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Support: Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland on case-to-case basis): Phone: +49 30 809727-111 (workdays 9-17h CET)
These operating instructions are an integral part of the device. It must be kept in the immediate vicinity of the device and accessible to the user at all times. You can download these and other instructions from the KNAUER web- site: www.knauer.net/library...
1.4.4 Warranty seal A blue or orange warranty seal is affixed to some devices. A blue seal is used by KNAUER’s Manufacturing or Customer Support for devices to be sold. After repair, service technicians attach an orange seal onto the identi- ...
Operation in potentially explosive areas without special and additio- nal explosion protection. Contact the KNAUER Customer Support for more information. User qualification The user is qualified to handle the device if all of the following points apply: He has at least a basic knowledge of liquid chromatography.
Basic safety instructions Operator responsibility The operator is any person who operates the device himself or leaves it to a third party for use and who bears the legal product responsibility for the protection of the user or third parties during operation. The obligations of the operator are listed below: Know and follow the applicable work safety regulations ...
Basic safety instructions Working with solvents 2.5.1 General requirements The user is trained for handling different solvents. Note recommended solvents and concentrations in these instructions in order to avoid personal injury or damage to the device. For exam- ple, certain chemicals may cause PEEK capillaries to swell or burst (see chapter 12 on page 60).
system in order to avoid dead volumes, not exactly fitting connections and spreading contamination. Safety features: The device may only be opened by the KNAUER Cus- tomer Support of KNAUER or any company authorized by KNAUER (see chapter 1.4.1 on page 2).
Devices which are shipped without the completed document “Service request form and decontamination report” will not be repaired. If you would like to return a device to KNAUER, make sure to enclose the com- pleted document: www.knauer.net/servicerequest BlueShadow Pump 40P Instructions V7613...
Product information 3. Product information Views 3.1.1 Front view Legend Display Pressure sensor Ventilation screw Keypad Tube connections for the piston backflushing Pump head Fig. 1: Front view 3.1.2 Rear view Legend Warranty seal Space for rear label (with serial number) Fan opening Warning 1 Power switch...
Product information Features Dual-piston technology combined with optimized electronic pulsation dampen ing and compressibility compensation Liquid transport with low pulsation, stable flow rate and high flow accuracy Pump head made of stainless steel or with ceramic inlays Automatic piston backflushing ...
A warranty seal is affixed to some devices. For more information see chap. 1.4.4 on p. 2. Scope of delivery Note: Only use original parts and accessories made by KNAUER or a company authorized by KNAUER. Power cable BlueShadow Pump 40P with pump head ...
Installation and initial startup 4. Installation and initial startup Before you determine the operation site, read the chapter “Technical data” (see chapter 11 on page 58). There you will find all device-speci- fic information on power supply, ambient conditions and humidity. Note: The intended use be ensured only if the requirements for ambient conditions of the operating environment are met.
Country-specific plugs: Before switching on the device, check whether the supplied plug is approved for your country. Overview of the de- vice- and country-specific plug types from KNAUER: www.knauer.net/plugs. Power strips: If several devices are connected to one power strip, al- ...
Installation and initial startup Electric connections Use the Events and Remote pin header to connect the pump with ex- ternal devices. Use the LAN connection to connect the pump with external devices within a net work. Alternatively, connect the pump to a computer with the RS-232 port. ...
Installation and initial startup Process 1. Push the depressor tool into an upper small opening on the front of the terminal strip 2. Lead the cable into the opening below the inserted operating tool. 3. Remove the depressor tool. Next steps Check if the cables are firmly connected.
Installation and initial startup Signal Explanations ANALOG: Analog output signal reflecting either the measu- red system pressure or a control voltage for Pump The output range can be set to values of max. 1, 2, 5 and 10 V. ANALOG: IN Analog input signal for controlling the flow rate, e.g.: 1 V for 1 ml/min in the case of the 10 ml pump ...
Installation and initial startup 4.5.1 Connecting the solvent bottles To connect the solvent bottles, tubing with pre-installed solvent filters is used. The tubing is connected to the device with flangeless fittings. Note: Do not use any tools to tighten the fittings. Procedure Process Figure...
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Installation and initial startup Procedure Process Figure 1. Slide the flangeless fitting over the tubing. 2. Insert the tubing into the free inlet on the bottom of the pump head. 3. Tighten the fitting by hand. Fig. 8: Solvent line on pump head Next step Integrate the pump into the HPLC system.
Fig. 9: Connectors on rear side Note: HPLC devices from KNAUER only work with IP addresses, which have been assigned by IPv4. IPv6 is not supported. This chapter describes how to set up a chromatography system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network.
Installation and initial startup Process Procedure 1. On the computer, go to the control panel and check the LAN properties. 2. Hook up the router to the devices and the computer. 3. Set up the router for the computer network. 4.
Set the router properties (see chapter 4.6.4 on page 20). 4.6.4 Configuring the router The router is preset at the factory. Information about address, user name and password is noted in the router manual: www.knauer.net/router. Process Procedure 1. To open the router configuration, start your Internet browser and enter the IP address (does not apply for all routers).
Installation and initial startup 4.6.5 Integrating LAN into the company network A network administrator can integrate the LAN into your company net- work. In this case you use the WAN port of the router. Prerequisites There is a patch cable for the connection. ...
Installation and initial startup 4.7.1 Firmware Wizard: Setting a static IP address Note: More information about LAN settings can be found in the Mobile Control Software Instructions in the chapter "Firmware Wizard” (docu- ment no. V6851-2). Text box for serial number of the device Setting IP address...
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Installation and initial startup Process Procedure 1. In Firmware Wizard, cleck <Reset LAN Settings...>. 2. The window <Device connection settings> opens. Enter serial number of the device into the text field <Target device serial num- ber> 3. Select option <Obtain an IP address automatically> 4.
If the device is part of an HPLC system, you should also note the following: The network connection to the router is established The chromatography software has been installed by KNAUER or a com pany authorized by KNAUER. The capillaries have been connected.
Operation 5.1.2 Isocratic operating mode Analysis without gradients The solvent composition is constant during the analysis. The solvent can be recycled. 5.1.3 Optimizing HPLC separations To make your HPLC separations as efficient as possible, pay close atten- tion to the following: Task Explanation...
Operation The pump has been switched on and is in „flow mode“. Tool Syringe with Luer lock Use the purging solvent to be used in the following application. Note: The purging process may take a while during initial operation be- cause the solvent tubes are filled with liquid for the first time.
Operation Solvent in the pump Piston backflushing Reverse phase solvents 50% isopropanol or ethanol with 50 % Water (v/v). Normal phase solvents 100 % isopropanol Buffers with high salt Rinse with water containing concentrations 5% ethanol or isopropanol Legend Outlet from the pump head to the solvent bottle Inlet from the solvent...
Operation Legend Status LED Status Play/Stop Remote control Gradient modus Parameters/Values RFID recognition of the pump head Keypad Fig. 14: LC display and keypad 5.4.1 LED status The status of the device is indicated by an LED on the front side. The co- lor of the LED shows the current status.
Operation 5.4.3 Operating with the keypad The keypad consists of 15 buttons, which allow to operate the device. Function Explanation Numeric pad Activation of numeric pad by pressing the confirmation button at input fields. Changing values. Navigation button Navigation through ...
Operation Menu structure 5.5.1 Main menu The main menu contains the current parameters of the pump. Process Figure 1. To navigate between the different main menus, use the horizontal arrow keys 2. In the four main screens the status of the pump is shown.
Operation 5.5.3 Device setting parameters Menu Explanation Figure Cntl. Interface Setting for external communi- cation connection of the pump. The connections are on the rear side of the device. RS232-11520, RS232-38400, RS232-19200, RS232-9600 serial interface LAN-DHCP: Automatic net- work configuration LAN manual: Manual net- ...
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Operation Solvent factor Data entry for the compressibili- ty of the selected solvent: Water: 0.46 Benzene: 0.95 Chloroform: 0.97 Ethanol: 1.10 Acetone: 1.23 Methanol: 1.21 n-heptane: 1.40 n-hexane: 1.50 Diethyl ether: 1.84 ...
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Operation Analog output Output of analog pump signals setup to external devices by means of the remote terminal strip: Offset data entry for the ana- log output. Full Scale Voltage range opti- ons: 1 V, 2 V, 5 V Signal source ...
Operation — Flow rate value can be changed. This creates a second calibration point which modifies the scaling. Time constant: Set the time constant for the analog input to be used for signal smoot- hing (0.1-10.0 S). The greater the value of the selected time constant, the stronger the applied signal smoothing.
Operation 5.7.1 Create program The use of the display is demonstrated using the example of a program to be created: Program 01 is used to create a solvent gradient (channels A and B) with constant flow of 1 ml/min. The percentage of solvent A is to be continu- ously decreased from 100% to 0 % within 0.50 minutes.
Operation 5.7.3 Change program When new values for the different program lines are entered, the old values are overwritten. Procedure 1. Select the menu Program . 2. Select a program number. 3. Navigate to the desired line and value. 4. Activate the numeric pad by pressing and enter a new value.
Operation Link menu The Link menu displays the status of active links. A maximum of ten pro- gram links between defined programs can be created and saved. Navigate Link menu Procedure 1. Open the desired link number. 2. Use the horizontal arrow keys to navigate through the two lines of the display.
Operation 5.8.2 Run link After the link has been completed, the display switches to the end mode. Procedure 1. Go to the main menu. 2. Navigate to Link/Program in the lower left display line and choose Link with the vertical arrow key 3.
Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge. In case of a request, the technical support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation.
Troubleshooting 7. Troubleshooting First measures: Check all cables and fittings. Check if air has gotten into the supply lines. Check the device for leakages. Further measures: Compare occurring errors with the list of possible errors (see below). ...
Troubleshooting Possible problems and solutions Issue Solution Device cannot be Inspect the power cable to ensure that it is plug- switched on ged into the power supply. When purging, the Check if the venting screw on the pressure sen- pump switches off. sor is opened.
Troubleshooting System messages If other system messages are displayed on the display besides those listed below, please turn the device off and then on. Inform the technical support in case the system message repeats. The system messages are in alphabetical order: System message Auto pump head Switch the device off and on...
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Troubleshooting System message Error input Device error, change device settings. activated GUI communication Switch the device off and on. Inform the techni- failed (internal)! cal support in case the system message repeats itself. Insufficient access Change the entry. Invalid command Change the entry ...
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Troubleshooting System message Not enough space Check the pump to store pro gram Check the number of program lines – a maxi- mum of 100 program lines are possible Not supported Change the entry. Prg not compatible Modify the program or replace the pump head. with pump head! Program does not Create and edit a program.
Customer Support. Maintenance contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a sepa rate maintenance contract: Opening the device or removing housing parts ...
Maintenance and care Material & size Pump head inlet Pump head outlet Stainless steel 10 ml, 7.5 Nm 7.5 Nm 50 ml Inline filter fittings Torque Stainless steel fittings 7.5 Nm PEEK fittings 3.5 Nm Capillary screw fittings Torque Stainless-steel fittings 5 Nm PEEK fittings 1 Nm Flushing the pump Generally, the pump and all its components should be flushed after each operation.
Maintenance and care Maintaining the pump head 8.5.1 Dismounting the pump head Prerequisites The pump head has been purged. The tubes at the inlet and outlet have been removed. The pump head‘s inlet and outlet have been sealed with cap fittings. ...
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Maintenance and care Procedure Process Figure 1. Loosen the finger-tight fitting . Remove the tubing 2. Fixate the outlet fitting with an open-end wrench (size 13). 3. Loosen the fitting with an open-end wrench (size 1/4“). Remove the capillary. Fig. 17: Outlet screw fitting 4.
Maintenance and care 8.5.2 Check valves Clogged check valves do not open and close properly. They cause pres- sure fluctuations and irregular flow. If it is impossible to clean the check valves, replace the whole unit. Procedure Removing the check valves ...
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Maintenance and care 8.5.2.3 Installing the check valve Component defect Damage to components due to excessive tightening possible. Observe the torque of the screw connection Use 5 Nm torque for stainless steel fi ttings. Use 1 Nm torque for PEEK fi ttings. Insert the check valves in the direction of the flow.
Maintenance and care Maintaining the filter cartridge at pressure sensor Clogged inline filters inside of the pressure sensor can cause pressure fluctuations and irregular flow. Inline filters are not cleaned but exchan- ged as an assembly. Note: It is recommended to replace the filter cartridge after 5 000 working hours.
Maintenance and care Procedure Process Figure 4. Loosen the screw connection of the filter cartridge with the open-end wrench (SW 13) and unscrew it by hand. Fig. 24: Loosen screw connection 5. Remove the polluted fil- ter cartridge with the tweezers.
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Maintenance and care Procedure Process Figure 1. Insert the filter cart search into the pressure sensor with the marking facing down (A96601) or with the titanium filter disk facing up (A9661). Use the tweezers for this. Fig. 28: Inserting the filter cartridge A96601 2.
Transporting the device Carefully prepare the device for transport or storage. If you want to return your device to KNAUER for repairs, enclose the Service Request Form which can be downloaded from our website.
Transport and storage algae formation, do not use pure water. Close all inlets and outlets with blind screwings. Prerequisites The pump has been flushed. The pump has been switched off and disconnected from the power supply. Tool Open-end wrench, size 10 ...
160214. 10.2 WEEE registration number KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number classifies to category 8 and 9, which, among others, comprises laboratory equipment.
Technical data 11. Technical data 11.1 Solvent delivery Pump type Analytical high pressure pump Delivery system Dual piston pump with one working pis- ton, one auxiliary Pulsation compensation Active pulsation compensation Flow properties 10 ml pump head: 0.001–10 ml/min 50 ml pump head: 0.01–50 ml/min ...
Technical data 11.2 Communication Analog inputs Flow (0–10 V) Analog control input Flow rate Control RS-232 Remote control connections (Stop, Flow, Pressure, Error) Keypad Programming 19 programs, 10 program links, 1 WAKE UP pro gram (program 20) 11.3 Technical parameters Special features The system pump head is detected automatically using radio frequency...
The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER. The data bases on a literature research on the manufacturer specifications of the materials. The wetted materials of this device are listed in the chapter “Technical data”.
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Chemical compatibility of wetted materials Polyimide (Vespel®) This material is wear-resistant and permanent resilient thermically (up to 200 °C) as well as mechanically. It is chemically broadly inert (pH range 1-10) and is especially resistant against acidic to neutral and organic solvents, but vulnerable to pH strong chemical or oxidizing environments: It is incompatible with concentrated mineral acids (such as sulfuric acid), glacial acetic acid, DMSO and THF.
Chemical compatibility of wetted materials Polychlortrifluorethylene (PCTFE, Kel-F®) The semi-crystalline thermoplastic material is plasticizer-free and dimen- sionally stable, even in a wide temperature range (−240 °C to+205 °C). It is moderately resistent against ether, halogenated solvents and toluene. Halogenated solvents over +60 °C and chlorine gas should not be used. Fluorinated rubber (FKM) The elastomer consisting of fluorinated hydrocarbon stands out due to a high resistance against mineral oils, synthetic hydraulic fluids, fuels,...
Chemical compatibility of wetted materials Mineral wool This insulating material consists of glass or stone wool fibres and isolates in high oxidizing conditions and at high temperatures. Mineral wool is valid as commonly inert against organic solvents and acids. Glass, glass fibre, quartz, quartz glass These mineral materials are resistant against corrosion and wear and are mostly chemical inert.
13. Repeat orders 13.1 Devices Article Order no. Basic device with stainless steel pressure sensor, APC40 without pump head, with accessories Basic device with metal-free pressure sensor, APC60 without pump head, with accessories Pump with 10 ml-Pump head, stainless steel, APC40EA with accessories Pump with 10 ml-Pump head, ceramic, APC60EB...
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Repeat orders Article Order no. 50 ml pump head, stainless steel, accessories AHC20 50 ml pump head, stainless steel, for normal AHC20BA phase applications, accessories 50 ml pump head, stainless steel, for water AHC20FA applications 50 ml pump head, stainless steel for high tempe- AHC20CA rature applications 50 ml pump head, stainless steel, for high tempe-...
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