Sartorius Tacta User Manual
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Sartorius Tacta User Manual

Mechanical pipette
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Summary of Contents for Sartorius Tacta

  • Page 1 Tacta® Mechanical Pipette User Manual...

  • Page 2: Table Of Contents

    Table of Contents 1. Introduction ................................................... 3 1.1 Intended Use ................................................3 1.2 Pipette Parts and Materials ........................................... 3 1.3 Sartorius Pipette Tips ............................................4 1.4 Adjustment Tool ..............................................4 2. Package Contents ................................................. 4 3. Tagging the Pipette ..............................................5 4.

  • Page 3: Introduction

    Directive 98/97/EC of the European Parliament. The Tacta® product range covers a volume range of 0.1 μl to 10 ml. It is recommended that Sartorius Optifit Tips or SafetySpace Filter Tips are used with Sartorius pipettes to ensure optimum compatibility and performance.

  • Page 4: Sartorius Pipette Tips

    Sartorius Pipette Tips We recommend only using Sartorius pipette tips with the Tacta® pipette. Using fully compatible tips guarantees the pipette’s performance specifications and ensures pipetting accuracy and precision. Sartorius tips are made of pure virgin polypropylene and manufactured in protected cleanroom conditions.

  • Page 5: Tagging The Pipette

    3. Tagging the Pipette You can use personalized tags to make each Tacta® pipette easily identifiable. NOTE: If you intend to autoclave the pipette, make sure you use suitably resistant ink. 1. Write the identifying information on the tag. 2. Attach the tag to the finger support.

  • Page 6: Using The Carousel Stand Adapter

    Using the Carousel Stand Adapter The Carousel Stand (Order Code 725600) is made compatible with Tacta® by using the adapter provided in the Tacta® package. NOTE: A Carousel Stand that holds six Tacta® pipettes is available separately see 10.1 Order Information and Specifications.

  • Page 7: Attaching And Ejecting Tips

    If the volume is unlocked (the red bar is visible): 1. Turn the operating button to adjust the volume. 2. Slide the volume lock button down. Volume adjustment is now locked, preventing accidental volume changes.  Always lock after setting the volume NOTE: If the volume lock is not used, volume can be accidentally changed.
  • Page 8 NOTE: Do not force the pipette into the tip, or rock it from side to side, or front to back when attaching tips.
  • Page 9 To eject a tip: 1. Hold the pipette over a suitable waste container. 2. Press the tip ejector with your thumb.

  • Page 10: Using Protective Tip Cone Filters

    Using Protective Tip Cone Filters Replaceable Sartorius Safe-Cone Filters can be used with your pipette to help prevent liquids and aerosols from entering the pipette housing, and causing contamination or damage. We recommend you always use these filters when using non-filter tips. The operating button allows safe and easy filter ejection.

  • Page 11: Operating The Pipette

    6. Operating the Pipette The Tacta® pipette is generally used for forward and reverse pipetting. Good Pipetting Practices − Make sure the tip is firmly attached to the tip cone(s). − Pre-rinse the tip before aspirating by filling and emptying the tip three to five times. This is particularly important when pipetting liquids with a viscosity and density greater than water, or volatile liquids with high vapor pressure (such as ethanol).

  • Page 12: Reverse Pipetting

    7. Maintaining the Pipette The Tacta® pipette range has been designed for easy in-house service. If the pipette is in daily use, we recommend you clean and decontaminate it and check its performance every three months. Sartorius also provides a complete repair and calibration service, including a service report and performance certificate.

  • Page 13: Cleaning The Outer Surface Of The Pipette (Daily)

    Cleaning the Outer Surface of the Pipette (daily) Your Tacta® pipette should be checked daily to ensure it is clean. To clean and decontaminate the outer surface of the pipette, use a disinfectant liquid or mild detergent and a soft, lint-free cloth.
  • Page 14 b. With a 5000 μl pipette, hold the locking ring (2) firmly with your fingers and turn the tip cone cylinder counterclockwise with the other hand (see image below). Do not turn the locking ring as you turn the tip cone (3) as this will break the pipette. c.

  • Page 15: Sterilizing The Pipette

    8. Testing and Adjusting the Pipette We recommend you check the performance of your Tacta® pipette regularly (we suggest, every three (3) months) and always after in- house maintenance. Establish a regular testing routine that takes account of the following: −...

  • Page 16: Testing Pipette Performance

    (imprecision) is the scatter of dispensed volumes around the mean dispensed volume (ISO 8655-1). NOTE: Sartorius specifications are achieved in strictly controlled conditions (ISO 8655-6). We recommend establishing your own specifications based on the field of use and the accuracy requirements placed on the pipette (ISO8655-1).

  • Page 17: Adjusting The Pipette

    Adjusting the Pipette Your Tacta® pipette has been factory-checked using the forward pipetting technique and certified at 22°C using grade-3 distilled water according to ISO 3696. The calibration is based on ISO 8655-6: Gravimetric test method for volumetric instruments. In the following situations factory calibration is not applicable and the pipette will need adjusting: −...

  • Page 18: Troubleshooting

    - Foreign particles between the tip and cone - Clean tip cone, attach new tip - Tip cone holder incorrectly tightened - Tighten the tip cone holder - Pipette damaged - Return to your Sartorius service center for servicing Pipette outside established - Incorrect operation...

  • Page 19: Order Information

    10. Order Information Order Information and Specifications These specifications are only guaranteed when original Sartorius tips are used. The specifications should be used as guidelines when establishing your own performance specifications. Table 4: Tacta® pipette specifications Order Code Channels Volume Range...

  • Page 20: Warranty Information

    11. Warranty Information The Tacta® pipette is guaranteed for two years against defective materials or workmanship. If your Tacta® pipette fails to function at any time, contact your local Sartorius representative. ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT IS FOUND TO HAVE...
  • Page 22 The status of the information, specifications, and illustrations in this manual are subject to change. Sartorius reserves the right to make changes to the technology, features, specifications, and design of the equipment without notice. All trademarks are Sartorius property unless otherwise stated.

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