Page 1
Translation of the original operating manual 10/2013 Rev. 2.21 of 08/05/2020 Sigma 1-16K;Refrigerated Centrifuge;Sigma 1-16K IVD; Refrigerated Centrifuge part no. 0700302 Sigma 1-16K Sigma 1-16K IVD From serial no. 145758 Refrigerated Centrifuge Operating Manual Please retain for later use!
Page 2
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD Version 10/2013, Rev. 2.21 of 08/05/2020 • sb 2 / 70 Translation of the original operating manual, part no. 0700302...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability......................9 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................10 Layout and mode of operation ..................
Page 6
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD Table of contents Storage and transport ....................... 23 Dimensions and weight ....................23 Storage conditions ......................23 Notes on transport ......................23 Packaging ........................24 Transport safety device ....................24 Set-up and connection ...................... 25 Installation site ........................
Page 7
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD Table of contents Malfunctions and error correction ..................38 General malfunctions ...................... 38 7.1.1 Emergency lid release ....................39 Table of error codes ......................40 Service contact ....................... 41 Maintenance and service ....................42 Maintenance ........................
Page 8
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD Table of contents Version 10/2013, Rev. 2.21 of 08/05/2020 • sb 8 / 70 Translation of the original operating manual, part no. 0700302...
• compliance with the inspection and maintenance instructions. Sigma Laborzentrifugen GmbH cannot be held liable for any damage resulting from non-compliance. → Warranty and liability The warranty and liability are subject to our "General Conditions"...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 1 General information → Copyright The copyright concerning the operating manual remains with Sigma Laborzentrifugen GmbH. The operating manual is solely intended for the operator and their personnel. It includes instructions and information that must not be •...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 2 Layout and mode of operation → Layout and mode of operation → Layout of the centrifuge 2.1.1 Functional and operating elements Display User interface (see chapter 6.3.1 - "User interface") Fig.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 2 Layout and mode of operation 2.1.2 Name plate Manufacturer Power consumption Max. speed Max. kinetic energy Serial number Part number Type Nominal voltage CE mark in compliance with the directive...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 2 Layout and mode of operation → Mode of operation → 2.2.1 Centrifugation principle Centrifugation is a process for the separation of heterogeneous mixtures of substances (suspensions, emulsions, or gas mixtures) into their components.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 2 Layout and mode of operation → 2.2.2.1 Speed, radius, and relative centrifugal force The acceleration g, which the samples are subject to, can be increased by increasing the radius in the rotor chamber and by increasing the speed.
Refrigerated Centrifuge Sigma 1-16K IVD 3 Safety → Safety → Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma: Dangerous voltage On (Power) Hot surface Off (Power) Name plate (see chapter Caution! Risk of bruising 2.1.2 - "Name plate")
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 3 Safety Safety indications on the centrifuge must be kept readable at all times. If necessary, they must be replaced. NOTE Not all of the symbols/labels are used for this centrifuge type.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 3 Safety → Responsibility of the operator The operator undertakes to authorise only trained, specialised personnel to work on the centrifuge (see chapter 3.4 - "Operating personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 3 Safety → Safety instructions 3.6.1 Electrical safety To reduce the risk of electrical shock, the centrifuge uses a three-wire electrical cord and plug to connect the equipment to earth-ground. To preserve this safety feature: •...
Laborzentrifugen GmbH (see chapter 7.3 - "Service contact"). • If the housing becomes damaged, do not use the centrifuge. Contact WARNING the service department of Sigma Laborzentrifugen GmbH (see chapter 7.3 - "Service contact"). • Ensure that all repairs are performed only by authorised and specialised personnel.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 3 Safety → 3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: • Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up and connection").
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 3 Safety 3.6.7 Safety of rotors and accessories 3.6.7.1 Service life The rotors and accessories have a limited service life. Perform regular checks (at least once per month) for safety reasons! •...
(see chapter 7.2 - "Table of error codes"). 3.7.4 Earth conductor check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument. Please contact the Sigma service department (see chapter 7.3 - "Service contact"). → 3.7.5 Temperature monitoring system If the temperature inside the rotor chamber rises above 50°C, the drive...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 4 Storage and transport → Storage and transport Dimensions and weight 1-16K, 1-16K IVD Height: 291 mm Height with open lid: 547 mm Width: 310 mm Depth: 660 mm Weight: 34 kg →...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 4 Storage and transport Packaging The centrifuge is packaged in a cardboard box. • Open the box. • Take out the box containing the accessories. • Lift the centrifuge with both foam cushions out of the cardboard box.
CAUTION Sigma centrifuges are units of protection class I. The centrifuges of this model series have a three-wire power cord with an IEC C13 connector. The removable power cord must not be longer than 3 m!
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 5 Set-up and connection 5.2.2 Customer-provided fuses Typically, the centrifuge must be protected with 16 Amp L or B fuses that are to be provided by the customer. To ensure safe disconnection in the event of a fault, an AC/DC-sensitive RCD (residual current device) must be integrated in the wiring system of the building.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge → Using the centrifuge → Initial start-up • Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 - "Set-up and connection").
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge 6.2.2 Installation of rotors and accessories 6.2.2.1 Installation of a rotor • Open the centrifuge lid by pressing the lid key. • Loosen the rotor tie-down screw by turning it anti-clockwise, but do not remove it.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge 6.2.2.2 Installation of a microhaematocrit rotor Fig. 5: Microhaematokrit rotor (part no. 11024) with reader (part no. 17029) • Open the centrifuge lid by pressing the lid key.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge 6.2.2.3 Installation of accessories • Only use tubes that are suitable for the rotor. • Always load the axial symmetrical inserts of the rotors with the same accessories and fill to avoid imbalance.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge Control System "Spincontrol Basic" 6.3.1 User interface Display Set key Program key Arrow keys Lid key Start/Stop key Precool key Fig. 7: User interface The centrifuge is started directly via the user interface. When the centrifuge is switched on, all segments will be illuminated for a short time.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge 6.3.3 Starting a centrifugation run The centrifuge is ready for operation when the mains power switch is on and the lid is closed. • Press the start/stop key in order to start a centrifugation run.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge 6.3.7 Runtime The preselected total runtime is displayed in the lower line of the display. During centrifugation, the remaining runtime is displayed. The runtime of the centrifuge can be set at one-second-intervals up to 99 minutes and 59 seconds.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge 6.3.7.3 Continuous run During the continuous run, the runtime of the centrifuge is unlimited and must be stopped manually. The centrifuge accelerates during the continuous run until the set speed is reached.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge Once the set value is reached and remains stable for one minute, the program ends with the indication "Precool OK". • Press the precool key to terminate the precooling program prematurely.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge 6.3.10 Rotor selection In the delivery status of the centrifuge, the rotor 12134 is preselected. If another rotor is installed the configuration must be changed, so that the allowed maximum speed of the rotor can be reached.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 6 Using the centrifuge 6.3.11 Program The program is used to save or load certain recurrent settings of the centrifuge. 10 different programs can be saved and called up. The precooling program "Precool" does not occupy any storage location and cannot be deleted.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 7 Malfunctions and error correction → Malfunctions and error correction General malfunctions Malfunctions are indicated by error messages with a number in the speed/RCF display. In the event of a fatal error (e.g. a defective lid lock), a certain safety time will be counted down on the display.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 7 Malfunctions and error correction 7.1.1 Emergency lid release In the event of a power failure, it is possible to manually open the centrifuge lid. • Switch off the mains power switch and disconnect the power cord from the socket.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 7 Malfunctions and error correction → Table of error codes Error no. Kind of error Measures Note • System error Allow to slow down All these errors stop the centrifuge or cause it to •...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 7 Malfunctions and error correction → Service contact In the event of queries, malfunctions, or spare part enquiries: From Germany: Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 8 Maintenance and service → Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 8 Maintenance and service → 8.1.1.1 Condenser (only refrigerated centrifuges with an air-cooled refrigeration system) In order to cool the refrigerant that is compressed by the refrigeration unit, centrifuges with an air-cooled refrigeration system use a lamellar condenser.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 8 Maintenance and service → 8.1.2.1 Plastic accessories The chemical resistance of plastic decreases with rising temperatures (see chapter 11.4 - "Resistance data"). • If solvents, acids, or alkaline solutions have been used, clean the plastic accessories thoroughly.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 8 Maintenance and service → 8.1.5 Glass breakage In the case of glass breakage, immediately remove all glass particles (e.g. with a vacuum cleaner). Replace the rubber cushions since even thorough cleaning will not remove all glass particles.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 8 Maintenance and service → 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use. • Replace the accessories immediately when the parts show changes in colour or structure or in the occurrence of leaks etc.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 8 Maintenance and service → Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury. • Only qualified specialist personnel is authorised to perform this service work.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 8 Maintenance and service → Return of defective centrifuges or parts Although we exercise great care during the production of our products, it may be necessary to return a unit or accessory to the manufacturer.
Page 49
NOTE The forms can be downloaded online from www.sigma-zentrifugen.de → [Service] → [Overhaul and repair]. Version 10/2013, Rev. 2.21 of 08/05/2020 • sb 49 / 70 Translation of the original operating manual, part no. 0700302...
Disposal → Disposal of the centrifuge In accordance with the directive 2012/19/EU, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household waste. •...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 10 Technical data → 10.1 Ambient conditions • The figures are valid for an ambient temperature of +23°C and a nominal voltage 10 %. The minimum temperature is ≤ +4°C and depends on the rotor type, speed, and ambient temperature.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 11 Appendix → 11 Appendix → 11.1 Range of accessories The complete list of accessories can be downloaded from www.sigma-zentrifugen.de. 11.1.1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 11 Appendix → 11.3 Table of the service life of rotors and accessories • If no other data concerning the service life are engraved on the rotor or accessory, rotors and buckets must be checked by the manufacturer after 10 years.
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 11 Appendix → 11.4 Resistance data The data refer to resistance at 20°C. NOTE - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde...
Page 58
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate...
Page 59
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate...
Page 60
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 11 Appendix Declaration of conformity – China RoHS 2 11.6 Version 10/2013, Rev. 2.21 of 08/05/2020 • sb 65 / 70 Translation of the original operating manual, part no. 0700302...
Page 66
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD 11 Appendix Version 10/2013, Rev. 2.21 of 08/05/2020 • sb 66 / 70 Translation of the original operating manual, part no. 0700302...
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD Index 12 Index Condenser, cleaning and care ....43 Contamination ........19, 43 Acceleration time Continuous heat resistance ......45 - see also the softstart and softstop function Continuous run .......... 34 ............
Page 68
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD Index Emergency lid release ....... 39 Error correction .......... 38 Layout of the centrifuge......11 Error messages ......... 38 Leaks ............46 Explanation of the symbols and notes ..16 Lid cannot be opened ........ 38 Explosive substances ........
Page 70
Refrigerated Centrifuge Sigma 1-16K Refrigerated Centrifuge Sigma 1-16K IVD Index Table of rotors and accessories with a Type..........12, 51, 52 different service life ......21, 56 Type of connection ........25 Technical data ........51, 52 Type of the centrifuge ......41, 47 Technical documentation ......
Need help?
Do you have a question about the 1-16K IVD and is the answer not in the manual?
Questions and answers