The Amersham Imagequant 800 Instrument - GE Amersham ImageQuant 800 User Manual

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The Amersham ImageQuant 800 instrument

About the Amersham
ImageQuant 800
The Amersham ImageQuant 800 is an imaging instrument designed to detect and
quantify proteins, DNA, and other biological samples, such as bacterial colonies. A cooled
8.3 megapixel CCD camera is used to capture high resolution digital images of elec-
trophoretically separated protein and DNA bands in gels or membranes obtained through
blotting methods. Use of the optional NP lens also allows for chemiluminescence image
capture from multi-well plates and petri dishes. The instrument can capture images of
chemiluminescent, fluorescent, and colorimetric samples, depending on the system
configuration. It is also possible to capture optical densitometry (OD) images of stained
gels to accurately measure amount of proteins in bands on a gel. Image files obtained
from the instrument can be analyzed with standard image analysis software, such as
GE Healthcare ImageQuant analysis software. Contact your GE Healthcare representative
for more information regarding analysis software.
The instrument is designed to be used for research purposes in life science laboratories
within academia and industry.
Instrument configuration
The Amersham ImageQuant 800 is available in several different configurations, which
can be upgraded to improve functionality. Depending on the configuration of your
Amersham ImageQuant 800 instrument, not all of the functions described in this manual
will be available. For more information on Amersham ImageQuant 800 instrument con-
figurations and upgrades, see the Amersham ImageQuant 800 Operating Instructions.
Imaging capabilities
A fully equipped Amersham ImageQuant 800 instrument has the following LED light
sources:
Light source
UV Epi
Blue Epi
Green Epi
Red Epi
Amersham ImageQuant 800 User Manual 29383813 AA
1.2 The Amersham ImageQuant 800 instrument
Emission wavelength
365 nm
460 nm
535 nm
635 nm
1 Introduction
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