Hologic Compass ASY-05576 Operator's Manual page 47

Staining Protocol, 95% Reagent Alcohol Fixed ThinPrep
for use with ThinPrep Imaging
Equipment: Hologic Compass Stainer
Run no more than four slide racks together. Wait for all four to be unloaded before adding new racks.
Caution: Change the Distilled / Instrument Feed Water bath at Station 5 after every four racks of slides.
Maintain bath heights to completely cover the slides at full immersion.
Note:
Bath fill volume = 360 ml.
Station Time
LOAD n/a
1 01'00"
2 01'00"
3 01'00"
4 07'00"
5 00'10"
6 01'00"
7 00'30"
8 00'30"
9 00'30"
10 00'30"
11 00'30"
12 02'00"
13 00'15"
14 00'15"
15 04'00"
16 01'00"
17 01'00"
18 00'30"
19 00'30"
20 00'30"
21 01'00"
22 03'00"
UNLOAD n/a
a. Hologic specification for IFW: ≥1.0 megohm-cm Resistivity OR ≤1.0 μSiemens/cm Conductivity (Refer to Clinical and Laboratory Standards
Institute (CLSI) document C3-A4, 2006)
b. See ThinPrep Stain User's Manual, Section 1, "STAINING" or contact Hologic for current list of Hologic approved clearing agents and mounting
medias
Agitation: Dips = 10, Height = 30 mm
Duration
n/a 95% Reagent or Ethyl alcohol
Non-critical 70% Reagent or Ethyl alcohol
Non-critical 50% Reagent or Ethyl alcohol
Non-critical Distilled or Instrument Feed Water (IFW)
Critical ThinPrep Nuclear Stain
Critical Distilled or Instrument Feed Water (IFW)
racks.)
Critical ThinPrep Rinse Solution
Non-critical Distilled or Instrument Feed Water (IFW)
Critical ThinPrep Bluing Solution
Non-critical Distilled or Instrument Feed Water (IFW)
Non-critical 50% Reagent or Ethyl alcohol
Non-critical 95% Reagent or Ethyl alcohol
Critical ThinPrep Orange G Solution
Critical 95% Reagent or Ethyl alcohol
Critical 95% Reagent or Ethyl alcohol
Critical ThinPrep EA Solution
Critical 95% Reagent or Ethyl alcohol
Critical 95% Reagent or Ethyl alcohol
Non-critical 100% Reagent or Ethyl alcohol
Non-critical 100% Reagent or Ethyl alcohol
Non-critical 100% Reagent or Ethyl alcohol
Non-critical Xylene or other Hologic approved clearing agent
Non-critical Xylene or other Hologic approved clearing agent
n/a Xylene or other Hologic approved clearing agent
™
Compass Stainer Operator's Manual
®
Slides
Exit speed = Normal
Solution
a
a
(Replace after every 4
a
a
b
b
b
page 45