The ePET® pipette is intended to be used in liquid handling applications for dispensing liquids in the volume range of 0,2 μl to 5 ml. With ePET® pipettes it is recommended to use Sartorius Optifit Tips or SafetySpace™ Filter Tips to ensure the best compatibility and performance.
Optifit Tips and SafetySpace™ Filter Tips are also available as Low Retention Tips. 4. Unpacking The ePET® pipette package contains the following items: 1. Pipette 2. Grease 3. AC/DC Adapter (acts as a recharging unit) 4.
4.2. Charging the Pipette An ON/OFF switch is located at the top of the Connect the pipette. This switch protects the battery from charger to the discharging while the pipette is not in use and pipette's charger not connected to the charger. port 1.
5. Pipette Parts and Materials Charger port ePET® Single-Channel cover (PC) Finger support (at the back) (ASA/PC) Display (glass) Keyboard (silicon keys) Start button (POM) Tip ejector lever (POM) Handle (ASA/PC) Power ON/OFF switch Tip ejector collar (PP) ePET® Multichannel...
6. Pipette Description The control and the programming of the ePET® electronic pipette are performed using the Power Switch control keys and display. Charger Port 6.1. Controls - Power Switch - Charger Connection - Battery Compartment Control Keys: S Changes speed Moves to next mode ...
6.5. Loading and Ejecting Tips is loaded tightly Original Sartorius tips are recommended for use with Sartorius ePET® pipettes. Before fitting a tip make sure that the pipette tip cone is clean. Press the tip onto the cone of the pipette firmly, to ensure an airtight seal.
7. Programming the Pipette Programming is performed using the six-key keyboard and the LC-display. There are three operating modes offering special functions and varying speeds to choose from. 7.1. Mode Selection and Mode Recall 1. Press repeatedly to view the available modes of the pipette.
7.3. Pipetting Mode (P) The pipette performs ordinary pipetting with blow-out. 1. Press to display ↑ 2. Press to confirm the mode change. 3. Select the desired pipetting volume by using Step 5 Aspiration to increase, and to decrease. (Pipetting Mode) ...
7.4. Multi-Dispensing Mode (d) In the Multi-Dispensing Mode (d), the pipette performs repetitive dispensings of a selected ↑ volume. During this operation, the desired volume, plus the automatically selected excess volume, is aspirated into the tip. Step 7 Aspiration 1. Press to display (Multi-Dispensing Mode)
7.5. Diluting Mode (dd) In the Diluting Mode (dd), two different solutions ↑ separated by an air gap are aspirated and then dispensed together with an automatic blow- out. The purpose of the air gap is to prevent contamination while aspirating the second volume, but it will not prevent the two liquids Step 7 Aspiration from mixing in the tip.
7.6. Mixing Mode with Pipetting or Diluting (*) In the Mixing Mode, the piston is automatically moved up and down to mix the liquid in the delivery vessel. The mixing time is controlled by the START button. 1. Use to select either on the display.
To Mix: 1. Position the tip in the solution, then press ↕ and hold the START button. The mixing is done automatically as long as the START button is held down. 2. Position the tip to dispense. Press the START button twice.
- Change the filter on the tip cone regularly (after 50 - 250 pipettings). 9. Storage When not in use, it is recommended that the pipette is stored on a Sartorius Linear stand, ® T i t i connected to the charger. The green charging light should be lit.
10. Performance Testing It is recommended, the performance of your ePET® pipettes be checked regularly (e.g. every 3 months), and always after in-house maintenance. However, the user should establish a regular testing routine for their pipettes with regard to accuracy requirements of the application,...
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(ISO 8655-1.) NOTE: Sartorius specifications are achieved in strictly controlled conditions (ISO 8655-6). The user should establish own specifications based on the field of use and the accuracy requirements placed on the pipette (ISO8655-1).
11. Maintenance To maintain the best results with your ePET® pipette, the cleanliness of every unit should be checked daily. Particular attention should be paid to the tip cone(s). ePET® pipettes have been designed for easy in-house service. However, Sartorius also provides...
Test the operation of the tip ejector. NOTE: Check the performance of your Sartorius ePET® pipette regularly, e.g. every 3 months, and always after in-house service or maintenance. 11.3. Battery Replacement If the battery is not sufficiently charged for proper operation of the pipette, follow these steps for replacement of the battery.
12. Troubleshooting The ePET® electronic pipette has a built-in monitoring program to control the performance of each pipetting action. If the error message ER1 appears on the display, this means the pipette has been unable to perform the attempted action properly.
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Troubles, causes and solutions Trouble Possible cause Solution Droplets left Unsuitable tip Use original Sartorius tips inside the tip Non-uniform wetting of the Attach new tip tip plastic Laeakage or pipetted Tip incorrectly attached Attach firmly volume Unsuitable tip Use original Sartorius tips...
The Sartorius Quality Assurance Procedure guarantees that the ePET® electronic pipette you have purchased is ready for use. Each ePET® electronic pipette is CE marked, fulfilling the requirements of the EN 55014, 1993/EN 55104, 1995/ ISO 13485:2003 and Directive (98/79 EC).
The manufacturer's specifications should be used as guidelines when establishing your own performance specifications in accordance with ISO 8655. Specifications ePET® Single and 8-Channel Pipettes Order No. Channels Volume Test Systematic Random range µl...