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Summary of Contents for ThermoFisher Scientific TX-750
- Page 1 Thermo Scientific TX-750 Instruction Manual 50119938-e • 07 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
- Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...
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Page 5: Table Of Contents
Cleaning Intervals ....................6-2 Cleaning ........................6-2 Disinfection ......................6-3 Decontamination ..................... 6-4 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-6 RCF-Values .......................A-1 Rectangular buckets 75003614 ................A-1 Round bucket 75003608 ..................A-2 Chemical Compatibility Chart ................B-1 Thermo Scientific 75003607 TX-750... - Page 6 Contents 75003607 TX-750 Thermo Scientific...
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Page 7: Preface
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative. Precautions In order to ensure safe operation of the TX-750, the following general safety regulations must be followed: • Do not remove the magnet at the rotor bottom •... - Page 8 ATTENTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. 75003607 TX-750 Thermo Scientific...
- Page 9 Rotor Specifications Contents • “Thechnical Data” page 1-2 Thermo Scientific 75003607 TX-750...
- Page 10 19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 50 / 55 50 / 55 50 / 55 Perosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003607 TX-750 Thermo Scientific...
- Page 11 19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 40 / 50 40 / 50 40 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750...
- Page 12 19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 75 / 60 75 / 60 75 / 60 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003607 TX-750 Thermo Scientific...
- Page 13 19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 65 / 50 65 / 50 65 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750...
- Page 14 19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 50 / 55 50 / 55 50 / 55 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003607 TX-750 Thermo Scientific...
- Page 15 19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 40 / 50 40 / 50 40 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750...
- Page 16 19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 75 / 60 75 / 60 75 / 60 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003607 TX-750 Thermo Scientific...
- Page 17 19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 65 / 50 65 / 50 65 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750...
- Page 18 19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 80 / 60 80 / 60 80 / 60 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-10 75003607 TX-750 Thermo Scientific...
- Page 19 19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 65 / 50 65 / 50 65 / 50 Aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-11 Thermo Scientific 75003607 TX-750...
- Page 20 Max. / min. radius [cm] 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 50 / 55 50 / 55 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-12 75003607 TX-750 Thermo Scientific...
- Page 21 Max. / min. radius [cm] 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 40 / 50 40 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-13 Thermo Scientific 75003607 TX-750...
- Page 22 Maximum RCF value at n 4,816 Max. / min. radius [cm] 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 75 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-14 75003607 TX-750 Thermo Scientific...
- Page 23 Maximum RCF value at n 4,031 Max. / min. radius [cm] 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 65 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-15 Thermo Scientific 75003607 TX-750...
- Page 25 Accessories Contents • “Rotor data” page 2-2 “Accessories for round bucket” page 2-2 • • “Rotor data” page 2-4 “Accessories for rectangular bucket” page 2-4 • Thermo Scientific 75003607 TX-750...
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Page 26: Chapter 1 Rotor Specifications
Accessories Thermo Scientific General Purpose Rotors TX-750 Round Buckets TX-750 Swinging Bucket Aluminum Rotor with Round Buckets Rotor Specifications Capacity (ml) 4 x 750 Tube Dimensions (mm) 98 x 133 Angle Maximum Speed (rpm) 4.700 K Factor 9783 Net Weight (kg) -
Page 27: Chapter 2 Accessories
RIA Tubes 13x75 75003724 RIA Decanting Tubes 13x75 75003732 1.5/2 Conical Microtube 11x42 75003733 T-75 Nunc Easy 75008383 Flask T-25 Nun Easy Flask 75008384 Standard Microplates 75003617 (up to 20mm) Deepwell Microplates 75003617 (up to 40mm) Thermo Scientific 75003607 TX-750... - Page 28 Accessories Thermo Scientific General Purpose Rotors TX-750 Rectangular Buckets TX-750 Swinging Bucket Aluminum Rotor with Rectangular Buckets Rotor Specifications Capacity (ml) 4 x 250 Tube Dimensions (mm) 63 x 130 Angle Maximum Speed (rpm) K Factor 8,983 Net Weight (kg)
- Page 29 Accessories Thermo Scientific General Purpose Rotors TX-750 Rectangular Buckets (cont'd) Tube Ordering Information Sealing Assembly Required: Adapters Required: Accessories Required: Tube Vol. Max. Dims ØxL Cat. No. Vol. Fill Description Qty. Speed No. of (mm) Cat. No. Description Cat. No.
- Page 30 Accessories 75003607 TX-750 Thermo Scientific...
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Page 31: Autolock Tm
AutoLock Contents “Rotor Installation” page 3-2 • • “Removing the Rotor” page 3-3 Thermo Scientific 75003607 TX-750... -
Page 32: Rotor Installation
WARNING If the rotor cannot be properly locked in place after several attempts, then the AutoLock is defective and you are not permitted to operate the rotor. Check for any damage to the rotor, damaged rotors must not be used. Keep the hub area clear of objects. 75003607 TX-750 Thermo Scientific... -
Page 33: Removing The Rotor
2. Grab the rotor handle with both hands and press against the green AutoLock button. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle. Make sure not to jam the rotor while doing this. Figure 4. Handling of AutoLock Thermo Scientific 75003607 TX-750... - Page 34 AutoLock Removing the Rotor 75003607 TX-750 Thermo Scientific...
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Page 35: Rotor Loading
Rotor Loading Contents “Before a Run” page 4-2 • • “Proper Loading” page 4-2 “Improper Loading” page 4-3 • • “Maximum Loading” page 4-3 “Cycle Counter” page 4-4 • Thermo Scientific 75003607 TX-750... -
Page 36: Before A Run
To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times. You can use two rectangular buckets or two round buckets or two microplate carriers. Make sure that the opposing receptacles are holding tubes of the same weight. 75003607 TX-750 Thermo Scientific... -
Page 37: Improper Loading
There are two options available for centrifuging samples whose weight (including adaptor) exceeds the maximum permissible load: • Reduce the fill level. Reduce the speed. Use the table or the formula: n max Maximum permissible load n adm --------------------------------------------------------------------- - Effective load = admissible speed = maximum speed Thermo Scientific 75003607 TX-750... -
Page 38: Cycle Counter
Replace the buckets when the specified number of cycles is reached. Service life examples Usage profile Maximum service life at 50,000 cycles frequent use 7 years 20 runs / day 220 days / year 75003607 TX-750 Thermo Scientific... -
Page 39: Aerosol-Tight Applications
Aerosol-tight Applications Contents “Basic Principles” page 5-2 • • “Fill level” page 5-3 “Checking the aerosol Tightness” page 5-2 • Thermo Scientific 75003607 TX-750... -
Page 40: Basic Principles
This releases the carbonic acid gas which is bound in the water, resulting in excess pressure. Do not apply pressure to the lid when doing so! Leaks can be detected by escaping water or the sound of escaping gas. 75003607 TX-750 Thermo Scientific... - Page 41 Bucket with closed lid CAUTION If the latch is not flipped down, the caps could be damaged during centrifuging. If the latch has not clicked into place, the bucket is not aerosol-tight. Never raise the bucket at its latch. Thermo Scientific 75003607 TX-750...
- Page 42 Aerosol-tight Applications Checking the aerosol Tightness 75003607 TX-750 Thermo Scientific...
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Page 43: Maintenance And Care
Maintenance and Care Contents “Cleaning Intervals” page 6-2 • • “Cleaning” page 6-2 “Disinfection” page 6-3 • • “Decontamination” page 6-4 “Autoclaving” page 6-5 • • “Service of Thermo Fisher Scientific” page 6-6 Thermo Scientific 75003607 TX-750... -
Page 44: Cleaning Intervals
• Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. 75003607 TX-750 Thermo Scientific... -
Page 45: Disinfection
CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific 75003607 TX-750... -
Page 46: Decontamination
For general radioactive decontamination, use a solution of equal parts of 70 % ethanol, 10 % SDS and water. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 75003607 TX-750 Thermo Scientific... -
Page 47: Autoclaving
Place the rotor on a flat surface. Note No chemical additives are permitted in the steam. CAUTION Never exceed the permitted temperature and duration when autoclaving. If the rotor shows signs of corrosion or wear, it must be replaced. Thermo Scientific 75003607 TX-750... -
Page 48: Service Of Thermo Fisher Scientific
• the suitability of the set-up site; • the lid lock and the safety system; • the rotor; • the fixation of the rotor and the drive shaft. Thermo Fisher Scientific offers inspection and service contracts for this work. 75003607 TX-750 Thermo Scientific... -
Page 49: Rcf-Values
1600 19.5 1700 19.5 1800 19.5 1900 19.5 2000 19.5 2100 19.5 2200 19.5 1055 2300 19.5 1153 2400 19.5 1256 2500 19.5 1363 2600 19.5 1474 2700 19.5 1589 2800 19.5 1709 2900 19.5 1833 Thermo Scientific 75003607 TX-750... -
Page 50: Round Bucket 75003608
Table A-2.Round bucket 75003608 Speed (rpm) Min. radius Max. radius RCF Rmin RCF Rmax 19.5 19.5 19.5 19.5 19.5 19.5 19.5 1000 19.5 1100 19.5 1200 19.5 1300 19.5 1400 19.5 1500 19.5 1600 19.5 1700 19.5 1800 19.5 75003607 TX-750 Thermo Scientific... - Page 51 3900 19.5 1411 3316 4000 19.5 1485 3488 4100 19.5 1560 3665 4200 19.5 1637 3846 4300 19.5 1716 4031 4400 19.5 1796 4221 4500 19.5 1879 4415 4600 19.5 1964 4613 4700 19.5 2050 4816 Thermo Scientific 75003607 TX-750...
- Page 52 RCF-Values 75003607 TX-750 Thermo Scientific...
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Page 53: Chemical Compatibility Chart
S U U U U U U U - M S M - Boric Acid U S S M S S U S S S S S S S S S U S S S S S S S S S S Thermo Scientific 75003607 TX-750... - Page 54 S S S U S U S S S U ® Ficoll-Hypaque M S S - S S S - S S S S - S S - S S S S S S S M S S S 75003607 TX-750 Thermo Scientific...
- Page 55 S S S U S S M S S S S S U S U M S S S S S S S M S M U Methylene Chloride U U U U M S S U S U U S U U U U U M U U U S S M U S U Thermo Scientific 75003607 TX-750...
- Page 56 S S S S S S S S S S S S S S S S S S S Picric Acid S S U - S M S S S M S U S S S U S S S S U S U M S M S 75003607 TX-750 Thermo Scientific...
- Page 57 S S S S S S S S S S S S S S S S S S S S S S S Citric Acid (10%) M S S M S S M S S S S S S S S S M S S S S S S S S S S Thermo Scientific 75003607 TX-750...
- Page 58 Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. Because no organized chemical resistance data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. 75003607 TX-750 Thermo Scientific...
- Page 59 CLICKSeal ............ 5-3 Cycle Counter ..........4-4 Decontamination .......... 6-4 Disinfection ..........6-3 Fill Level ............5-2 Improper Loading ......... 4-3 Maintenance ..........6-1 Maximum Loading ........4-3 Precautions ........... 1-iii Preface ............1-iii Proper Loading ..........4-2 Thermo Scientific 75003607 TX-750...
- Page 60 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/rotors © 2009-2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.
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