4.
Clean the
Clean the
(cylinder) and the piston (4) with Biohit Proline Biocontrol,
(cylinder) and the piston (4) with Biohit Proline Biocontrol,
ethanol (70%), isopropanol (60%) or mild detergent and soft
ethanol (70%), isopropanol (60%) or mild detergent and soft
lint-
lint-free
free
5.
5.
Clean the
Clean the
(cylinder) with a cotton swab. Be careful with the pipettes so
(cylinder) with a cotton swab. Be careful with the pipettes so
that the seal inside the tip cone will not damage.
that the seal inside the tip cone will not damage.
6.
6.
Rinse
Rinse
dry.
dry.
7.
7.
Pipettes
Pipettes
Put a thin layer of grease on the piston (4).
Put a thin layer of grease on the piston (4).
Pipettes
Pipette
Put a thin layer of grease around the piston seal (4).
Put a thin layer of grease around the piston seal (4).
Pipettes 2000, 5000 a
Pipettes 2000, 5000
Put a thin layer of grease
Put a thin layer of grease
of the tip cone cylinder (3).
of the tip cone cylinder (3).
Note
Note
: Avoid excess grease. Use only the grease provided with
: Avoid excess grease. Use only the grease provided with
the pipette.
the pipette.
Note
Note
: Before reassembling check that no lint or particles are on
: Before reassembling check that no lint or particles are on
the surface
the surface
Decontamination:
Decontamination:
For complete decontamination of the lower parts place the tip
For complete decontamination of the lower parts place the tip
ejector collar (1), tip cone holder (2) and tip cone (3) into a beaker
ejector collar (1), tip cone holder (2) and tip cone (3) into a beaker
containing Biohit
containing Biohit
minutes.
minutes. Wip
lint-
lint-free cloth. Rinse
free cloth. Rinse
par
parts dry. Grease the piston and
ts dry. Grease the piston and
given earlie
given
earlier in this chapter.
Reassembling:
Reassembling:
1. Pipettes:
1. Pipettes:
Carefully place the tip cone (3) on the piston and attach it by
Carefully place the tip cone (3) on the piston and attach it by
screwing the tip cone holder (2) clock
screwing the tip cone holder (2)
Pipettes
Pipettes
Carefully plac
Carefully
clockwise. Make sure that the tip cone (cylinder) is properly
clockwise. Make sure that the tip cone (cylinder) is properly
tightened. Avoid over tightening.
tightened. Avoid over tightening.
2.
2.
Attach
Attach
3.
3.
Place
Place
4.
4.
Press
Press
grease
grease ha
5.
5.
Check
Check
Note
Note
: It is always necessary to check the performance of the
: It is always necessary to check the performance of the
pipette after in-house service or maintenance.
pipette after in-house service or maintenance.
10.3. Sterilization of the pipette
10.3. Sterilization of the pipette
The entire Proline
The entire Proline
autoclaving at 121°C, (252°F), 1 atm for 20 minutes. Remove the
autoclaving at 121°C, (252°F), 1 atm for 20 minutes. Remove the
2000,
2000,
5000,
5000,
tip
tip
ejector collar,
eje
ctor collar,
cloth.
cloth.
interior
interi
or
of
of
the
the
parts with
parts with
100
100
l:
l:
≤
≤
μ
μ
s
250,
250,
500
500
and
and
of the piston.
of the piston.
Proline
Proline
Wipe the
e the
piston
piston
the
the par
parts with distilled or
r in this chapter.
2000,
2000,
5000
5000
place the tip cone cylinder (3) on the piston
e the tip cone cylinder (3) on the piston
the
the
tip
tip
ejector collar
ejector collar
the
the
new
new
tip
tip
cone
cone
the
the
operating button
operating button
has spread
s spread
evenly.
evenly
the
the
per
performance
formance
®
®
Plus pipette can be sterilized by steam
Plus pipette can be sterilized by steam
1
1
0
0
000
000
l:
l:
μ
μ
the
the
tip
tip
the
the
tip
tip
ejector
ejec
tor
distilled
distilled
water
water
if
if
1000
1000
l:
l:
μ
μ
and 1
nd
10 000
0 000
l:
l:
μ
μ
on the piston seal
on the piston seal
Biocontrol and
Biocontrol and
with Biohit
with Biohit
Proline
Proline
ts with distilled or
seal according to the instructions
seal according to the instructions
clockwise.
and
and
1
1
0
0
000
000
μ
μ
l:
l:
(1 (1)
)
by
by
screwing
screwing
lter on its place.
lter on its place.
fi
fi
several
several
.
of
of
the
the
pipette.
pipette.
and remove it.
and remove it.
cone holder,
cone holder,
the
the
colla
collar
r
and
and
the
the
needed and
needed and
let
let
(4 (4) and the interio
) and the
leave
leave
for
for
at least
at least
Biocontrol and the
Biocontrol and the
sterile
sterile water
water. Let the
wise.
it
it
clockwise.
clockwise.
times
times
to
to
ensure that
ensure that
tip
tip
cone
cone
tip
tip
cone
cone
the
the
parts
par
ts
interior
r
30
30
. Let the
and screw
and screw
the
the
9
9
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