In-situ Aqua TROLL 600 Operator's Manual
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In-situ Aqua TROLL 600 Operator's Manual

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Aqua TROLL 600
Operator's Manual
Information subject to change without notice. In-Situ, In-Situ logo, Baro Merge, BaroTROLL, HERMIT, HydroVu™, iSitu,
Pocket-Situ, RDO, RuggedCable, RuggedReader, SmarTROLL™, TROLL, VuSitu, and Win-Situ are trademarks or registered
trademarks of In-Situ Inc.
2016. All rights reserved. This product may be covered by patents identified at www.in-situ.com/
©
patents
2021-02-18 | 0096402

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Questions and answers

Chinnathambi Velu
June 5, 2025

How is the SAT% calculated using dissolved oxygen?

0
1 comments:
Mr. Anderson
June 5, 2025

The Aqua TROLL 600 uses an RDO (Rugged Dissolved Oxygen) sensor to measure dissolved oxygen (DO) in mg/L, and from this, it calculates % saturation (SAT%). The % saturation represents the ratio of measured DO to the oxygen saturation level at given temperature and pressure conditions. The device uses EPA-approved In-Situ Methods and includes temperature and pressure compensation to determine accurate % saturation.

This answer is automatically generated

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Summary of Contents for In-situ Aqua TROLL 600

  • Page 1 Aqua TROLL 600 Operator’s Manual Information subject to change without notice. In-Situ, In-Situ logo, Baro Merge, BaroTROLL, HERMIT, HydroVu™, iSitu, Pocket-Situ, RDO, RuggedCable, RuggedReader, SmarTROLL™, TROLL, VuSitu, and Win-Situ are trademarks or registered trademarks of In-Situ Inc. 2016. All rights reserved. This product may be covered by patents identified at www.in-situ.com/ ©...

  • Page 2: Table Of Contents

    Using the RDO Sensor and RDO Fast Cap ..........................30 Ion-Selective Electrode Sensor (ISE) Requirements ......................31 Ammonium Sensor Requirements ............................31 Chloride, Nitrate, and pH/ORP Sensor Requirements .....................31 SDI-12 3-Wire .....................................32 Modbus Master ....................................33 Modbus Master with RS232 (Converter Required) ......................34 1-970-498-1500 www.in-situ.com...
  • Page 3 Zeroing the Depth Sensor ................................50 Software ......................................52 VuSitu Mobile App ..................................52 VuSitu Overview ...................................52 VuSitu Menu Options .................................53 Selecting with Long-press and Swipe ..........................53 VuSitu Data ......................................54 Downloading Your Data ................................54 Sharing Data ....................................54 Viewing Data on a Mac or PC ..............................54 1-970-498-1500 www.in-situ.com...
  • Page 4 Cleaning and Storing the Conductivity Sensor .........................64 Cleaning and Storing the Turbidity Sensor .........................65 Declaration of Conformity ................................67 Appendix ......................................68 Appendix A: Parameter Discovery ............................68 Appendix B: Parameter Numbers and Locations ......................69 Appendix C: Unit IDs ...................................71 Appendix D: Register Data Formats ............................74 1-970-498-1500 www.in-situ.com...

  • Page 5: Introduction

    RDO Sensor Cap are replaceable. It is optional to include a motorized sensor wiper on the instrument. The Aqua TROLL 600 can connect wirelessly via Bluetooth to the VuSitu mobile app or can connect to Win- Situ 5 Software using a cable.

  • Page 6: Obtaining Repair Service

    If you suspect your system is malfunctioning and repair is needed, you can help assure efficient servicing by following these guidelines: 1 Call or email In-Situ Technical Support. Have the product model and serial number available. 2 Be prepared to describe the problem, including how the product was used and the conditions noted at the time of the malfunction.

  • Page 7: Guidelines For Cleaning Returned Equipment

    Representatives that the equipment presents a potential health or biological hazard, we reserve the right to withhold service until proper certification is obtained. Decontamination & Cleaning Statement Company Name Phone Address City State Instrument Type Serial Number Contaminant(s) if known) Decontamination procedure(s) used Cleaning verified by Title Date 1-970-498-1500 www.in-situ.com...

  • Page 8: Safety

    Safety Read the safety information on this page before deploying or configuring your Aqua TROLL 600. If you have questions, contact In-Situ Technical Support for assistance. • Use D-cell alkaline batteries. • Do not use batteries of different ages or types.

  • Page 9: General Specifications

    Measurement: 40 mA typical, 75 mA Max Win-Situ 5 Software, VuSitu Mobile App on select Interface mobile devices using Android 4.4 with Bluetooth 2.0 Cable Vented or non-vented polyurethane or vented Tefzel® Hex screw driver 0.050 in. (1.3 mm) 1-970-498-1500 www.in-situ.com...

  • Page 10: External Power Source Requirements

    2 year - sonde, sensors (excluding pH/ORP); 1 year - pH/ORP sensor Warranty Other - see warranty policy at www.in-situ.com Specifications are subject to change without notice. Android is a trademark of Google, Inc. Bluetooth is a trademark of Bluetooth SIG, Inc.
  • Page 11 60.2 cm (23.7 in) Diameter 47 mm (1.85 in) Instrument Dimensions with Restrictor Off Total length with wiper brush on 60.2 cm (23.7 in) Total length with wiper brush off 47 mm (1.85 in) Diameter 41.66 mm (1.64 in) 1-970-498-1500 www.in-situ.com...

  • Page 12: Sensor Specifications

    2 years or User calibration Rhodamine - 5 to 50° C greater only if needed * Shelf life is included in total life of sensor. ** Field life and calibration frequency dependent on site conditions. 1-970-498-1500 www.in-situ.com...

  • Page 13: Potential Interferents

    • Do not use in organic solvents (e.g., acetone, chloroform, methylene chloride, etc.), which may destroy the sensing element Conductivity/Temperature Sensor Soaking the Conductivity/Temperature sensor in vinegar for longer than one hour can cause serious damage. Ammonium, Chloride, and Nitrate Interferent Concentrations 1-970-498-1500 www.in-situ.com...
  • Page 14 The table below lists concentrations of possible interfering ions that cause 10% error at various levels (in ppm) of N03-. 100 ppm NH4+ 10 ppm NH4+ 1 ppm NH4+ Perchlorate (CIO 7 x 10-2 7 x 10-3 7 x 10-4 Iodide (I 0.04 Chlorate (CIO Cyanide (CN Bromide (Br Nitrite (NO Hydrogen Sulfide (HS 1-970-498-1500 www.in-situ.com...
  • Page 15 Total dissolved solids: ppt, ppm Resistivity: Ohms- Density: g/cm3 Methodology Std. Methods 2510, EPA 120.1 *Accuracy at calibration points. For greatest accuracy over instrument life, keep the conductivity cell submersed in water for 24-48 hours prior to calibration and deployment. 1-970-498-1500 www.in-situ.com...
  • Page 16 Sensor Type Removable with replaceable RDO-X Cap Response Time RDO-X Cap: T63<15s, T90<45s, T95<60s Units of Measure mg/L, % saturation, ppm EPA-approved In-Situ Methods (under the Alternate Methodology Test Procedure process): 1002-8-2009, 1003-8-2009, 1004-8-2009 Level, Depth, Pressure Sensor Specifications Accuracy Typical ±0.1% full scale (FS)
  • Page 17 Units of Measure pH units Methodology Std. Methods 4500-H+, EPA 150.2 *At thermal equilibrium. Temperature Sensor Specifications Accuracy ±0.1° C Range -5 to 50° C (23 to 122° F) Resolution 0.01° C Sensor Type Replaceable Response Time** T63<2s, T90<15s, T95<30s 1-970-498-1500 www.in-situ.com...
  • Page 18 25 m, 30 PSI Range 0-10,000 mg/L as N Resolution 0.01 mg/L Sensor Type Removable Response Time* T90 < 10sec, T95 < 30sec Units of Measure mg/L, ppm, mV Methodology Std. Methods 4500-NH D, EPA 350.3 *Between calibration points. 1-970-498-1500 www.in-situ.com...
  • Page 19 T90 < 10sec, T95 < 30sec Units of Measure mg/L, ppm, mV Methodology Std. Methods 4500-NO3D * Between calibration points. Chlorophyll a Sensor Specifications R2 > 0.999 for serial dilutions of 0-1000 µg/L Chl a in Linearity MeOH Max Depth 200 m 1-970-498-1500 www.in-situ.com...
  • Page 20 BGA-PE Sensor Specifications R2 > 0.999 for serial dilutions of PE standards from Linearity 0-1000 µg/L PE Max Depth 200 m 0-100 RFU Range 0-1000 µg/L PE .001 RFU Resolution .01 µg/L PE Sensor Type Removable Response Time* T63<1s, T90<1s, T95<1s 1-970-498-1500 www.in-situ.com...
  • Page 21 200 m 0-100 RFU Range 0-1000 µg/L .001 RFU Resolution .01 µg/ Sensor Type Removable Response Time* T63<1s, T90<1s, T95<1s Concentration: µg/L Units of Measure Fluorescence: RFU Excitation Wavelength (nominal) 530 nm Detection Wavelength 580 nm to 660 nm 1-970-498-1500 www.in-situ.com...

  • Page 22: Instrument Overview

    The built-in LCD screen displays battery status, connectivity info, and other vital information. Setup is easy with the VuSitu app and a Bluetooth-enabled mobile device. The Aqua TROLL 600 can record data to an internal micro SD card and works with telemetry devices for remote monitoring.

  • Page 23: System Components

    Cable Extender 0051490 Large Desiccant (titanium connector) 0051810 Large Desiccant (ABS connector) 0053550 Small Desiccant (3 pack) - storage desiccant 0052230 Desiccant Refill Kit for Large or Outboard Desiccant 0029140 Calibration and Maintenance RDO Classic Cap Replacement Kit 0079790 1-970-498-1500 www.in-situ.com...
  • Page 24 140 ppm, 1400 ppm, DI water) Low-Flow Complete Low-Flow Kit (includes flow cell with 0079790 fittings and stake, base plate, documentation) Flow Cell Kit 0078990 Flow Cell Base Plate 0079810 Fittings Kit 0032130 Pelican Case with Foam Insert 0066860 1-970-498-1500 www.in-situ.com...
  • Page 25 SDI-12, analog (4-20 mA), or Modbus communication protocol. RuggedCable System with female to female connectors Stripped-and-tinned RuggedCable System with female connector Stripped-and-tinned RuggedCable System with male connector (short length that converts a cable with a twist-lock connector to a stripped- and-tinned cable) 1-970-498-1500 www.in-situ.com...

  • Page 26: Getting Started

    Aqua TROLL 600 Quickstart Guide Box Contents 1 Documentation and software 2 Wiper motor and brush/wiper 3 Aqua TROLL 600 sonde 4 Water quality sensors (2 of 4) 5 Water quality sensors (2 of 4) 6 RDO sensor cap 7 Accessory supplies 8 pH/ORP sensor maintenance supplies...
  • Page 27 Apply vacuum flat edge on instrument instrument in other. Push grease to O-ring. connector. and twist until click. When using a conductivity sensor and turbidity sensor together, install them side-by-side to maximize performance. 1-970-498-1500 www.in-situ.com...
  • Page 28 Connect. devices. An iOS device To connect to another automatically connects instrument, press Disconnect and then to the closest In-Situ instrument. Choose or Add Device. VuSitu displays a list of available connections. VuSitu displays the Tap the serial number Connected Instrument...
  • Page 29 Learn more. The product manual provides complete instructions about calibration, configuration, and deployment. See the Frequently Asked Questions sections of the In-Situ website for online help: https://in-situ.com/us/faq. 1-970-498-1500 www.in-situ.com...

  • Page 30: Handling Ph And Ion-Selective Electrode Sensors (Ises)

    The wiper can severely reduce the life of the RDO Fast Cap. Wear will vary by application. Verify sensor performance prior to use and replace the Fast Cap if damaged. Inspect foil prior to each use. If damaged, replace cap. RDO Fast Cap Wiper 1-970-498-1500 www.in-situ.com...

  • Page 31: Ion-Selective Electrode Sensor (Ise) Requirements

    ISE sensors require one or more additional sensors to function. See the details below and install the required sensors to use an ISE. Ammonium Sensor Requirements Conductivity/ Temperature Sensor Temperature Sensor To calculate ammonia... Conductivity/ pH Sensor Temperature Sensor Chloride, Nitrate, and pH/ORP Sensor Requirements Conductivity/ Temperature Sensor Temperature Sensor 1-970-498-1500 www.in-situ.com...

  • Page 32: Sdi-12 3-Wire

    SDI-12 3-Wire 1-970-498-1500 www.in-situ.com...

  • Page 33: Modbus Master

    Modbus Master 1-970-498-1500 www.in-situ.com...

  • Page 34: Modbus Master With Rs232 (Converter Required)

    Modbus Master with RS232 (Converter Required) 1-970-498-1500 www.in-situ.com...

  • Page 35: Modbus Plc Interface

    • Firmware version 1.71 or later must be installed on the sonde. For information about the full Modbus capabilities of your sonde, see the Aqua TROLL 500/600 Interface Specification at www.in-situ.com/support/type/documentation. Setting Up Instrument 1. Install the sensors and turn on the display by holding the instrument vertically.
  • Page 36 Parameter Id: The ID of the parameter for this location. See: Full System Specification float Off line sentinel value: The value that’s returned on error or if the parameter isn’t available. The default sentinel is 0.0 1-970-498-1500 www.in-situ.com...
  • Page 37 6. Set the type of register to: 32-bit float a. If asked by the PLC this is 2 registers 7. Set the byte order to: Big Endean (MSB) a. This should be the default and may not be configurable on all PLCs 1-970-498-1500 www.in-situ.com...

  • Page 38: Lcd Screen

    Possible Port Statuses Sensors installed Port plugs installed Sensor/port error Possible Power Statuses Power level NOT within Power level within specs specs Possible Connected Statuses Connected via Bluetooth Connected via cable Error Messages Port(s) empty Cap expiration 1-970-498-1500 www.in-situ.com...

  • Page 39: Starting A Log With The Lcd Menu

    Activate the LCD menu and select Data Log. Choose New. Stopping a Log Set the logging interval. The log starts immediately. You can’t update instrument firmware while a log is running. Stop a log to update the sonde or sensors. 1-970-498-1500 www.in-situ.com...

  • Page 40: Stopping A Log

    Activate the LCD menu and scroll to Display. Tap Select Contrast. once to select. Tilt the sonde to the left to decrease the contrast. Or, tilt the sonde to the right to increase contrast. Tap the Aqua TROLL 600 logo to set the contrast. 1-970-498-1500 www.in-situ.com...

  • Page 41: Setting Or Changing Menu Language

    Select Yes if an update is available. If an update is present on the SD card, the screen will indicate the previous firmware version, a right-facing arrow, and the new version of the firmware. 1-970-498-1500 www.in-situ.com...

  • Page 42: Micro Sd Card

    Micro SD Card The Aqua TROLL 600 uses a micro SD card for data storage and updating sonde firmware. You can remove the SD card and replace it with another for data download, or use the same card. An SD card is not required to log data.
  • Page 43 11 Scroll to Yes by tilting the instrument. Tap the Aqua TROLL 600 logo once. 12 The LCD screen will display icons while updating. Once the update is complete the Aqua TROLL 600 and LCD screen will restart.

  • Page 44: Replacing Desiccant

    Use the solution-based procedure described below to calibrate all sensors except RDO. You will need the following items. • Calibration standard, or multiple standards for multi-point calibrations • Wireless TROLL Com connected to the Aqua TROLL 600 • Bluetooth-enabled mobile device Hold the sonde upright to activate...

  • Page 45: Turbidity Sensor Calibration

    Select Set User Defined to begin calibrating with the new value. If you are using an In-Situ standard and the app does not automatically detect it, perform the sensor cleaning and maintenance procedure, then select Retry Auto Detect.

  • Page 46: Rdo 100% Saturation Calibration: Water Saturated Air

    RDO 100% Saturation Calibration: Water Saturated Air Use the procedure below to calibrate the Aqua TROLL 600 RDO sensor, or see the next section for an alternative method. Sponge Place the restrictor in calibration Saturate a small sponge with Place sponge in restrictor.

  • Page 47: Rdo Salinity Setting

    From VuSitu's main Select Instrument From the Instrument Enter your desired menu, select Connected Settings from the menu Settings menu, select salinity compensation Instrument. Salinity Setting. setting and press Save. at the bottom of the screen. 1-970-498-1500 www.in-situ.com...
  • Page 48 Calibration Frequency Recommendations In-Situ sensors are factory calibrated across the entire range of each sensor, and thus achieve a very high degree of accuracy and stability for extended periods of time without user calibration. In-Situ recommends inserting the instrument into a known calibration standard to check the accuracy of a sensor prior to performing any user calibration if you suspect drift, unless a user calibration is required by a standard operating procedure.
  • Page 49 Factory calibration includes a thorough cleaning, full functionality check and sensor adjustments to all applicable sensors over the entire calibrated temperature range. We recommend a factory calibration every 12 months or when the unit appears to drift significantly. 1-970-498-1500 www.in-situ.com...

  • Page 50: Zeroing The Depth Sensor

    Zeroing the Depth Sensor The Aqua TROLL 600 uses its pressure reading and specific gravity value to calculate depth. The pressure sensor is located at the center of the instrument, but depth is reported at the smart sensor faces. An embedded gyroscope compensates for the distance between these sensors and allows the sonde to be deployed in any orientation (vertical, horizontal, angled).
  • Page 51 Sensor Office (OL) and no longer Remove two sensors and reinstall in communicating with the instrument separate port. 3.) Call (800-446-7488) or contact In-Situ Technical Support (support@in-situ.com). 1.) Inspect wiper to ensure surface is free of debris and the wiper is spinning correctly.

  • Page 52: Software

    Download VuSitu from the Google Play Store or the Apple App Store. VuSitu Overview VuSitu is the mobile user interface and control application for In-Situ water quality instruments. The app allows you to accomplish the following tasks. • View live readings that update every 10 seconds •...

  • Page 53: Vusitu Menu Options

    Selecting with Long-press and Swipe Press and hold any item Press and swipe left to Press and swipe right to in a list of files. You can reveal the delete and reveal the sharing icon. now select multiple files. share icons. 1-970-498-1500 www.in-situ.com...

  • Page 54: Vusitu Data

    Viewing Data on a Mac or PC You’ll need to extract your files to view them. To do that on a Mac, double-click the Zip folder. On a PC, right-click on the folder and choose Extract. Then open your files in Excel. 1-970-498-1500 www.in-situ.com...

  • Page 55: Live Readings In Vusitu

    Live Readings Mode Take a single reading Take readings at two- and save to Snapshot second intervals. file. View Snapshot file from View file from Menu > Menu > Data Files. Data Files. Check Snapshot option. Check Live option. 1-970-498-1500 www.in-situ.com...

  • Page 56: Exporting Data Files

    Data view and export. file and choose how you Files screen. wish to share it. Viewing Data Files Open a VuSitu data file in any web browser. Click the button at the top left to generate a CSV. 1-970-498-1500 www.in-situ.com...

  • Page 57: Vusitu Locations

    As an alternative, you can manually enter latitude and longitude values and tap Apply. Or, tap and hold a specific point on the map to drop a pin there. 1-970-498-1500 www.in-situ.com...
  • Page 58 2 The active Location is marked with a green check mark. If no Location has been selected data will be associated with the Default Location. 3 Tap the desired location in the list. 4 The Live Readings screen appears with the site selected. 1-970-498-1500 www.in-situ.com...
  • Page 59 3 Tap the Overflow Menu in the upper-right portion of the screen. 4 Select Edit Location to make changes, or Archive Location to remove it from the list. Archived Locations can be restored at any time by tapping the Location, accessing the Overflow Menu and tapping Restore Location. 1-970-498-1500 www.in-situ.com...

  • Page 60: Care And Maintenance

    Dampen the sponge Replace the caps at both Thread the restrictor wiper. inside the pH sensor ends of the sensor. Use onto the sonde. storage cap with electrical tape to seal the Storage Solution or pH storage cap. calibration standard. 1-970-498-1500 www.in-situ.com...

  • Page 61: Cleaning The Sonde

    If the sensor fails to calibrate after you replace the fill solution, replace the reference junction. Replacing the Filling Solution Remove sensor from Discard old solution. Insert tube from filling Squeeze solution into sonde and unscrew solution bottle into reservoir until full. Slowly reference junction. sensor. remove tube. 1-970-498-1500 www.in-situ.com...
  • Page 62 1 Clean the sensor with warm water and mild soap. 2 Soak the sensor in 0.1 M HCl solution for 10 minutes and then rinse with deionized water. After cleaning, rinse the sensor with water, and then soak overnight in pH 4 buffer. 1-970-498-1500 www.in-situ.com...

  • Page 63: Cleaning And Storing The Rdo Sensor

    4 If extensive fouling or mineral buildup is present, soak the sensor in vinegar for 15 minutes, then soak in deionized water for 15 minutes. Do not use organic solvents—they will damage the sensor cap. Do not remove the sensor cap when rinsing or brushing. 5 After cleaning the sensor, perform a 2-point calibration. 1-970-498-1500 www.in-situ.com...

  • Page 64: Cleaning And Storing The Conductivity Sensor

    • Using a soft brush, gently clean the sensor pins and temperature button. Ensure removal of all material/ film around the base of the pins and temperature button. • Soak the sensor in 0.10% HCl for 10 minutes and then rinse thoroughly with distilled water. 1-970-498-1500 www.in-situ.com...

  • Page 65: Cleaning And Storing The Turbidity Sensor

    Replacing Wiper Bristles Wiper bristles need to be replaced based on site conditions. In-Situ recommends replacing bristles at least every 12 months or when visibly bent, damaged, or fouled.
  • Page 66 Cleaning the copper restores the effectiveness of the restrictor’s antifouling capability. 1 Remove the restrictor from the sonde. 2 Remove the restrictor end cap. 3 Submerge the restrictor in white vinegar (acetic acid) for 24 hours. 4 Rinse the restrictor in water and air dry. 1-970-498-1500 www.in-situ.com...

  • Page 67: Declaration Of Conformity

    Declaration of Conformity 1-970-498-1500 www.in-situ.com...

  • Page 68: Appendix

    Appendix B for a description of parameter IDs. Parameter ID Map Register 13...2 6984 14...3 6985 30...19 6986 46...35 6987 62...51 6988 78...67 6989 94...83 6990 110...99 6991 126.115 6992 142...131 6993 158...147 6994 174...163 6995 190...179 6996 206...195 6997 219...211 1-970-498-1500 www.in-situ.com...

  • Page 69: Appendix B: Parameter Numbers And Locations

    Dissolved Oxygen 5590 177 = % Saturation % Saturation Chloride (Cl 5612 5611 117 = mg/L Turbidity 5619 5618 194 = NTU Oxygen Partial 5654 5653 26 = torr Pressure Total Suspended 5661 5660 117 = mg/L Solids 1-970-498-1500 www.in-situ.com...
  • Page 70 257 = RFU Intensity Blue Green Algae-Phycocyanin 5822 5821 118 = µg/L Concentration Blue Green Algae-Phycocyanin 5829 5828 257 = RFU Fluorescence Intensity Blue Green Algae- 5850 5849 118 = µg/L Phycocerythrin Concentration 5857 5856 257 = RFU 1-970-498-1500 www.in-situ.com...

  • Page 71: Appendix C: Unit Ids

    Millimeters of Mercury (0 to C) inHg Inches of Mercury (4 to C) Centimeters of water (4 to C) Inches of water (4 to C) Torr Torr standard atmosphere Distance/Length (33-48) millimeters Centimeters Meters Kilometer Inches Feet Coordinates (49-64) Degrees Minutes Seconds 1-970-498-1500 www.in-situ.com...
  • Page 72 Density (129-144) g/cm3 Grams per cubic centimeter pH (145-160) Voltage (161-176) µV micro Volts milli Volts Volts Dissolved Oxygen (DO) % Saturation (177-192) % sat Percent saturation Turbidity (193-208) formazin nephelometric units nephelometric turbidity units formazin turbidity units 1-970-498-1500 www.in-situ.com...
  • Page 73 Available (was Cubic meters per day) Liters per second ML/day Millions of liters per day mL/min milliliters per minute kL/day Thousands of liters per day Volume (225-240) Cubic feet Gallons Mgal Millions of gallons Cubic meters Liters acre-ft Acre feet 1-970-498-1500 www.in-situ.com...

  • Page 74: Appendix D: Register Data Formats

    Appendix D: Register Data Formats The Modbus protocol specification requires any multiple-byte data type to be transmitted in Big Endean order, or most significant byte (MSB) first. In-Situ devices shall use the following register data formats. Register Data Type Table...

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